[Clinical efficacy of Manlyman Spray on premature ejaculation].

Objective: To observe the clinical effect of Manlyman Spray in the treatment of premature ejaculation (PE).

Methods: From January 2021 to March 2022, a total of 123 patients with PE were enrolled in clinical observation. Manlyman Spray was sprayed on the surface of the glans penis, corona of the glans and frenulum of the penis qd for 4 weeks.

[Cytogenetic and molecular analysis of a case of Prader-Willi syndrome].

Objective: To investigate the significance of cytogenetic and molecular genetic diagnosis of a special type of secondary sexual dysplasia and the applicability of various methods for its detection.

Methods: Using karyotype analysis, array comparative genomic hybridization (aCGH), multiplex ligation-dependent probe amplification (MLPA) and methylation-specific PCR (MS-PCR), we diagnosed and differentially diagnosed a case of secondary sexual dysplasia.

Results: Abnormalities were not found in the karyotype analysis or the SRY and AZF gene detection, nor chromosomal duplication and deletion in the initial SurePrint G3 Human CGH Array Kit8×60K.

[Application of array comparative genomic hybridization in prenatal diagnosis of a case with 5q35 deletion syndrome].

Objective: To use combined G-banding and array-comparative genomic hybridization (aCGH) for the prenatal diagnosis of a fetus with 5q35 deletion syndrome.

Methods: Chromosomal karotypes of the fetus and parents were analyzed with G-banding analysis. aCGH was performed to detect minor chromosomal structural abnormalities.

[Association of SPO11 and GST gene polymorphisms with idiopathic male infertility in ethnic Han Chinese].

Objective: To explore the possible roles of polymorphisms of SPO11 and glutathionine S-transferase (GST) genes in idiopathic male infertility in a ethnic Han Chinese population from Henan.

Methods: Multiplex PCR and DNA sequencing were performed to determine the SPO11 c.517C>T(rs28368082) and GST genes (GSTM1, GSTT1, GSTP1) polymorphisms in 216 idiopathic male infertility cases and 198 normal samples.