Protein expression patterns and metal metabolites in a protogynous hermaphrodite fish, the ricefield eel (Monopterus albus).

Background: The ricefield eel Monopterus albus undergoes a natural sex change from female to male during its life cycle, and previous studies have shown the potential mechanisms of this transition at the transcriptional and protein levels. However, the changes in protein levels have not been fully explored, especially in the intersexual stage.

Results: In the present study, the protein expression patterns in the gonadal tissues from five different periods, the ovary (OV), early intersexual stage gonad (IE), middle intersexual stage gonad (IM), late intersexual stage gonad (IL), and testis (TE), were determined by untargeted proteomics sequencing.

Expression Patterns and Gonadotropin Regulation of the TGF-β II Receptor (Bmpr2) during Ovarian Development in the Ricefield Eel .

Bmpr2 plays a central role in the regulation of reproductive development in mammals, but its role during ovarian development in fish is still unclear. To ascertain the function of in ovarian development in the ricefield eel, we isolated and characterized the cDNA sequence; the localization of Bmpr2 protein was determined by immunohistochemical staining; and the expression patterns of in ovarian tissue incubated with FSH and hCG in vitro were analyzed. The full-length cDNA was 3311 bp, with 1061 amino acids encoded.

Circular RNA expression profiles and CircSnd1-miR-135b/c-foxl2 axis analysis in gonadal differentiation of protogynous hermaphroditic ricefield eel Monopterus albus.

Background: The expression and biological functions of circular RNAs (circRNAs) in reproductive organs have been extensively reported. However, it is still unclear whether circRNAs are involved in sex change. To this end, RNA sequencing (RNA-seq) was performed in gonads at 5 sexual stages (ovary, early intersexual stage gonad, middle intersexual stage gonad, late intersexual stage gonad, and testis) of ricefield eel, and the expression profiles and potential functions of circRNAs were studied.

Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus).

Background: An increasing number of long noncoding RNAs (lncRNAs) have been found to play important roles in sex differentiation and gonad development by regulating gene expression at the epigenetic, transcriptional and posttranscriptional levels. The ricefield eel, Monopterus albus, is a protogynous hermaphroditic fish that undergoes a sequential sex change from female to male. However, the roles of lncRNA in the sex change is unclear.

Expression and regulation of Smad2 by gonadotropins in the protogynous hermaphroditic ricefield eel (Monopterus albus).

Smad2, a receptor-activated Smad, plays a critical role in regulating gametogenesis. In this study, a smad2 homologue was identified and sequenced from ricefield eel ovary cDNA, and its mRNA and protein expression levels were analysed during oocyte development. The cDNA sequence of ricefield eel smad2 consisted of 1863 bp encoding a 467-amino acid protein that had high sequence homology with Smad proteins in other teleosts, especially in Poeciliopsis prolifica.

Genetic analysis of wild in tributaries and the main stream of the upper Yangtze River basin of China.

Biodiversity is threatened by several factors that are often associated with overfishing, water pollution and hydroelectric dams, among other environmental impacts. The present study aimed to evaluate the genetic aspects of wild groups of using the mitochondrial cytochrome c oxidase subunit I () and cytochrome b () genes and the region. We collected 89 representative individuals from three geographically distinct ranges of the Upper Yangtze River, including the Longxi River (LOR), Laixi River (LAR), and Hejiang range of the Yangtze River (HJ).

The complete mitochondrial DNA of Herzensten and comparative mitogenomic analyses in species.

In the study, the complete mitochondrial genome of Herzensten was sequenced and compared with other species. The mitochondrial genome, consisting of 16,749 base pairs (bp), encoded 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and a noncoding control region, similar as that found in other species. These results can provide useful information for further studies on taxonomic status, molecular systematics, and stock evaluation.

The complete mitochondrial DNA of and comparative mitogenomic analyses of the species.

The complete mitochondrial genome of was sequenced and compared with others species. The mitochondrial genome, consisting of 16,680 base pairs (bp), encoded 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and a non-coding control region, as those found in other species. These results can provide useful data for further studies on taxonomic status, molecular systematics and stock evaluation.