Publications by authors named "Zhi-zhong Qin"
Aim: To study the effect of rosiglitazone, which is a ligand of peroxisome proliferator-activated receptor gamma (PPARgamma), on the expression of PPARgamma in hepatic stellate cells (HSCs) and on the biological characteristics of HSCs.
Methods: The activated HSCs were divided into three groups: control group, 3 micromol/L rosiglitazone group, and 10 micromol/L rosiglitazone group. The expression of PPARgamma, alpha-smooth muscle actin (alpha-SMA), and type I and III collagen was detected by RT-PCR, Western blot and immunocytochemical staining, respectively.
[Expression of subtypes of somatostatin receptors in rat hepatic stellate cells].
Zhonghua Gan Zang Bing Za Zhi
February 2005
Article Synopsis
- The study aimed to explore how interleukin-10 (IL-10) affects the activation of hepatic stellate cells (HSC) via specific cellular pathways.
- HSC were divided into four groups, with varying concentrations of IL-10 administered to three of the groups, and analyses were performed to measure the expression of certain proteins involved in cell signaling.
- Results showed that increased IL-10 levels significantly reduced the expression of proteins linked to cell activation in a dose-dependent manner, indicating that IL-10 inhibits HSC activation through the PDGF/MAPK pathway.
Article Synopsis
- The study aimed to explore how octreotide (OCT), a somatostatin analogue, affects the expression of the CTGF gene in liver cells called hepatic stellate cells (HSCs) in a lab setting.
- OCT was tested on HSCs from rats in various doses to compare gene expression changes with a control group, using RT-PCR to measure results.
- Findings showed that OCT significantly reduces the levels of CTGF and TGF-beta mRNA in HSCs, suggesting its potential as a therapy for preventing liver fibrosis.
Expression of subtypes of somatostatin receptors in hepatic stellate cells.
World J Gastroenterol
June 2004
Aim: To elucidate the mechanism by which somatostatin and its analogue exert the influence on liver fibrosis, and to investigate the mRNA expression of somatostatin receptors subtypes (SSTRs) and the distribution of somatostatin analogue octreotide in rat hepatic stellate cells (HSCs).
Methods: HSCs were isolated from Sprague Dawley (SD) rats by in situ perfusion and density gradient centrifugation. After several passages, the mRNA expression of 5 subtypes of SSTRs were assessed by reverse transcription-polymerase chain reaction (RT-PCR).