Influence of acetate-to-butyrate ratio on carbon chain elongation in anaerobic fermentation.

This study investigated the effect of electron acceptor (EA) distribution (acetate to butyrate ratio) on the carbon chain elongation (CCE) process. The results showed that the higher content of butyrate in the initial material led to the higher production of caproate. The maximum production of caproate was 3.

Compound mutation by ultraviolet and diethyl sulfate of protease producing thermophilic bacteria to hydrolyze excess sludge.

Excess sludge (ES), a resource-rich organic waste, can be solubilized by thermophilic enzymes to extract proteins for sludge reduction and resources recovery. To solve the problems of low hydrolysis effect of ES and low enzyme producing ability of wild thermophilic bacteria, ultraviolet and diethyl sulfate (UV-DES) were adopted to mutate thermophilic bacteria in this study. Mutation sites were detected and annotated by whole genome sequencing analysis.

Effect of homemade compound microbial inoculum on the reduction of terramycin and antibiotic resistance genes in terramycin mycelial dreg aerobic composting and its mechanism.

In order to tackle the issue of terramycin mycelial dreg (TMD) diagnosis and removal of terramycin and antibiotic resistance genes (ARGs), this study adopted aerobic composting (AC) technology and added homemade compound microbial inoculum (HCMI) to promote the AC of TMD and enhance the removal of terramycin and ARGs. The findings demonstrated that terramycin residue could be basically harmless after AC. Moreover, HCMI not only reduced QacB and tetH but also increased the degradation rates of VanRA, VanT, and dfrA24 by 40.

Effect of biochar on antibiotic resistance genes in the anaerobic digestion system of antibiotic mycelial dreg.

To address the problem of antibiotic mycelial dreg (AMD) treatment and removal of antibiotic resistance genes (ARGs), this study adopted anaerobic digestion (AD) technology, and added biochar (BC) and biochar loaded with nanosized zero-valent iron (nZVI-BC) to promote the AD of AMD and enhance the removal of ARGs. Results showed that nZVI-BC was better than BC in promoting AD due to the hydrogen evolution corrosion and the synergistic effect of nZVI and BC. In addition, BC and nZVI-BC can enhance the oxidative stress response and reduce ammonia stress phenomenon, which significantly reduces the abundance of aadA, ant(2″)-Ⅰ, qacEdelta1 and sul1.

Preparation of phosphoric acid modified antibiotic mycelial residues biochar: Loading of nano zero-valent iron and promotion on biogas production.

Antibiotic mycelial residues (AMRs), as recyclable hazardous waste, can realize efficient utilization by reasonable treatment. To solve the problems of undeveloped pore structure and low specific surface area existed in AMR biochar, this study first modified biochar by phosphoric acid (HPO) to prepare PBC (HPO-modified biochar). Then, PBC was used as carrier to load nano zero-valent iron (nZVI) for preparation of nZVI/PBC.

Novel insights from lignocellulosic waste to biogas through regulated dry-wet combined anaerobic digestion: Focusing on mining key microbes.

Dry-wet combined anaerobic digestion is a novel approach for treating lignocellulosic waste by increasing the organic load of reactor while accelerating the conversion of organic acids. Here, we investigated the effect of regulated substrate ratios and initial pH in the dry acidogenesis stage on the bioconversion efficiency of dry-wet combined anaerobic digestion. Our data revealed microbial interactions and further identified key microbes based on microbial co-occurrence network analysis.

Anaerobic digestion of lipid-rich swine slaughterhouse waste: Methane production performance, long-chain fatty acids profile and predominant microorganisms.

This study investigated methane production, long-chain fatty acids (LCFAs) profile, and predominant microorganisms in anaerobic digestion (AD) of lipid-rich swine slaughterhouse waste (SSW). The maximum methane yield was 999.2 mL/g VS.

Enhancement of methane production from Cotton Stalk using different pretreatment techniques.

China produces large amount of cotton stalk (CS) residues as agricultural biomass, which are incinerated on-site, causing air pollution. The high organic content of CS could be utilized for biogas production, but the direct digestion without pretreatment always leads to a low methane yield and biodegradability, due to the complicated structure of lignocellulose. In order to search best fitting pretreatment methods in effective anaerobic digestion (AD) of CS, effects of various pretreatments including KOH, NaOH, Ca(OH), alkali hydrogen peroxide (AHP), HSO, HPO and steam explosion (SE) were studied.