Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Alleviate Nuclear Pulposus Cells Degeneration Through the miR-145a-5p/USP31/HIF-1α Signaling Pathway.

Bone marrow mesenchymal stem cell (BMSC)-derived exosomes possess therapeutic potential against degenerative diseases. This study aimed to investigate the effects of BMSC-derived exosomes on intervertebral disc degeneration (IVDD) and explore the underlying molecular mechanisms. Through transcriptome sequencing and histological analysis, we observed a significant increase in HIF-1α expression in degenerative nucleus pulposus (NP) tissues.

Discrimination and prediction of Qingzhuan tea storage year using quantitative chemical profile combined with multivariate analysis: Advantages of MRM based targeted quantification metabolomics.

The duration of storage significantly influences the quality and market value of Qingzhuan tea (QZT). Herein, a high-resolution multiple reaction monitoring (MRM) quantitative method for markers of QZT storage year was developed. Quantitative data alongside multivariate analysis were employed to discriminate and predict the storage year of QZT.

[Analysis of risk factors for readmission in elderly patients with hip fractures undergoing hip hemiarthroplasty].

Objective: To explore the incidence and risk factors of readmission of elderly patients with hip fracture after hip hemiarthroplasty.

Methods: A retrospective analysis of 237 elderly hip fracture patients who underwent hip hemiarthroplasty from February 2015 to October 2020 were performed. According to the readmission status of the patients at 3 months postoperatively, the patients were divided into readmission group (39 cases)and non-readmission group(198 cases).

Earth's youngest banded iron formation implies ferruginous conditions in the Early Cambrian ocean.

It has been proposed that anoxic and iron-rich (ferruginous) marine conditions were common through most of Earth history. This view represents a major shift in our understanding of the evolution of marine chemistry. However, thus far, evidence for ferruginous conditions comes predominantly from Fe-speciation data.

Effects of Pore Size on the Osteoconductivity and Mechanical Properties of Calcium Phosphate Cement in a Rabbit Model.

Calcium phosphate cement (CPC) porous scaffold is widely used as a suitable bone substitute to repair bone defect, but the optimal pore size is unclear yet. The current study aimed to evaluate the effect of different pore sizes on the processing of bone formation in repairing segmental bone defect of rabbits using CPC porous scaffolds. Three kinds of CPC porous scaffolds with 5 mm diameters and 12 mm length were prepared with the same porosity but different pore sizes (Group A: 200-300 µm, Group B: 300-450 µm, Group C: 450-600 µm, respectively).

[Brand-New Ge₂₀Ga₅Sb₁₀S₆₅ Prism Biosensor Based on Inverted SPR].

Based on inverted surface plasmon resonance (ISPR) a novel biosensor consisting of Ge₂₀Ga₅Sb₁₀S₆₅-palladium-graphene layer-biomolecule layer is proposed. The refractive index of biomolecule layer alters as biomolecule experience interactions, thus leading to a shift of ISPR angle. On this basis, the spectrum output of sensor is derived by transfer matrix method.

[Progress and perspective of silkworm as a model of human diseases for drug screening].

As a typical representative of Lepidopteran insects, the silkworm, Bombyx mori, has numerous advantages, such as simple husbandry,highly prolific nature, short generation time, easily handled to be operated with moderate body size, clear genetic background and abundant mutation resources. Silkworm has not only been studied by the geneticists, but also been used as a new laboratory animal model of human disease and drug screening. There is a plenty of genetic resources in silkworm, some of which could be used as models of human genetic diseases, such as Phenylketonuria, Parkinson’s disease, Hermansky-Pudlak syndrome and so on.

Microarray analysis of New Green Cocoon associated genes in silkworm, Bombyx mori.

Green cocoons in silkworm, Bombyx mori, are caused by flavonoids accumulation in the silk proteins, fibroin and sericin. Despite the economic value of natural green cocoon and medical value of flavonoids, there is limited understanding of the molecular mechanism regulating flavonoids uptake in silkworm, which is tightly associated with the trait of green cocoon. The purpose of this study is to perform a comprehensive analysis to understand the molecular mechanisms of flavonoids uptake in silkworm based on microarray analyses.

[A Surface Plasmon Micro-Ring Sensor Suitable for Humidity Sensing].

Temperature is a very important parameter in scientific research, production and life. Almost all the properties of materials are related to temperature. The precise measurement of the temperature is a very important task, so the temperature sensor is widely used as a core part in the temperature measuring instrument.

[A high sensitivity micro-ring humidity sensor based on U-shaped waveguide coupled single micro-ring structure].

The paper proposes a novel high sensitivity humidity sensor based on a U-shaped waveguide-coupled single micro-ring, Polyimide is used as the wet material, the refractive index of humidity-sensing part changes as relative humidity changes, thus leading to a obvious peak drift. The transfer function of the structure is derived basing on transfer matrix method and the paper mainly discusses the influence on the output spectrum with different humidity-sensing parts. Through the theoretical simulation of Matlab, the whole structure of U-shaped waveguide coupled single micro-ring is proved to be the best humidity-sensing part.

[Spectral characteristics of novel accelerometer based on optical microring resonator].

A structure of an optical accelerometer based on an optical single ring resonator located onto the cantilever is proposed to meet the requirements of microminiaturization and economical applications in the present paper. The spectra of out port are obtained using transfer matrix method and theory of coupled modes. According to the measurement of intensity of drop port at the same wavelength, the influences of different structure parameters on the sensitivity of the whole acceleration sensor are analyzed.

[Clinical study of 12 cases with obstetric mirror syndrome].

Objective: To discuss the clinical features, management, pregnancy outcome and prognosis of obstetric mirror syndrome.

Methods: The clinical data of 12 cases with obstetric mirror syndrome at Shenzhen Maternity and Child Healthcare Hospital from April 2008 to December 2010 were collected to retrospectively analyze the clinical features, management, pregnancy outcome and prognosis.

Results: (1) ETIOLOGY: 12 cases with obstetric mirror syndrome included 9 cases of Bart's hydrops fetalis, 2 cases with fetal complicated congenital cardiac anomalies, and 1 case of unknown etiology.

[Construction and identification of recombinant adenovirus vector containing Tet-on adjustable NT3 and BDNF genes].

Aim: To construct Tetracycline(tet) inducible recombinant adenovirus vectors containing human neurotrophin 3(NT3) and brain-derived neurotrophic factor(BDNF) genes, respectively and perform PCR and restrictive enzyme digestion analysis.

Methods: Full length NT3 and BDNF cDNAs were subcloned into pIND vector, followed by being cloned into pTRE-shuttle2 vector. The NT3 and BDNF gene fragments resulted from the pTRE-shuttle2-NT3 and pTRE-shuttle2-BDNF digested with I-Ceu I and PI-Sce I were linked to the linear adeno-X virus DNA.