Exploring gut microbiota and metabolite alterations in patients with thyroid-associated ophthalmopathy using high-throughput sequencing and untargeted metabolomics.

Introduction: Thyroid-associated ophthalmopathy (TAO) is an autoimmune-driven orbital inflammatory disease. Despite research efforts, its exact pathogenesis remains unclear. This study aimed to characterize the intestinal flora and metabolic changes in patients with TAO to identify the flora and metabolites associated with disease development.

Difference between arterial and venous peak optical density after thrombectomy is associated with functional outcomes.

Background: The density of contrast medium in digital subtraction angiography (DSA) have been used to evaluate the cerebral circulation function. Our aim was to study the effect of difference in arteriovenous peak optical density (POD) after thrombectomy on functional outcomes.

Methods: Consecutive patients with acute ischemic stroke due to large vessel occlusion who underwent thrombectomy were reviewed.

Prognosis and Prediction of Asymptomatic Intracranial Hemorrhage After Endovascular Thrombectomy: A Multi-Center Study.

Purpose: The impact of asymptomatic intracranial hemorrhage (aICH) on functional outcomes after endovascular thrombectomy (EVT) remains unclear, and tools for forecasting this complication are lacking. We aim to evaluate the clinical relevance of aICH and establish a prediction model.

Methods: Data of patients who received EVT for acute anterior-circulation large vessel occlusion in 3 comprehensive hospitals were retrospectively analyzed.

Case report: Retrograde endovascular recanalization of vertebral artery occlusion with non-tapered stump via the deep cervical collateral.

Introduction: Vertebral artery (VA) occlusive disease is the major cause of posterior circulation ischemic stroke. Endovascular recanalization has been reported as a feasible treatment for patients with symptomatic VA occlusion refractory to optimal medical therapy. However, VA occlusion with non-tapered stump exhibits a low technique success rate when treated by antegrade endovascular therapy because of increased difficulty in passing the guidewire into the occluded segment.

Whole-genome sequencing of species isolated from the healthy human eye and description of sp. nov.

Currently, the genus comprises 76 recognized species. Members of are mostly isolated from environmental, animal, and plant sources. This report describes and proposes a novel species of isolated from clinical specimens of the human ocular surface.

Characteristics and associations of microbial community in the eye, anterior nares, and oropharynx of healthy adults: eye-nose microbiota transmission.

Purpose: We compared the microbial communities of the eye, anterior nares (ANs) and oropharynx (OP) of healthy adults to provide a basic understanding of the microbial associations among the three sites.

Methods: The name of the registry of our prospective study was "Study on the diversity of microbial flora in the eye, nose and oropharynx of healthy people". The trial number is ChiCTR2300067724 (https://www.

Effects of different planting distances and fertilizer use on the remediation of farmland contaminated with Cd by intercropping Cucurbita moschata and Amaranthus hypochondriacus L.

Selecting suitable agronomic measures can strengthen the application of intercropping in the remediation of cadmium (Cd)-contaminated soil. In this study, the effects of different planting densities and fertilizer applications on the crop growth and Cd absorption of a pumpkin (Cucurbita moschata)-Amaranthus hypochondriacus L. intercropping system was determined.

Proximal hyperdense middle cerebral artery sign is associated with increased risk of asymptomatic hemorrhagic transformation after endovascular thrombectomy: a multicenter retrospective study.

Objective: To investigate whether hyperdense middle cerebral artery sign (HMCAS) on pretreatment no-contrast CT (NCCT) is associated with hemorrhagic transformation (HT) after endovascular thrombectomy (EVT).

Methods: Patients with acute middle cerebral artery (MCA) occlusion who received EVT in three comprehensive hospitals were retrospectively evaluated. They were divided into four groups based on the presence or absence of HMCAS and corresponding CTA findings, among whom differences were compared.

The species-level microbiota of healthy eyes revealed by the integration of metataxonomics with culturomics and genome analysis.

Objectives: To characterize the healthy ocular surface microbiota at the species level, including cultured and uncultured taxa.

Methods: We integrated the metataxonomic method with culturomics and genome sequencing analysis of selected isolated strains to better illustrate the taxonomic structure of the ocular surface microbiota. The metataxonomics used the full-length 16S rRNA gene sequences and the operational phylogenetic unit strategy, which can precisely identify the cultured and uncultured or potentially new taxa to species level based on the phylogenetic tree constructed.

Application of High-Throughput Sequencing Technology in Identifying the Pathogens in Endophthalmitis.

Infectious endophthalmitis is an important cause of vision loss worldwide. It is an inflammatory reaction caused by bacteria, fungi, and other micro-organisms and often occurs as a complication of intraocular surgery, especially following cataract surgery or intravitreal injection. The focus of the prevention and treatment of infectious endophthalmitis is the early detection of microbial flora, such as fungi or bacteria.

Interplanting of rice cultivars with high and low Cd accumulation can achieve the goal of "repairing while producing" in Cd-contaminated soil.

Interplanting has been highlighted as a promising, cost-effective, and environmentally friendly solution for the remediation of contaminated soil. In this study, field experiments were conducted to study growth and cadmium (Cd) uptake in monoculture and interplanting systems with rice varieties Changliangyou 772 (C-772) and Changxianggu (Cho-ko-koku). And a pot culture experiment was conducted to investigate the response of the rhizosphere microecology of these two rice varieties.

Comparison of the ocular surface microbiota between thyroid-associated ophthalmopathy patients and healthy subjects.

Purpose: Thyroid-associated ophthalmopathy (TAO) is a chronic autoimmune disease. In this study, high-throughput sequencing was used to investigate the diversity and composition of the ocular microbiota in patients with TAO.

Methods: Patients with TAO did not receive treatment for the disease and did not have exposed keratitis.

A Nomogram for Predicting Symptomatic Intracranial Hemorrhage after Endovascular Thrombectomy.

Background: Symptomatic intracranial hemorrhage (sICH) is a devastating complication of endovascular thrombectomy (EVT). We aim to develop and validate a nomogram for predicting sICH in patients with large vessel occlusion (LVO) in the anterior circulation.

Methods: We performed a single-center retrospective analysis on collected data from patients undergoing EVT for LVO in the anterior circulation between January 2018 and December 2021.

Posterior Circulation ASPECTS on CT Angiography Predicts Futile Recanalization of Endovascular Thrombectomy for Acute Basilar Artery Occlusion.

Background: Acute basilar artery occlusion (BAO) is the most potentially disastrous outcome and has a high risk of recurrence stroke in posterior circulation infarction (PCI). However, the rate of futile recanalization remains high despite successful recanalization. The objective of this study was to investigate 90 days functional outcomes among patients with BAO who underwent endovascular thrombectomy (EVT) and to identify the risk factors associated with futile recanalization.

Clot Meniscus Sign Is Associated With Thrombus Permeability and Choice of Mechanical Thrombectomy Technique in Acute Middle Cerebral Artery Occlusion.

Background And Purpose: The method of mechanical thrombectomy (MT) is related to vascular anatomy and stroke etiology. Meniscus sign and thrombus permeability as imaging markers may be instructive for the selection of MT. This study aims to clarify the relationship among meniscus sign, thrombus permeability, and choice of MT in patients with acute middle cerebral artery occlusion.

[Impact of Meteorological Conditions on PM in Jiangsu Province from 2001 to 2019].

The weather research and forecasting model coupled with chemistry (WRF-Chem) was used to investigate the impact of meteorological conditions on PM in Jiangsu Province from 2001 to 2019. Under the condition of constant emission sources, the strongest positive and negative anomalies of annual PM concentration caused by meteorological conditions occurred in 2008 and 2001, respectively. Furthermore, their anomalies respectively accounted for 10.

A Loop-mediated Isothermal Amplification With a Nanoparticle-Based Lateral Flow Biosensor Assay to Detect Pseudomonas aeruginosa in Endophthalmitis.

Purpose: Pseudomonas aeruginosa is the most common bacteria causing endophthalmitis after cataract surgery. Vitreous fluid culture and molecular studies are commonly used in clinical diagnoses, but have disadvantages, such as a long culture cycle and low detection sensitivity. Here, we report a loop-mediated isothermal amplification (LAMP) method combined with the nanoparticles-lateral flow biosensor (LFB) method for rapid and specific detection of P.

Low Cd-accumulating rice intercropping with Sesbania cannabina L. reduces grain Cd while promoting phytoremediation of Cd-contaminated soil.

Paddy field pollution with Cd has become a serious problem and poses threat to public health. Intercropping is new good agricultural practice for phytoremediation in Cd contaminated soil. Field and pot experiments were conducted to examine the effects of intercropping low Cd-accumulating rice with Sesbania cannabina on plant growth, uptake of Cd by the intercropping system, and rhizosphere microecology, and to evaluate the potential remediation of Cd contaminated soil and safety production of rice.

Efficacy and Safety of Intravenous Thrombolysis on Acute Branch Atheromatous Disease: A Retrospective Case-Control Study.

Branch atheromatous disease (BAD) is distinctive from large-artery atherosclerosis and small-vessel disease, which is single subcortical infarction caused by the occlusion of perforator's orifice. This study aimed to indicate whether intravenous thrombolysis (IVT) with alteplase could prevent early neurological deterioration (END) and improve functional outcome for patients with BAD within 4.5 h after symptom onset.

Pollution characteristics and ecological risk assessment of heavy metals in paddy fields of Fujian province, China.

To analyze the concentration, spatial distribution patterns, and ecological risks of heavy metals (Cd, Cr, Pb, As, Cu, Ni and Co), 272 topsoil samples (0-20 cm) were collected from paddy fields in Fujian province in July 2017. The results revealed that the mean concentration of all heavy metals exceeded the background values in Fujian province, with the mean concentration of Cd being 5.20 times higher than its background.

A combinatorial strategy for overcoming primary and acquired resistance of MEK inhibition in colorectal cancer.

Compared with traditional chemotherapeutic drugs, targeted therapeutic medicine has the advantages of high efficacy and less toxic side effects. However, in clinical practice for treatment of colorectal cancer, the primary and acquired resistance of these medicines limits their effectiveness in targeted therapy, therefore impedes the development of precision medicine and personalized therapy. Currently, there are limited number of drugs for targeted therapy of colorectal cancer, mainly monoclonal antibodies against EGFR or VEGFR inhibitors.

Yield advantage and cadmium decreasing of rice in intercropping with water spinach under moisture management.

Rice (Oryza sativa L.) intercropping with water spinach (Ipomoea aquatica Forsk) is an effective agricultural practice for safe crop production and for phytoremediation in cadmium-contaminated soil. A field and pot experiment were conducted to investigate the growth and cadmium absorption of rice intercropped with water spinach under different moisture management schemes (continuous flooding, interval flooding, and 75% field capacity).

MRPL35 Is Up-Regulated in Colorectal Cancer and Regulates Colorectal Cancer Cell Growth and Apoptosis.

Mitochondrial ribosome proteins (MRPs), which are encoded by the nuclear genomic DNA, are important for mitochondrial-encoded protein synthesis and mitochondrial function. Emerging evidence suggests that several MRPs also exhibit important extra-mitochondrial functions, such as involvement in apoptosis, protein biosynthesis, and signal transduction. In this study, we demonstrate a significant role of MRP L35 (MRPL35) in colorectal cancer (CRC).

RasGRF2 promotes migration and invasion of colorectal cancer cells by modulating expression of MMP9 through Src/Akt/NF-κB pathway.

Ras-specific guanine nucleotide-releasing factor 2 (RasGRF2) is a member of the guanine nucleotide exchange factors family which is expressed in a variety of tissues and cancer. However, the role of RasGRF2 in cancer is less reported, especially in colorectal cancer(CRC). Hence, the present study aimed to investigated the function of RasGRF2 and ways in which it affects tumor progression in CRC samples and cell lines.