miR-374-5p inhibits osteogenesis by targeting PTEN/PI3K/AKT signaling pathway.

Purpose: This study aims to evaluate the effects of miR-374-5p on osteogenesis in rat osteoblasts, validate its target on PTEN, and explore its role in the PTEN/PI3K/AKT signaling pathway during osteoblast differentiation.

Methods: We transfected 293T cells with miR-374-5p mimics and inhibitors, followed by Western blot and qRT-PCR analyses to assess protein and mRNA expression levels. A dual-luciferase assay was performed to confirm direct targeting.

Two different fabrication method of high-hydrophobic Bi-layer indicators based on sodium alginate-polyvinyl alcohol/chitosan-zein incorporated with alizarin@ZIF-8 for beef freshness visualization.

Intelligent films are essential for real-time monitoring of food spoilage. However, the application is often limited by challenges such as hydrophilicity and color instability. In this study, two high hydrophobic bilayer indicators based on sodium alginate-polyvinyl alcohol/chitosan-zein with alizarin grafted onto ZIF-8 carrier (AL@ZIF-8) were fabricated using the casting method and electrospinning, respectively.

Expert consensus on the prevention and treatment of enamel demineralization in orthodontic treatment.

Enamel demineralization, the formation of white spot lesions, is a common issue in clinical orthodontic treatment. The appearance of white spot lesions not only affects the texture and health of dental hard tissues but also impacts the health and aesthetics of teeth after orthodontic treatment. The prevention, diagnosis, and treatment of white spot lesions that occur throughout the orthodontic treatment process involve multiple dental specialties.

Advances in 3D and 4D Printing of Gel-Based Foods: Mechanisms, Applications, and Future Directions.

This review examines recent advancements in gel-based 3D and 4D food-printing technologies, with a focus on their applications in personalized nutrition and functional foods. It emphasizes the critical role of tunable rheological and mechanical properties in gels such as starch, protein, and Pickering emulsions, which are essential for successful printing. The review further explores 4D food printing, highlighting stimuli-responsive mechanisms, including color changes and deformation induced by external factors like temperature and pH.

A rare case of vision loss caused by leptomeningeal metastasis of lung adenocarcinoma: a case report and literature review.

Leptomeningeal metastasis (LM) is a fatal complication with increasing incidence in patients with non-small cell lung cancer. Herein, we present the case of a patient who presented with complete vision loss due to LM and achieved a survival benefit from treatment with trametinib. The treatment was prescribed based on the detection of a BRAF non-V600E mutation in the cerebrospinal fluid (CSF).

As(III) removal from drinking water using FMCTO@FeO in the adsorption-magnetic separation-sand filtration equipment: Trade-off between As removal efficiency and adsorbent utilization rate.

Due to the carcinogenicity and high environmental mobility of arsenic (As), its contamination in the groundwater environment is widespread, continuously threatening human health through the food chain. The adsorption technologies for As removal, which demonstrate simplicity and cost-effectiveness, have received much attention. Despite these merits, the difficult separation between adsorbent and As-contaminated water in traditional adsorption limited the development of large-scale applications.

Lemon-derived carbon quantum dots incorporated guar gum/sodium alginate films with enhanced the preservability for blanched asparagus active packaging.

Minimally processed fruits and vegetables (MPFVs) experience significant quality degradation during storage due to oxygen exposure, mechanical damage, and microbial contamination, which significantly reduces shelf life and leads to substantial economic waste. This research developed a cost-effective and environmentally active packaging by incorporating carbon quantum dots (CQDs) derived from lemons into guar gum (GG) and sodium alginate (SA) films. The CQDs were integrated into the biopolymer matrix via simple film-casting techniques.

Simultaneous adsorption and fluorescent sensing of ampicillin based on a trimetallic metal-organic framework.

Antibiotic residue is a growing concern for human health, and exploring a method for antibiotic detection is imperative Herein, a trimetallic metal-organic framework (NH2-MIL-53(Al)@Mo/Zn-MOF) with an adsorption effect was prepared by growing Mo/Zn-MOF on the surface of NH-MIL-53(Al) for simultaneous pre-concentration and detection of ampicillin (AMP). The NH-MIL-53(Al)@Mo/Zn-MOF showed a large specific surface area and stable crystal structure, which is conducive to improving the adsorption efficiency and detection sensitivity. The adsorption process of NH-MIL-53(Al)@Mo/Zn-MOF to AMP was simulated by a quasi-second-order kinetic model and Langmuir model.

Fate of polystyrene micro- and nanoplastics in zebrafish liver cells: Influence of protein corona on transport, oxidative stress, and glycolipid metabolism.

Micro- and nanoplastics (MNPs) form protein corona (PC) upon contact with biological fluids, but their impact on the intracellular transport, distribution, and toxicity of MNPs remains unclear. Fetal bovine serum (FBS) and bovine serum albumin (BSA) were used to simulate in vivo environment, this study explored their influence on the transport and toxicity of polystyrene (PS) MNPs in zebrafish liver (ZFL) cells. Results showed PS MNPs were wrapped by proteins into stable complexes.

Application of Smart Packaging in Fruit and Vegetable Preservation: A Review.

The application of smart packaging technology in fruit and vegetable preservation has shown significant potential with the ongoing advancement of science and technology. Smart packaging leverages advanced sensors, smart materials, and Internet of Things (IoT) technologies to monitor and regulate the storage environment of fruits and vegetables in real time. This approach effectively extends shelf life, enhances food safety, and reduces food waste.

Metabolomics, network pharmacology, and microbiome analyses uncover the mechanisms of the Chinese herbal formula for the improvement of meat quality in spent hens.

Background: Meat originating from the spent hen is an important source of poultry meat production; however, multiple factors cause the decline in the meat quality of spent hens. Chinese herbs have been widely used as medicine for a long time to prevent diseases and as nutrient supplements to improve the product quality. This experiment explored the effects of adding 1.

Atractylenolide I prevents acute liver failure in mouse by regulating M1 macrophage polarization.

Acute liver failure (ALF) is a life-threatening clinical syndrome with a substantial risk of mortality. A murine model of lipopolysaccharide (LPS)- and D-galactosamine (D-GalN)-induced ALF is widely used to investigate the underlying mechanisms and potential therapeutic drugs for human liver failure. Atractylenolide I (ATR-I) is an active component of the Atractylodes macrocephala rhizome and possesses various pharmacological activities, including anti-tumor, anti-inflammatory, and anti-oxidant properties.

Preparation of photodynamic-controlled release packaging for pork preservation and its visualization.

The current study aimed to synthesize a ZIF-8 metal-organic framework loaded with the Rose Bengal (ZIF-8@RB) as the photodynamic sterilization ingredient to address the uncontrolled release of active ingredients in packaging films. The photodynamic controlled release packaging film was then prepared using a PVDF polymer matrix via uniaxial electrospinning. The microstructure, particle size, excitation wavelength, and singlet oxygen yield of ZIF-8@RB were examined.

Toward Developing Alternative Opioid Antagonists for Treating Community Overdose: A Model-Based Evaluation of Important Pharmacological Attributes.

In response to increased illicit use of synthetic opioids, various μ-receptor antagonist formulations, with varied pharmacological characteristics, have been and are being developed. To understand how pharmacologic characteristics such as absorption rate and clearance rate affect reversal in treating community opioid overdose, we used our previously published translational opioid model. We adapted this model with in vitro receptor binding data and clinical pharmacokinetic data of three intranasal nalmefene formulations along with an intranasal naloxone formulation to study the reversal of fentanyl and carfentanil-induced respiratory depression in chronic opioid users.

Robot-Assisted Radical Prostatectomy Using the KangDuo Surgical Robot-1500: A Prospective, Multicenter, Single-Arm Clinical Study.

The KangDuo Surgical Robot-1500 (KD-SR-1500) is a newly developed surgical robot. We aim to evaluate the feasibility and efficiency of the KD-SR-1500 system for robot-assisted radical prostatectomy (RARP). This prospective, multicenter, single-arm clinical study was conducted among 18-75-year-old patients with suspected T1-2N0M0 prostate cancer scheduled for RARP.

Structure and metabolic function of spatiotemporal pit mud microbiome.

Background: Pit mud (PM) hosts diverse microbial communities, which serve as a medium to impart flavor and quality to Baijiu and exhibit long-term tolerance to ethanol and acids, resulting in a unique ecosystem. However, the ecology and metabolic functions of PM remain poorly understood, as many taxa in PM represent largely novel lineages. In this study, we used a combination of metagenomic analysis and chemical derivatization LC-MS analysis to provide a comprehensive overview of microbial community structure, metabolic function, phylogeny, horizontal gene transfer, and the relationship with carboxyl compounds in spatiotemporal PM samples.

Distinct Effect of Benzophenone-3 Additive Leaching from Polyethylene Microplastics on Daphnia magna Population Dynamics.

The adverse effect of chemical additives leaching from microplastics (MPs) on Daphnia magna populations is not fully understood. In this study, D. magna populations were exposed to polyethylene (PE) MP fragments (5.

Thoracoscopic anatomical sublobar resection for deep interlobar lung cancer with fused fissure.

Background: The technical challenges associated with the removal of small nodules in challenging locations rather than peripheral locations remain unaddressed. We sought to illustrate the parenchymal-sparing surgical approach employed for deep interlobar lung cancer with fused fissures (DILCFFs).

Methods: A retrospective review of 43 patients with cT1N0M0 DILCFFs from January 2013 through December 2022 was performed.

Effects of the Salvia miltiorrhiza, Ligustrum lucidum, and Taraxacum mongolicum ultra-fine powder formula on meat quality of aged layers by multi-omics.

This study was aimed to identify the targets of 1% ultra-fine Chinese medicine formula (UCMF, 0.5% Salvia miltiorrhiza Bge. + 0.

The Necessity of Lymphadenectomy for Non-Small Cell Lung Cancer Smaller Than 6 mm in Solid Size.

Background: This study aimed to evaluate the necessity of lymph node sampling for specific non-small cell lung cancer (NSCLC).

Methods: Patients with small-size (≤2 cm) NSCLC who underwent surgical resection between 2009 and 2022 were retrospectively screened. The characteristics of patients with nodal metastasis were demonstrated.

A Nomogram for Predicting Perirenal Hematoma After Percutaneous Nephrolithotomy.

Backgroud: Perirenal hematoma (PRH) is a notable complication following percutaneous nephrolithotomy (PCNL) with significant implications for patient outcomes. This study aimed to develop a nomogram predictive model for PRH after PCNL.

Methods: Retrospective data from patients who underwent PCNL were analyzed.

Glecirasib in KRAS-mutated nonsmall-cell lung cancer: a phase 2b trial.

Glecirasib (JAB-21822) is a new covalent oral KRAS-G12C inhibitor. This multicenter, single-arm phase 2b study assessed the efficacy and safety of glecirasib administered orally at 800 mg daily in patients with locally advanced or metastatic KRAS-mutated nonsmall-cell lung cancer. The primary endpoint was the objective response rate (ORR) assessed by an independent review committee (IRC).

Novel Insights from Comprehensive Bioinformatics Analysis Utilizing Large-Scale Human Transcriptomes and Experimental Validation: The Role of Autophagy in Periodontitis.

Objective: Autophagy plays a crucial role in the pathophysiology of periodontitis, yet its precise involvement in the disease process remains elusive. The aim of the present study was thus to investigate the involvement of autophagy in the pathology of periodontitis. This investigation involved transcriptomic analysis of a broad range of human samples and complemented by in vitro experimentation.

Phase I Trial of Upamostat Combined With Gemcitabine in Locally Unresectable or Metastatic Pancreatic Cancer: Safety and Preliminary Efficacy Assessment.

Aim: This study aimed to determine the maximum tolerated dose (MTD) of the urokinase plasminogen activator (uPA) inhibitor upamostat (LH011) in combination with gemcitabine for locally advanced unresectable or metastatic pancreatic cancer.

Method: Seventeen patients were enrolled and received escalating doses of oral LH011 (100, 200, 400, or 600 mg) daily alongside 1000 mg/m of gemcitabine. Safety profiles, tumor response (including response rate and progression-free survival), pharmacokinetics, and changes in CA199 and D-dimer levels were assessed.