[The effect of macrophage colony stimulating factor on laryngeal cancer Hep-2 cell line].

Objective: To investigate the possible role of M-CSF and its receptor in the laryngeal carcinoma Hep-2 cell line.

Method: Cell proliferation was assayed by MTT analysis and [3H] thymidine incorporation, after Hep-2 cell line culturing with M-CSF and M-CSFR. M-CSF concentration of surpernatant measured by a ELISA technique.

[Long-term results of tracheoesophageal puncture for voice restoration after total laryngectomy].

Objective: To evaluate the long-term results of tracheoesophageal puncture for Blom-Singer voice restoration after total laryngectomy and summarize our clinical experience of fifteen years.

Method: From 1986 to 2001, one hundred and thirty four cases with Blom-Singer technique after total laryngectomy for voice restoration were analyzed retrospectively. MDVP of Kay Elemetrics was used to analyze the acoustic parameters of eighteen cases with Blom-Singer indwelling voice prosthesis for 2-14 years.

Electrochemical activation of glucose oxidase with a 140-fold enhancement in intramolecular electron transfer rate constant.

In this work, we describe the electrochemical activation of glucose oxidase (GOx) via covalent attachment of a novel redox mediator, Os(bpy)2(API)Cl (bpy = 2,2'-bipyridine, API = 3-aminopropylimidazole), to the peptide backbone of GOx targeting at aspartate and glutamate residues. Cyclic voltammetry showed a pair of well-defined voltammetric peaks centered at 0.11 V for the activated enzyme.

An interference-free implantable glucose microbiosensor based on use of a polymeric analyte regulating membrane.

An implantable glucose microbiosensor that utilizes an electrodeposited redox polymer/glucose oxidase (GOX) sensing membrane and a highly hydrophilic poly (4-vinyl pyridine-co-acrylic acid) (PVP-PAC) overcoating as an analyte-regulating membrane has been developed. A gold microelectrode was electrodeposited with the redox polymer/GOX composite sensing membrane. Two polymers, a hydrophobic poly (4-vinyl pyridine) (PVP) and a hydrophilic PVP-PAC were investigated as possible analyte-regulating membranes.

An ultrasensitive protein array based on electrochemical enzyme immunoassay.

An ultrasensitive electrochemical enzyme immunoassay (EEIA) for the detection of proteins on an 8x8 array is described. The assay is based on wired enzyme technology. Briefly, capture antibody was covalently immobilized on a self-assembled 11-mercaptoundecanoic acid (MUA) monolayer coated gold electrode.

Effects of emodin on treating murine nonalcoholic fatty liver induced by high caloric laboratory chaw.

Aim: To investigate the effects of emodin on the treatment of non-alcoholic fatty liver in rats induced by high caloric laboratory chaw.

Methods: Non-alcoholic fatty liver model was successfully established by feeding with high caloric laboratory chaw for 12 wk. Then the model rats were randomly divided into 3 groups, namely model control group, emodin group and dietary treatment group.

[Study on therapeutic effects of metformin on rat fatty livers induced by high fat feeding].

Objectives: To explore the therapeutic effects of metformin on rat fatty livers induced by high fat feeding.

Methods: A fatty liver model was established by feeding rats with a high caloric laboratory chow for 12 weeks, then the rats were randomly divided into three groups, i.e.

An ultrasensitive nucleic acid biosensor based on the catalytic oxidation of guanine by a novel redox threading intercalator.

An ultrasensitive nucleic acid biosensor for the direct detection of attomoles nucleic acid in 1.0-5.0 [corrected] microl droplets is described which can be used for detection of cancer marker genes in mRNA extracted from human breast tissues without a RT-PCR step.

Direct detection of DNA with an electrocatalytic threading intercalator.

Herein we report the synthesis, intercalating properties, and analytical applications of an imidazole-substituted naphthalene diimide, N,N'-bis(3-propylimidazole)-1,4,5,8-naphthalene diimide (PIND), functionalized with electrocatalytic redox moieties. PIND was prepared in a single-step reaction from the corresponding dianhydride. Attachment of the redox moieties to PIND relied upon ligand exchange with one of the liable chloride ligands of an Os(bpy)2Cl2 (bpy = 2,2'-bipyridine) complex.

[Diagnosis and therapy of glomus tympanicum and glomus jugulare tumors].

Objective: To report on a series of patients with glomus tympanicum or glomus jugulare tumors, and to focus on its diagnosis, treatment, and outcomes.

Methods: Ten patients with glomus tympanicum or glomus jugulare tumors at Peking Union College Hospital during a 17-year period were reviewed retrospectively.

Results: There were 7 patients with glomus tympanicum, 3 with glomus jugulare tumors, 1 patient with familial paraganglioma and 1 with functioning glomus jugulare tumors.

A nucleic acid biosensor for gene expression analysis in nanograms of mRNA.

An ultrasensitive nucleic acid biosensor for direct detection of genes in mRNA extracted from animal tissues is described. It is based on amperometric detection of a target gene by forming an mRNA/redox polymer bilayer on a gold electrode. The mRNA was directly labeled with cisplatin-biotin conjugates through coordinative bonds with purine bases in the mRNA molecules.

Telomerase activity of peripheral blood mononuclear cells from patients with laryngeal carcinoma.

This study investigated the activity of telomerase in peripheral blood mononuclear cells (PBMCs) in patients with laryngeal carcinoma. We examined proliferative response to phytohemagglutinin and expression of telomerase activity of PBMCs from 30 patients with laryngeal carcinoma and 17 healthy volunteers. Both proliferative and telomerase activity of [circled times]PBMCs in the patient group was lower than that in the healthy volunteer group.

[The use of muscle autograft denatured by microwave for repair of gaps in removal of facial neuromas].

Objective: To study the clinical value of muscle autograft denatured by microwave for repair of gaps in removal of facial neuromas.

Methods: Two cases of patients with facial nerve Schwann cell neuromas were reported. The operations for removal of facial neuromas were completed, and the gaps of the nerves were repaired with muscle autograft denatured by microwave of 250 W for 120 sec.

Amperometric detection of nucleic acid at femtomolar levels with a nucleic acid/electrochemical activator bilayer on gold electrode.

Cationic redox polymers containing osmium-bipyridine complexes strongly interact with anionic enzymes, such as glucose oxidase and peroxidases, and electrochemically "activate" the enzymes. On the basis of these observations, attempts were made to develop an ultrasensitive nucleic acid biosensor. A mixed monolayer of single-stranded oligonucleotide capture probe and 16-mercaptohexadecanoic acid was formed on a gold electrode through self-assembly.

Highly sensitive amperometric detection of genomic DNA in animal tissues.

A simple and highly sensitive method for the detection of genomic DNA in tissue samples is described. It is based on amperometric detection of target DNA by forming an analyte/polymeric activator bilayer on a gold electrode. The biotinylated target DNA is hybridized to oligonucleotide capture probes immobilized on the gold electrode, forming the first layer.

An amperometric biosensor for glucose based on electrodeposited redox polymer/glucose oxidase film on a gold electrode.

In this paper, we described a glucose biosensor based on the co-electrodeposition of a poly(vinylimidazole) complex of [Os(bpy)2Cl](+/2+) (PVI-Os) and glucose oxidase (GOX) on a gold electrode surface. The one-step co-electrodeposition method provided a better control on the sensor construction, especially when it was applied to microsensor construction. The modified electrode exhibited the classical features of a kinetically fast redox couple bound to an electrode surface and the redox potential of the redox polymer/enzyme film was 0.

[The use of muscle autograft denatured by microwave for repair of gap in removal of facial neuroma].

Objective: To study the clinical value of muscle autograft denatured by microwave for repair of gap in removal of facial neuroma.

Methods: A case of patient with a larger facial nerve Schwann cell neuroma was reported. Based on the good results of our experimental research on rats recently, we completed the operation by transmastoid approach for removal of facial neuroma, and the gap of the nerve was repaired with muscle autograft denatured by microwave.

[Pharyagoesophageal sphinoter myotomy for voice rehabilitation after total laryngectomy].

Objective: In those patients received total laryngectomy, Blom-Singer prosthesis speech appears to be the more effective form of rehabilitation. To reduce the effect of pharyngoesophageal sphincter on the Blom-Singer prosthesis voice restoration following total laryngectomy, the pharyagoesphageal sphincter myotomy was designed and carried out.

Methods: After total laryngectomy, the cricopharyngeus muscle stricture or spasm appears to inhibit the air flowing and prevent the speech.

From linear to nonlinear control means: a practical progression.

With the rapid advance of digital control hardware, it is time to take the simple but effective proportional-integral-derivative (PID) control technology to the next level of performance and robustness. For this purpose, a nonlinear PID and active disturbance rejection framework are introduced in this paper. It complements the existing theory in that (1) it actively and systematically explores the use of nonlinear control mechanisms for better performance, even for linear plants; (2) it represents a control strategy that is rather independent of mathematical models of the plants, thus achieving inherent robustness and reducing design complexity.