Flow injection chemiluminescence sensor using molecularly imprinted polymers as recognition element for determination of maleic hydrazide.

A novel flow injection chemiluminescence (CL) sensor for the determination of maleic hydrazide (MH) using molecularly imprinted polymer (MIP) as recognition element is reported. The MH-MIP was synthesized by thermal initiated polymerization in methanol, using methacrylic acid (MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker in the maleic hydrazide template molecule. Molecular modeling was employed to simulate the possible recognition process of the MIP, and to achieve high selectivity.

Quantitative determination of quinocetone and its metabolites in chicken plasma by liquid chromatography/tandem mass spectrometry.

A new method has been established for the simultaneous determination of the new veterinary drug quinocetone (3-methyl-2-cinnamoyl-N-1,4-dioxyquioxaline; QTN) and its 2 metabolites de-monoxy-quinocetone (3-methyl-2-cinnamoyl-N-1-monoxyquinoxaline; DMO-QTN) and de-dioxy-quinocetone (3-methyl-2-cinnamoyl-N-quinoxaline; DDI-QTN) in chicken plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The detection was performed using electrospray ionization in multiple reaction monitoring mode. The analysis of the linearity, accuracy, and precision of the method is described.

Simultaneous detection for three kinds of veterinary drugs: chloramphenicol, clenbuterol and 17-beta-estradiol by high-throughput suspension array technology.

Suspension array technology for simultaneous detection of three kinds of veterinary drugs, chloramphenicol (CAP), clenbuterol and 17-beta-estradiol has been developed. Conjugates of chloramphenicol and clenbuterol coupled with bovine serum albumin were synthesized and purified. Probes of suspension array were constituted by coupling the three conjugates on the fluorescent microspheres/beads and the microstructures of the beads' surface were observed by scanning electron microscopy which was a direct confirmation for the successful conjugates' coupling.

Preparation and characterization of bisphenol A-cationized bovine serum albumin.

To detect BPA residue in the environment quickly by immune method, preparation of high quality antibody against BPA was the most critical step of project. Based on Mannich-type reaction and EDC method, we produced BPA-cBSA and BHPVA-BSA against BPA and the conjugates were characterized by UV-Vis and FT-IR spectroscopy. Compared with BSA, cBSA as carrier protein could improve the efficiency of coupling and significantly enhance the immune response against BPA.

Immunochip for the detection of five kinds of chemicals: Atrazine, nonylphenol, 17-beta estradiol, paraverine and chloramphenicol.

This paper reports an immunochip for the detection of five kinds of chemicals: atrazine, nonylphenol, 17-beta estradiol, paraverine and chloramphenicol, which are harmful to human health and environment due to unconscionable use or abuse. Five antigen conjugates were synthesized, purified and then labeled with Cy3 dye. Meanwhile, the immunochip was prepared by immobilization the five kinds of specific antibodies on aldehydized slides as probes.

Influence of bisphenol a on developing rat estrogen receptors and some cytokines in rats: a two-generational study.

Bisphenol A (BPA) is an industrial chemical with estrogenic activity. In our study, 40 rats were given BPA at 4, 40, and 400 mg/kg per day. Controls were treated with corn oil of same volume until the offspring were 30 d olds.

Recognition of Staphylococcus enterotoxin via molecularly imprinted beads.

The synthesis of molecularly imprinted beads for the recognition of the protein Staphylococcus enterotoxin B (SEB) is described. Two kinds of organic silane (3-aminopropyltrimethoxysilane (APTMS) and octyltrimethoxysilane (OTMS)) were polymerized on the surface of polystyrene microspheres after the SEB template was covalently immobilized by forming imine bonds. The resulting imprinted beads were selective for SEB.

Detection of SEB gene by bilayer lipid membranes nucleic acid biosensor supported by modified patch-clamp pipette electrode.

This work reports a kind of novel bilayer lipid membranes (BLMs) nucleic acid biosensor supported by modified patch-clamp pipette electrode was developed to detect staphylococcus enterotoxins B (SEB) gene. BLMs were formed within 15 min and able to be operated at least 24 h. Hydrophobic dodecane tail (C12) modified 18 bp single-stranded DNA (ssDNA) probe was immobilized on BLMs.

Quartz crystal microbalance for the determination of daminozide using molecularly imprinted polymers as recognition element.

As the daminozide (DM) and its metabolite have been identified to be potentially carcinogenic, rapid detection method for them is necessary for food safety. A type of piezoelectric crystal sensor has been prepared by using a molecularly imprinted polymer (MIP) as recognition element. The molecularly imprinted polymer was prepared by hot-induced precipitation polymerization, and then the polymer particles were fixed on the surface of the electrode.

[Preparation and activity analysis of RGD-mSAK (K130T, K135R)].

In order to construct RGD-mSAK mutant with reduced immunogenicity, and identify its biological activity after purification, mSAK gene fragment was amplified by over-lapping extension PCR. Then the gene was inserted into the prokaryotic expression vector pBV220 with P(R)P(L) promoters after confirmed by DNA sequencing; the expression plasmid pBV220-RGD-mSAK was constructed, and then was transformed into E. coli.

[Progress of molecular imprinting technique on the determination of pesticides residues].

People have paid a more attention to the pesticides residues, so the rapid detection method is required. In this paper the application of molecular imprinting technique on the detection of pesticides residues was reviewed, including recognition principles, preparation, current applications, problems and its future.

Differentiation of rat neural stem cells and its relationship with environment.

Objective: To explore the differentiation fates of rat neural stem cells (NSCs) in different environmental conditions.

Methods: NSCs derived from 16-day-old rat embryo were proliferated in vitro and implanted into the brain of rats with intra-cerebral hemorrhage. At the same time some NSCs were co-cultured in vitro with Schwann cells derived from newborn rats.

[Advance of pesticide residue analysis techniques based on biological principle].

The application of pesticide residue analysis techniques based on biological principle, such as immunoassay, biosensor and direct test the influence of pesticide upon the activity of the enzyme and the growth of the propagation, was reviewed in this paper. Immunoassay is a kind of precise and sensitive method, but it's not easy to produce the antibody of pesticide. The strongpoint of biosensor is the process of the detection is quickly and real time, but the sensitivity and stability of biosensor still need improve.

[Flow injection analysis technique and its application in food analysis].

The review is based on the achievement and progress reported of food analysis with the flow injection analysis technique during 1990-2000 which contains the fundamental principle, unsolved problem, research and application on FIA technique.