[Isolation of rabbit aqueous humor-derived exosomes and their immunosuppression function].

Objective: To isolate exosomes from rabbit aqueous humor and to investigate their immunosuppression function.

Methods: Aqueous humor was collected from 40 New Zealand rabbits and exosomes were isolated by fractional separation and ultracentrifugation methods; the morphology was studied with electron microscopy. The immunosuppressive-related proteins of exosomes were detected with Western blotting; their inhibitory effect on ConA-induced proliferation of T lymphocyte was estimation with CCK-8 cells proliferation assay.

Anticancer, antioxidant and antimicrobial activities of the essential oil of Lycopus lucidus Turcz. var. hirtus Regel.

This study was designed to examine the anticancer, antioxidant and antimicrobial activities of the essential oil from Lycopus lucidus Turcz. var. hirtus Regel.

Composition, antimicrobial activity and cytotoxicity of essential oils from Aristolochia mollissima.

The compositions of the essential oils from the rhizome and the aerial part of Aristolochia mollissima were analysed by GC-MS, 68 constituents (88.2% of the total oil) and 74 constituents (89.4% of the total oil) were identified, respectively.

[Preparation of monoclonal antibodies against human mesenchymal stem cells].

Objective: To prepare monoclonal antibodies (McAbs) against human mesenchymal stem cells (hMSCs) and to study their biological characteristics.

Methods: BALB/C mice were immunized with pooled hMSCs. McAbs were prepared by hybridoma technique and their biological characteristics were analyzed by indirect immunofluorescence, immunohistochemistry and flow cytometry.

[Construction of recombinant plasmid containing mouse vasoactive intestinal peptide gene and its expression in COS-7 cells].

Objective: To construct the eukaryotic expression plasmid containing mouse vasoactive intestinal peptide(VIP) gene with biological activities.

Methods: VIP cDNA including the sequences of signal peptide was cloned from mouse thymus by RT-PCR, and then inserted into the mammalian expression vector pcDNA3.1 between Hind III and EcoR I restriction sites.