Whole-genome analysis of a ST45-SCC IVa (2B)-t116 methicillin-resistant strain isolated from the sputum of a 5-year-old child with pneumonia.

Introduction: Methicillin-resistant (MRSA) sequence type (ST) 45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. In Hainan and Guangzhou of China, the ST45-MRSA was mainly associated with t116.

Methods: The MRSA strain SA2107 was isolated from the sputum of a 5-year-old child with pneumonia.

The Dynamics of Symbiodiniaceae and Photosynthetic Bacteria Under High-Temperature Conditions.

Coral thermal tolerance is intimately linked to their symbiotic relationships with photosynthetic microorganisms. However, the potential compensatory role of symbiotic photosynthetic bacteria in supporting Symbiodiniaceae photosynthesis under extreme summer temperatures remains largely unexplored. Here, we examined the seasonal variations in Symbiodiniaceae and photosynthetic bacterial community structures in Pavona decussata corals from Weizhou Island, Beibu Gulf, China, with particular emphasis on the role of photosynthetic bacteria under elevated temperature conditions.

Polystyrene microplastics exhibit toxic effects on the widespread coral symbiotic Cladocopium goreaui.

Within the coral reef habitat, members of the Symbiodiniaceae family stand as pivotal symbionts for reef-building corals. However, the physiological response of Symbiodiniaceae on microplastics are still poorly understood. Research conducted in this investigation assessed the harmful impact of polystyrene microparticles (PS-MPs) on Cladocopium goreaui, a Symbiodiniaceae species with a broad distribution.

An Efficient Printing Defect Detection Based on YOLOv5-DCN-LSK.

During the production process of inkjet printing labels, printing defects can occur, affecting the readability of product information. The distinctive shapes and subtlety of printing defects present a significant challenge for achieving high accuracy and rapid detection in existing deep learning-based defect detection systems. To overcome this problem, we propose an improved model based on the structure of the YOLOv5 network to enhance the detection performance of printing defects.

Simulations on coal water slurry gasification by molecular dynamics method with ReaxFF.

Context: Coal water slurry (CWS) is a new type of liquid coal product with low pollution, which is mainly used in the chemical industry to produce syngas (CO + H). It is of great significance to study the microscopic mechanism of CWS gasification reaction for improving the efficiency of coal gasification. In this paper, the method of molecular dynamics based on reaction force fields (ReaxFF-MD) is used to study the gasification process of CWS/O system at different temperatures.

Bacterial taxonomic and functional profiles from Bohai Sea to northern Yellow Sea.

Microbial distribution patterns are the result of a combination of biotic and abiotic factors, which are the core issues in microbial ecology research. To better understand the biogeographic pattern of bacteria in water environments from the Bohai Sea to the northern Yellow Sea, the effects of environmental factors, and spatial distance on the structure of bacterial communities in marine water were investigated using high-throughput sequencing technology based on 16S rRNA genes. The results showed that Proteobacteria, Bacteroidetes, Actinobacteri, Desulfobacterota, and Bdellovibrionota were the dominant phyla in the study area.

Leptin-deficient ob/ob mice exhibit periodontitis phenotype and altered oral microbiome.

Background And Objective: Leptin-deficient obesity is associated with various systemic diseases including diabetes and low bone mass phenotype. However, the periodontal status of leptin-deficient obese individuals is still unclear. In this study, we aimed to analyze the periodontal status, alveolar bone phenotype, and oral microbiome status in leptin-deficient obese mice (ob/ob mice).

regulates osteogenesis and bone mass phenotype via targeting gene.

MicroRNA-155 (miR155) is overexpressed in various inflammatory diseases and cancer, in which bone resorption and osteolysis are frequently observed. However, the role of miR155 on osteogenesis and bone mass phenotype is still unknown. Here, we report a low bone mass phenotype in the long bone of -Tg mice compared with wild-type mice.

Can Radiomics Provide Additional Information in [F]FET-Negative Gliomas?

The purpose of this study was to evaluate the possibility of extracting relevant information from radiomic features even in apparently [F]FET-negative gliomas. A total of 46 patients with a newly diagnosed, histologically verified glioma that was visually classified as [F]FET-negative were included. Tumor volumes were defined using routine T2/FLAIR MRI data and applied to extract information from dynamic [F]FET PET data, i.

Combination of pre-treatment dynamic [F]FET PET radiomics and conventional clinical parameters for the survival stratification in patients with IDH-wildtype glioblastoma.

Purpose: The aim of this study was to build and evaluate a prediction model which incorporates clinical parameters and radiomic features extracted from static as well as dynamic [F]FET PET for the survival stratification in patients with newly diagnosed IDH-wildtype glioblastoma.

Methods: A total of 141 patients with newly diagnosed IDH-wildtype glioblastoma and dynamic [F]FET PET prior to surgical intervention were included. Patients with a survival time ≤ 12 months were classified as short-term survivors.

Analysis of the expression profiles of long noncoding RNAs and messenger RNAs in tongue squamous cell carcinoma.

Objective: Long noncoding RNAs (lncRNAs) are involved in the progression of tongue squamous cell carcinoma (TSCC). Therefore, it is necessary to comprehensively investigate the role of lncRNAs in TSCC.

Study Design: In this study, RNA sequencing was performed to examine the expression profiles of lncRNAs and messenger RNAs (mRNAs) of patients with TSCC.

High Luminous Efficacy Phosphor-Converted Mass-Produced White LEDs Achieved by AlN Prebuffer and Transitional-Refraction-Index Patterned Sapphire Substrate.

Constant advance in improving the luminous efficacy () of nitride-based light-emitting diodes (LEDs) plays a critical role for saving measurable amounts of energy. Further development is motivated to approach the efficiency limit for this material system while reducing the costs. In this work, strategies of using thin AlN prebuffer and transitional-refraction-index patterned sapphire substrate (TPSS) were proposed, which pushed up the efficiency of white LEDs (WLEDs).

Local administration of low doses of exogenous BMP2 and leptin promotes ectopic bone regeneration in leptin-deficient mice.

Background: Obesity and leptin deficiency are associated with compromised bone regeneration.

Objective: This study aims to investigate the role of locally administrated low-dose BMP2+leptin on bone regeneration in leptin-deficient obese (ob/ob) mice.

Methods: Wildtype (WT) and ob/ob mice were divided into 3 groups (4 mice/group): BMP2 (5 μg) group, BMP2+low-dose leptin (1 μg) group, and BMP2+high-dose leptin (2.

Visualization of Combustion Phases of Biomass Particles: Effects of Fuel Properties.

This work established a high-speed camera-assisted visualization system that investigated the effect of volatile matter and fixed carbon content in biomass particles on single-particle combustion phases and their luminous properties. Three types of biomass particles, namely, sawdust (a mixture of pine and willow), corncob, and rice husk, were examined on a Hencken flat-flame burner. The luminous region and intensity of single biomass particles were closely related to the flammability and calorific value of biomass fuel and derived by analyzing a sequence of images captured using a high-speed camera.

Evaluation of facial soft tissue thickness in asymmetric mandibular deformities after orthognathic surgery.

Objectives: The purpose of this study was to compare differences in facial soft tissue thickness in three-dimensional (3D) images before and after orthognathic surgery in patients with skeletal Class III malocclusion and to obtain a better understanding of the relationship between hard and soft tissue changes after surgery.

Materials And Method: The present retrospective study included 31 patients with skeletal Class III malocclusion with mandibular chin deviation greater than 4 mm who had undergone cone-beam computed tomography before and 6 months after surgery. Seven bilateral points were established.

Prediction of TERTp-mutation status in IDH-wildtype high-grade gliomas using pre-treatment dynamic [F]FET PET radiomics.

Purpose: To evaluate radiomic features extracted from standard static images (20-40 min p.i.), early summation images (5-15 min p.

Combined inferior alveolar nerve block anaesthesia and local infiltration anaesthesia in extraction of impacted mandibular third molars: a randomised controlled trial.

Aim To compare the anaesthetic effect of combination of inferior alveolar nerve block anaesthesia and local infiltration anaesthesia with nerve block anaesthesia techniques in the extraction of mandibular third molars.Materials and methods One hundred and fifty healthy adult volunteers were divided into three groups and were administered three different local anaesthetic techniques before tooth extractions: group A (inferior alveolar nerve, lingual nerve and buccal nerve block anaesthesia with lidocaine), group B (inferior alveolar nerve, lingual nerve and buccal nerve block anaesthesia with mepivacaine) and group C (inferior alveolar nerve block anaesthesia with lidocaine and infiltration anaesthesia with mepivacaine). The patients' reported pain on anaesthesia injection and tooth extraction, the surgeon's assessment of anaesthetic effect, the severity of intra-operative bleeding and post-operative complications were evaluated.

Utilization of radiomics to predict long-term outcome of magnetic resonance-guided focused ultrasound ablation therapy in adenomyosis.

Objective: To develop and evaluate a T MR-based radiomics prediction model incorporating radiomics features and clinical parameters to predict the response to magnetic resonance-guided focused ultrasound surgery (MRgFUS) in patients with adenomyosis.

Materials And Methods: Sixty-nine patients (mean age, 38.6 years; age range, 26-50 years) with adenomyosis treated by MRgFUS were reviewed and allocated to training (n = 48) and testing cohorts (n = 21).

Potential impact of active substances in non-thermal discharge plasma process on microbial community structures and enzymatic activities in uncontaminated soil.

Soil microbial community structures and enzymatic activity are important indicators for judging biochemical process intensity and soil quality. Non-thermal discharge plasma (NDP), an advanced oxidation technique, has received great attention in soil remediation. Potential impact of NDP treatment on microbial community structures and enzymatic activities in uncontaminated soil samples was evaluated in this study.

Intelligent Nanocomposites with Intrinsic Blood-Brain-Barrier Crossing Ability Designed for Highly Specific MR Imaging and Sonodynamic Therapy of Glioblastoma.

The blood-brain barrier (BBB) is the most important obstacle to improving the clinical outcomes of diagnosis and therapy of glioblastoma. Thus, the development of a novel nanoplatform that can efficiently traverse the BBB and achieve both precise diagnosis and therapy is of great importance. Herein, an intelligent nanoplatform based on holo-transferrin (holo-Tf) with in situ growth of MnO nanocrystals is constructed via a reformative mild biomineralization process.

Single enzyme loaded nanoparticles for combinational ultrasound-guided focused ultrasound ablation and hypoxia-relieved chemotherapy.

Constructing nanosystems that synergistically combine therapeutic and diagnostic features is of great interest to the nanomedicine community but also remains a tremendous challenge. In this work, we report novel catalytic nanoparticles composed of the enzyme catalase, encapsulated in a polymer shell and surface decorated with pH-sensitive poly(ethylene glycol) (PEGylated nCAT). These nanoparticles were used as a promoter for ultrasound (US)-guided focused ultrasound (FUS) ablation and hypoxia alleviation for application in Doxorubicin-based chemotherapy.

mRNA and P-element-induced wimpy testis-interacting RNA profile in chemical-induced oral squamous cell carcinoma mice model.

P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs), a novel class of noncoding RNAs, are involved in the carcinogenesis. However, the functional significance of piRNAs in oral squamous cell carcinoma (OSCC) remains unknown. In the present study, we used chemical carcinogen 4-nitroquinoline-1-oxide (4NQO) induced OSCC mouse model.

Synthesis of BiWO nanodots with oxygen vacancies as an all-in-one nanoagent for simultaneous CT/IR imaging and photothermal/photodynamic therapy of tumors.

All-in-one nanoagents with a single-component and all-required functions have attracted increasing attention for the imaging-guided therapy of tumors, but the design and preparation of such nanoagents remain a challenge. Herein, we report the introduction of oxygen vacancies to traditional semiconductors with heavy-metal elements for tuning photoabsorption in the near infrared (NIR) region, by using Bi2WO6 (band-gap: ∼2.7 eV) as a model.