Two new polyacetylenes from the roots of .

Two new polyacetylenes, (5,12)-14-hydroxytetradeca-5,12-dien-8,10-diyn-1-yl 3-methylbut-2-enoate () and 5, 1'-(6,12)-1-hydroxytetradeca-6,12-dien-8,10-diyn-5-yl 2-methylbutanoate (), together with two known ones, (2,8)-12--methylbutyryltetradeca-2,8-diene-4,6-diyne-1,14-diol (), and ()-5-[5-(but-3-en-1-yn-1-yl)thiophen-2-yl]pent-2-en-4-yn-1-yl acetate () were isolated from the ethyl acetate extract of the roots of by various chromatographic methods, such as normal phase silica gel column, MPLC, and semi-preparative HPLC. Their structures were identified by kinds of spectroscopic methods including 1D NMR, 2D NMR, IR, UV, HR-ESI-MS, and ECD. Compound exhibited cytotoxic activities in HepG2 and Huh7 cells with IC values of 20.

 (Asteraceae), a new species from Xizang (Tibet), China.

, a new species of Asteraceae from the Xizang (Tibet) Autonomous Region of China, is described and illustrated here. Morphologically, it is most similar to but can be distinguished by the combination of monoecious and dioecious individuals, involucral bracts arranged in 3-5 series (with outer series herbaceous and middle to inner series membranous), fimbriate apices on female florets, rough-edged lobes on male florets, and achenes lacking costae. Phylogenetic analyses further support the separation of this new species from related taxa.

Chemical constituents from the roots of .

A new benzofuran-type neolignan (), two new phenylpropanoids ( - ), and one new C steroid () were isolated from the ethyl acetate extract of the roots of by chromatographic methods, including silica gel, ODS column chromatography, MPLC, and semi-preparative HPLC. Their structures were identified as dolosougenin A (), ()-3-isopropylpentyl ()-3-(4-hydroxy-3-methoxyphenyl) acrylate (), ()-3-isopropylpentyl ()-3-(4-hydroxy-3-methoxyphenyl) acrylate () and dolosoucin A () through various spectroscopic techniques including 1D NMR, 2D NMR, IR, UV, HR ESI MS, ORD, and computational ORD methods.

[Two new triterpeneoids from roots of Dolomiaea souliei].

The present study analyzed the chemical constituents in dried roots of Dolomiaea souliei. Chromatographic methods, such as normal-phase, and reversed-phase column chromatography, TLC, and preparative HPLC, were applied to separate and purify the petroleum ether extract of D. souliei.

Lignans from the seeds of Herpetospermum pedunculosum and their farnesoid X receptor-activating effect.

The seeds of Herpetospermum pedunculosum (Ser.) C.B.

Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism.

Background: Cholestasis is characterized by accumulation of bile components in liver and systemic circulation. Restoration of bile acid homeostasis via activating farnesoid x receptor (FXR) is a promising strategy for the treatment of cholestasis. FXR-SHP (small heterodimer partner) axis plays an important role in maintaining bile acid homeostasis.

[One new coumarin from seeds of Herpetospermum pedunculosum].

This paper aims to investigate the chemical constituents of the seeds of Herpetospermum pedunculosum. One new coumarin and two known lignans were isolated from the ethanolic extract of the seeds of H. pedunculosum with thin layer chromatography(TLC), silica gel column chromatography, Sephedax LH-20 chromatography, Semi-preparative high performance liquid chromatography and recrystallization, etc.

The seed oil of ameliorates Aβ25-35-induced Alzheimer's disease in rats.

, a plant of the Paeoniaceae family, has abundant genetic diversity in different populations, and the seed oil can be used in a diverse number of activities. However, its neuroprotective effect is not clear. We investigated the memory-improving effects and associated mechanisms of seed oil (PLSO) on amyloid beta (Aβ)25-35-induced Alzheimer's disease (AD) in rats.

Ptehoosines A and B: Two new sesamin-type sesquilignans with antiangiogenic activity from Pterocephalus hookeri (C.B. Clarke) Höeck.

Two undescribed sesamin-type sesquilignans ptehoosines A (1) and B (2), together with 4 known lignans (3-6), were isolated from Pterocephalus hookeri (C.B. Clarke) Höeck which was widely used as traditional Tibetan medicine for treatment of rheumatoid arthritis.

Dolominol a and B, two new neolignans from (Franch.) C.Shih.

Two new neolignans, dolominol A (1) and dolominol B (2), together with 12 known lignans, -(7,8)-guaiacyl-glycerol--O-4'-dihydroconiferyl ether (3), -(7,​8)-1-​(4-​hydroxy-​3-​methoxyphenyl)​-​2-{4-[()-​3-​hydroxy-​1-​propen​yl)​]-​2-​methoxyphenoxy}-1,​3-​propanediol (4), (-)-dihydrodehydrodiconiferyl alcohol (5), (-)-massoniresinol (6), vladinol D (7), syringaresinol (8), prinsepiol (9), medioresinol (10), (+)-pinoresinol (11), 2-guaicyl-4-oxo-6-catechyl-3,7-dioxabicyclo [3.3.0]octane (12), cycloolivil (13), isolariciresinol (14) were isolated from (Franch.

Berberisides A-D: three novel prenylated benzoic acid derivatives and a clerodane glycoside from aherndt.

Three undescribed prenylated benzoic acid derivatives berberisides A-C () and a new clerodane glycoside berberiside D () were isolated from Aherndt. Their structures were elucidated on the basis of extensive NMR and HR-ESI-MS analysis. The cytotoxic activities of all isolates were studied against lung carcinoma A549, hepatocellular carcinoma HepG2 and breast carcinoma MDA-MB-231 cell lines.

Cytotoxic triterpenoid saponins from .

Two new cycloartane glycosides, cycloatriosides A and B (), and a new oleanolic acid glycoside, thaliatrioside A (), along with known triterpenoids () were isolated from . The structures of the new compounds were established as 3---D-galactopyranosyl (20, 24 )-3,16,25,29-tetrahydroxy-20,24-epoxycycloartane-29-O--D-glucopyranoside (), 3---D-glucopyranosyl-(1→2)--arabinopyranosyl-3,22,30-trihydroxycycloart-24-en-21-oic acid -L-arabinopyranosyl-(1→6)--D-glucopyranoside () and 3--[-L-rhamnopyranosyl-(1→3)--D-xylopyranosyl-(1→3)--L-rhamnopyranosyl-(1→2)--L-arabinopyranosyl]-oleanolic acid 28---D-glucopyranosyl ester () on the basis of extensive NMR and HR-ESI-MS analyses, along with acid hydrolysis. Their cytotoxic activities against human lung cancer cells A549 and human breast cancer cells MDA-MB-231 were evaluated using MTT method.

Correction of Bcl-x splicing improves responses to imatinib in chronic myeloid leukaemia cells and mouse models.

Imatinib mesylate (IM) resistance has become a major clinical problem for chronic myeloid leukaemia (CML). It is known that Bcl-x splicing is deregulated and is involved in multiple malignant cancer initiation and chemotherapy resistance, including CML. The aim of the present study was to correct the abnormal splicing of Bcl-x in CML and investigate the subsequent malignant phenotype changes, especially response to IM.

[Cloning and expression analysis of calmodulin gene from Pinellia ternate].

According to the data of Pinellia ternate transcriptome,two calmodulin genes were cloned and named as Pt Ca M1 and PtCa M2 respectively. The results of bioinformatics analysis showed that Pt Ca Ms genes contained a 450 bp open reading frame,encoding149 amino acids.The identity of the coding sequences was 80%,and the identity of amino acids sequence was 91%.

A new neolignan glycoside from .

One new neolignan glycoside, dolomiside A (), together with 11 known phenylpropanoid glycosides were isolated from (Franch.) Shih. The structures of these isolates were determined by UV, CD, HR-ESI-TOFMS, 1D and 2D NMR analysis.

Bacillamidins A⁻G from a Marine-Derived .

Seven long-chain amides, including five previously undescribed bacillamidins A⁻E (⁻) and two previously reported synthetic analogs, bacillamidins F () and G (), were isolated from extracts of the marine-derived strain RJA1515. The structures of the new compounds were established by extensive analysis of 1D and 2D nuclear magnetic resonance (NMR) data as well as high resolution mass spectrometry (HRMS), and the absolute configurations of the stereogenic carbons of ⁻ were established by comparison of the calculated and the experimental electronic circular dichroism (ECD) spectra. The cytotoxic and antimicrobial activities of ⁻ were evaluated.

[Molecular cloning and characterization of CMK from Artemisia annua].

Artemisinin is a preferred medicine in the treatment of malaria. In this study, AaCMK, a key gene involved in the upstream pathway of artemisinin biosynthesis, was cloned and characterized from Artemisia annua for the first time. The full-length cDNA of AaCMK was 1 462 bp and contained an ORF of 1 197 bp that encoded a 399-anomo-acid polypeptide.

[Effects of nitrogen form on accumulation of alkaloids and expression of relative genes in Atropa belladonna].

Hyoscyamine and scopolamine are two main alkaloids in Atropa belladonna with great medicinal value. In this paper, the contents of hyoscyamine and scopolamine, the upstream products in alkaloid synthesis, and the expression levels of key enzyme genes PMT, TRⅠ and H6H in secondary metabolism of A. belladonna seedlings were measured to clarify the mechanism of nitrogen forms regulating alkaloids synthesis.

[A new napthalenone from roots of Rumex nepalensis].

A new napthalenone, rumexone A (1), was isolated from the roots of Rumex nepalensis. The structure of 1 was elucidated by extensive spectroscopic analyses, including 1D and 2D NMR spectra and MS data. Its cytotoxic effect was evaluated using four clinically relevant human cancer cell lines, gastric carcinoma SGC7901, breast carcinoma MDA-MB-231, lung carcinoma A549, and hepatocellular carcinoma HepG2.

HPLC-ESI-MS/MS analysis of phenolics and in vitro antioxidant activity of Epilobium angustifolium L.

The aerial parts of Epilobium plants are widely used as folk medicine and food around the world. The present study was aimed to investigate the antioxidant activities and active chemical constituents from Epilobium angustifolium L. The results revealed that the EtOAc extract, rich in phenolic compounds and flavonoids (16.

Two new compounds from a marine-derived Streptomyces sp.

Two new long-chain unsaturated compounds, (2E, 6E)-10-methoxy-3,7-dimethyl-10-oxodeca-2,6-dienoic acid (1) and (2E, 6E)-3,7,11-trimethyldodeca-2,6-dienedioic acid (2), together with seven known compounds were isolated from a marine-derived Streptomyces sp. Their structures were determined by spectroscopic methods, including 2D NMR techniques. Compounds 1 and 2 were investigated for their antibacterial activities.

Protective effects of petroleum ether extracts of Herpetospermum caudigerum against α-naphthylisothiocyanate-induced acute cholestasis of rats.

Ethnopharmacological Relevance: The ripe seeds of Herpetospermum caudigerum have been used in Tibetan folk medicine for treatment of bile or liver diseases including jaundice, hepatitis, intumescences or inflammation. Previously reports suggested that the seed oil and some lignans from H. caudigerum exhibited protective effects against carbon tetrachloride (CCl)-induced hepatic damage in rats, which may be related to their free radical scavenging effect.

[Cloning and characterization of an aromatic amino acid aminotransferase(ArAT) gene involved in tropane alkaloid biosynthesis from Hyoscyamus niger].

Tropane alkaloids are anticholinergic drugs widely used clinically. Biosynthesis of tropane alkaloids in planta involves a step of transamination of phenylalanine. Based on the sequenced transcriptomes of lateral roots and leaves of Hyoscyamus niger, we found three annotated aromatic amino acid aminotransferases, which were respectively named HnArAT1, HnArAT2 and HnArAT3.

A new diphenyl ether derivative from Mirabilis himalaica.

One new compound, himalain A (1), together with 12 known compounds were isolated from Mirabilis himalaica. Their structures were determined by spectroscopic methods, including 2D NMR techniques, and the absolute configuration of the 1,2-diol moiety in 1 was defined through Riguera's method. All isolated compounds were tested for their cytotoxic and antibacterial activities.