[Pathogen Diagnosis of a Febrile HIV Case by the Metagenomic Next-generation Sequencing].

This study is aimed to explore the value of metagenomic next-generation sequencing (mNGS) in diagnosing pathogen in fever patients. It is often a challenge to identify the pathogen that caused the infection in the HIV patients with fever. How could the mNGS be helpful for pathogen diagnosis is unclear.

[Metagenomic Sequencing for Pathogens Detection in a Critically Ill Patient].

Objective: To detect pathogens in a critically ill patient using metagenomic sequencing.

Methods: A critically ill patient with severe acute pancreatitis suffered from abdominal pain and progressed into unconsciousness. Tissue smear, culture, automated biochemical identification and antibiotic susceptibility test, viral load determination by real-time fluorescence quantitative PCR, and immunohistochemical pathological tests were performed to detect pathogens, in addition to metagenomic sequencing based on the BGISEQ-100 high throughput sequencing platform.

Antimicrobial resistance-encoding plasmid clusters with heterogeneous MDR regions driven by IS26 in a single Escherichia coli isolate.

Background: IS26-flanked transposons played an increasingly important part in the mobilization and development of resistance determinants. Heterogeneous resistance-encoding plasmid clusters with polymorphic MDR regions (MRRs) conferred by IS26 in an individual Escherichia coli isolate have not yet been detected.

Objectives: To characterize the complete sequence of a novel blaCTX-M-65- and fosA3-carrying IncZ-7 plasmid with dynamic MRRs from an E.

Comparative genomics of rmtB-carrying IncI1 ST136 plasmids in avian escherichia coli isolates from chickens in China.

Eight rmtB-carrying avian Escherichia coli strains from a farm in China were characterised in our previous study, but little is known about the backbones and entire multiresistance regions (MRRs) of these plasmids. Here, three rmtB-carrying IncI1 ST136 plasmids were analysed by whole-plasmid sequencing and were compared. These plasmids were composed of an 83 470-bp IncI1 backbone carrying genes responsible for plasmid replication, transfer, maintenance and stability functions, as well as a 17 330-bp MRR for pEC006 and pEC007, and a 34 626-bp MRR for pEC008.

Sharp instrument injuries among hospital healthcare workers in mainland China: a cross-sectional study.

Objectives: To determine the prevalence of sharp instrument injuries in hospital-based healthcare workers (HCWs) in mainland China and the contributing factors.

Design: Cross-sectional study.

Setting: The data were derived from public hospitals.

[One Carrier of in China].

Objectives: To study morphological feature, biochemical characteristic and antibiotic resistance of strain.

Methods: The clinical strain was isolated from ananus swab samples being screened in ICU. Biochemical characteristic of the strain was completed by fully automatic microbial identification and drug susceptibility analysis system (BioMérieux, Marcy-l'éE toile, France) with the ANC Vitek2.

Complete Sequence of pEC012, a Multidrug-Resistant IncI1 ST71 Plasmid Carrying bla CTX-M-65, rmtB, fosA3, floR, and oqxAB in an Avian Escherichia coli ST117 Strain.

A 139,622-bp IncI1 ST71 conjugative plasmid pEC012 from an avian Escherichia coli D-ST117 strain was sequenced, which carried five IS26-bracketed resistance modules: IS26-fosA3-orf1-orf2-Δorf3-IS26, IS26-fip-ΔISEcp1-bla CTX-M-65-IS903D-iroN-IS26, IS26-ΔtnpR-bla TEM-1-rmtB-IS26, IS26-oqxAB-IS26, and IS26-floR-aac(3)-IV-IS26. The backbone of pEC012 was similar to that of several other IncI1 ST71 plasmids: pV408, pM105, and pC271, but these plasmids had different arrangements of multidrug resistance region. In addition, the novel ISEc57 element was identified, which is in the IS21 family.

[Nosocomial diarrhea in intensive care unit: other than Clostridium difficile].

Objective: To investigate the incidence and clinical features of non-Clostridium difficile (C. difficile) associated nosocomial diarrhea in intensive care unit (ICU) caused by Klebsiella oxytoca and Clostridium perfringens.

Methods: The faeces of 102 patients with non-C.

[Clonal relatedness of bla(OXA-58)--carrying Acinetobacter baumannii clinical isolates].

Objective: To investigate the clonal relatedness of local bla(OXA-58)-carrying Acinetobacter baumannii clinical isolates.

Methods: Non-duplicated isolates of Acinetobacter baumannii were collected in West China Hospital and verified by recA sequencing. Acquired bla(OXA-58) gene and natural bla(OXA51/66) genes were detected by PCR.

Epidemic plasmid carrying bla(CTX-M-15) in Klebsiella penumoniae in China.

Objective: To investigate the local epidemiology of Klebsiella penumoniae carrying bla(CTX-M-15) in southern China and to characterize the genetic environment of bla(CTX-M-15).

Methods: PCR and DNA sequencing were used to detect and characterize the genetic contexts of bla(CTX-M-15). The clonal relatedness of isolates carrying bla(CTX-M-15) was determined by pulse-field gel electrophoresis.

PCR versus serology for diagnosing Mycoplasma pneumoniae infection: a systematic review & meta-analysis.

Background & Objectives: Diagnosis for Mycoplasma pneumoniae usually relies on serological tests. PCR technology has some advantages but also limitations. The optimal selection for these tests still needs discussion.