Chaperone-mediated autophagy protects the bone formation from excessive inflammation through PI3K/AKT/GSK3β/β-catenin pathway.

Multiple regulatory mechanisms are in place to ensure the normal processes of bone metabolism, encompassing both bone formation and absorption. This study has identified chaperone-mediated autophagy (CMA) as a critical regulator that safeguards bone formation from the detrimental effects of excessive inflammation. By silencing LAMP2A or HSCA8, we observed a hindrance in the osteoblast differentiation of human bone marrow mesenchymal stem cells (hBMSCs) in vitro.

[Clinical outcome analysis on using extensor digitorum communis splitting approach for the treatment of radial head fractures].

Objective: To explore the clinical outcomes of internal fixation or replacement for the treatment of radial head fractures through the extensor digitorum communis splitting approach.

Methods: From July 2012 to May 2015, 25 patients with radial head fractures were reviewed. There were 17 males and 8 females, ranging in age from 20 to 67 years old, with a mean age of 39 years old.

A comparison of the clinical effect of two fixation methods on Hoffa fractures.

Introduction: Hoffa fractures are rare and difficult to treat for orthopaedic surgeons. The mechanism of injury of Hoffa fracture is still unknown and the operation approch and fixation method are still controversial. The aim of this study is to compare the clinical effect between two fixation methods on Hoffa fractures.

Experimental study of high-energy fractures delayed operation in promote bone healing.

To investigate role of delayed operation to stimulate growth of strong external callus in high-energy fractures, and explore a new way for bone healing. Twenty adult dogs were employed, and randomly divided into four groups, including group A-D. The dogs underwent osteotomy by wire saw in middle of femur, electric coagulation damaged surrounding periosteum, forming a 1 cm defect.

[Treating Neer two- and three-part of proximal humeral fractures through anterolateral acromial approach and deltopectoral approach].

Objective: To compare clinical results of treating Neer two- and three-part of proximal humeral fractures between anterolateral acromial approach and deltopectoral approach.

Methods: From January 2009 to December 2012, 49 patients with Neer two- and three-part of proximal humeral fractures were treated with locked plate fixation. In anterolateral acromial approach group, there were 22 patients including 9 males and 13 females with an average of (63.

Evolution of the mir-155 family and possible targets in cancers and the immune system.

The mir-155 family is not only involved in a diversity of cancers, but also as a regulator of the immune system. However, the evolutionary history of this family is still unclear. The present study indicates that mir-155 evolved independently with lineage-specific gain of miRNAs.

Morphological characteristics of posterolateral articular fragments in tibial plateau fractures.

Treatment of posterolateral tibial plateau fractures is controversial, and information regarding this specific fracture pattern is lacking. The purpose of this study was to elucidate the frequency and morphological features of posterolateral articular fragments in tibial plateau fractures. A retrospective radiographic and chart review was performed on a consecutive series of patients who sustained tibial plateau fractures between May 2008 and August 2012.

[Cause analysis of 280 case of fractures nonunion].

Objective: To research many clinical data of nonunion cases and discover the reasons for low capacity of bone growth.

Methods: From October 1999 to April 2009,the source material of 280 nonunion cases were conducted and followed up. The data of the study included 230 males and 50 females,with an average age of 39.

Management of hangman's fracture with percutaneous transpedicular screw fixation.

Purpose: This study describes a percutaneous technique for C2 transpedicular screw fixation and evaluates its safety and efficacy in the treatment of patients with hangman's fracture.

Methods: Ten patients with hangman's fracture were treated by percutaneous C2 transpedicular screw fixation. There are six males and four females, who were, based on the classification of Levine and Edwards, sorted as follows: type I fracture, three cases; type II, five cases; type IIa, two cases.

The projection of nerve roots on the posterior aspect of spine from T11 to L5: a cadaver and radiological study.

Study Design: A cadaver and radiological study.

Objective: To investigate the projection of nerve roots on the posterior aspect of the spine from T11 to L5.

Summary Of Background Data: Understanding the projection of nerve roots on the posterior lamina will help to determine the decompressing areas of lamina and avoiding unnecessary bony resection.

Comparison of percutaneous compression plating and short reconstruction nail for treatment of intertrochanteric fracture.

Objective: To compare percutaneous compression plating (PCCP) with Trigen short reconstruction nail (Trigen SRN) for the treatment of intertrochanteric hip fracture.

Methods: Eighty-four patients with intertrochanteric hip fracture admitted to our hospital from January 2007 to June 2008 were included in this retrospective study. Thirty-six patients were treated with PCCP and 48 with Trigen SRN.

[The treatment of knee joint peripheral fractures and/or dislocations with vascular injury].

Objective: To investigate the effect and influence factors on knee joint peripheral fractures and/or dislocations with an associated vascular injury through retrospectively study.

Methods: From March 2002 to November 2007 31 patients with knee joint peripheral fractures and/or dislocations with an associated vascular injury were treated, including 24 males and 7 females with a mean age of 41 years (range from 21 to 62 years). Definite diagnosis of vascular injury by combining colored ultrasonic, CTA, operative exploration with clinical signs, fixing fractures and/or dislocations with fixators, plates and screws, reconstructing blood circulation based on the condition of the vascular injury by vascular repair, homograft vein or artificial vascular grafting separately and analysing the effects of PSI, diagnosis and treatment methods on salvage lower extremities.

Long-term high salt diet causes hypertension and alters renal cytokine gene expression profiles in Sprague-Dawley rats.

Objective: The present study examines whether a long-term high salt diet causes hypertension and renal injury in normal subjects [Sprague-Dawley (SD) rats] and alters renal cytokine-related gene expression profiles.

Methods: Four 10 week old male SD rats received a high salt diet (HS, 8%) and the other 4 SD rats received a normal salt diet (NS, 0.5%) for 8 weeks.

Local lipopolysaccharide injection: a potential novel treatment for heterotopic ossification.

Heterotopic ossification (HO) is a frequent complication after musculoskeletal trauma and surgical procedures. It usually decreases joint mobility and eventually causes loss of joint function. However, there is no satisfied treatment available for HO.

The role of skin and subcutaneous tissues in Dupuytren's contracture: an electron microscopic observation.

Objective: To determine the relationship between the overlying tissues and recurrence of Dupuytren's contracture.

Methods: Forty-three patients (68 hands) who accepted surgical treatment were divided into two groups according to treatment methods: partial fasciectomy or dermofasciectomy and full-thickness skin graft. Diseased palmar fascia, subcutaneous tissues and skin obtained during surgery were then assessed by electron microscopy.

Biomechanical research on anterior double-plate fixation for vertically unstable sacroiliac dislocations.

Objective: To determine the best method for anterior internal fixation by comparing the biomechanical characteristics of various anterior fixation methods for vertically unstable sacroiliac dislocations.

Methods: Eight pelves with Tile C fracture with vertical loading were measured separately. Three- and two-hole anterior double plates were fixed at different angles and compared with sacroiliac screws.

[Stress test and clinical application of the minimal-invasive dynamic hip screw].

Objective: To recognize the effect of minimal-invasive dynamic hip screw (MIDHS) on the treatment of intertrochanteric fracture of the hip by biomechanical test and preliminary clinical application.

Methods: Ten artifical made intertrochanteric fractures of femoral specimen from five cadavers were divided into two groups randomly. The fractures in first group were fixed by MIDHS and other group were fixed by dynamic hip (DHS).

[Treatment of acute and chronic osteomyelitis with negative pressure wound therapy].

Objective: To investigate the treatment of acute and chronic osteomyelitis with negative pressure wound therapy.

Method: Thirty cases of acute and chronic osteomyelitis were treated with negative pressure wound therapy, assisted with debridement, autodermoplasty and myo-cutaneous flap surgery.

Results: No evidence of relapse was found in all cases treated with negative pressure wound therapy.

Ex vivo expansion and transplantation of hematopoietic stem/progenitor cells supported by mesenchymal stem cells from human umbilical cord blood.

Human mesenchymal stem cells (MSCs) are multipotential and are detected in bone marrow (BM), adipose tissue, placenta, and umbilical cord blood (UCB). In this study, we examined the ability of UCB-derived MSCs (UCB-MSCs) to support ex vivo expansion of hematopoietic stem/progenitor cells (HSPCs) from UCB and the engraftment of expanded HSPCs in NOD/SCID mice. The result showed that UCB-MSCs supported the proliferation and differentiation of CD34+ cells in vitro.

[A web-based biomedical image mosaicing system].

This paper describes a web service for biomedical image mosaicing. A web site based on CGI (Common Gateway Interface) is implemented. The system is based on Browser/Server model and is tested in www.

Ex vivo expansion and pluripotential differentiation of cryopreserved human bone marrow mesenchymal stem cells.

This study is aimed at investigating the potentials of ex vivo expansion and pluri-differentiation of cryopreservation of adult human bone marrow mesenchymal stem cells (hMSCs) into chondrocytes, adipocytes and neurocytes. Cryopreserved hMSCs were resuscitated and cultured for 15 passages, and then induced into chondrocytes, adipocytes and neurocytes with corresponding induction medium. The induced cells were observed for morphological properties and detected for expressions of type II collagen, triglyceride or neuron-specific enolase and nestin.

[Preparation of nanometer TiO2 doped with upconversion luminescence agent and investigation on degradation of ethyl violet using visible light].

A novel upconversion luminescence agent 40CdF2 x 60BaF2 x 1.6Er2O3 was synthesized and the fluorescent spectrum was determined. This upconversion luminescence agent can emit five upconversion fluorescent peaks whose wavelengths are all below 387nm under the excitation of 488 nm visible light.

[Tibial infected nonunion treated by internal bone transport using the mono-lateral external fixation].

Objective: To evaluate the clinical and functional outcomes of infected nonunion of the tibia by internal bone transport using the LRS mono-lateral external fixation.

Methods: From June 2003 to October 2005, twenty-eight patients who underwent internal bone transport for infected nonunion in the tibia were reviewed. Twenty-three cases were open fractures: grade IIIA, 5 cases; grade IIIB, 11 cases; grade IIIC, 7 cases.