[Study on ultrasonic extraction and macroporous resin purification techniques of total flavones from Sedum lineare].

Objective: To optimize the ultrasonic extraction condition and purification process of total flavonoids in Sedum lineare by macroporous resin.

Methods: Optimum ultrasonic extraction conditions (ethanol concentration, solid/liquid, ultrasonic time, working temperature) were obtained through orthogonal design. The adsorption ratio and eluting ratio of total flavones were selected as indexes.

[Preparation of anti-P21-activated kinase 5 polyclonal antibody and its application in dental germ cells].

Objective: To clone PAK5-N terminal sequence for expression in E. coli to prepare its polyclonal antibody, and examine the role of PAK5 in dental germ cells.

Methods: Based on human PAK5 cDNA sequence, PCR primers were designed to amplify PAK5-N terminal sequence.

[Effects of cyclosporin A on gene expression profiles of NIT-1 pancreatic beta cell line].

Objective: To observe the effects of cyclosporin A (CsA) on gene expression profiles of NIT-1 pancreatic beta cells cell line using microarray technique.

Methods: NIT-1 cells were exposed to cyclosporin A treatment (10 micromol/L) for 24 h and the differential gene expressions were assessed using microarray technique.

Results: After 24h of CsA treatment, 38 of the 4096 genes tested were up-regulated, including 13 genes with known functions involving stress response, cell growth and protein synthesis.