[Analysis of root development of maxillary inverted impacted central incisor before and after orthodontic traction by CBCT].

Purpose: To investigate the changes of root development before and after orthodontic traction of maxillary inverted impacted central incisors using CBCT and Mimics software.

Methods: Ten patients, who had a maxillary inverted impacted central incisor, were treated using a modified movable retractor combined with surgical eruption. Cone-beam computed tomography(CBCT) was taken before and after treatment.

Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice.

Whole-brain genome editing to correct single-base mutations and reduce or reverse behavioral changes in animal models of autism spectrum disorder (ASD) has not yet been achieved. We developed an apolipoprotein B messenger RNA-editing enzyme, catalytic polypeptide-embedded cytosine base editor (AeCBE) system for converting C·G to T·A base pairs. We demonstrate its effectiveness by targeting AeCBE to an ASD-associated mutation of the MEF2C gene (c.

A Novel Cold-Adapted and High-Alkaline Alginate Lyase with Potential for Alginate Oligosaccharides Preparation.

Alginate oligosaccharides (AOs) prepared through enzymatic reaction by diverse alginate lyases under relatively controllable and moderate conditions possess versatile biological activities. But widely used commercial alginate lyases are still rather rare due to their poor properties (e.g.

[Clinical study of a adjustable movable retractor for maxillary labially inverted impacted central incisor].

Purpose: To evaluate the orthodontic treatment outcome of a new adjustable movable retractor for patients with maxillary labially inverted impacted central incisors.

Methods: Ten patients, aged 7 to 10 years, who had a maxillary labially inverted impacted central incisor, were treated using a new adjustable movable retractor. Cone-beam CT(CBCT) was taken before treatment and after treatment immediately.

Induction of core symptoms of autism spectrum disorder by in vivo CRISPR/Cas9-based gene editing in the brain of adolescent rhesus monkeys.

Although CRISPR/Cas9-mediated gene editing is widely applied to mimic human disorders, whether acute manipulation of disease-causing genes in the brain leads to behavioral abnormalities in non-human primates remains to be determined. Here we induced genetic mutations in MECP2, a critical gene linked to Rett syndrome (RTT) and autism spectrum disorders (ASD), in the hippocampus (DG and CA1-4) of adolescent rhesus monkeys (Macaca mulatta) in vivo via adeno-associated virus (AAV)-delivered Staphylococcus aureus Cas9 with small guide RNAs (sgRNAs) targeting MECP2. In comparison to monkeys injected with AAV-SaCas9 alone (n = 4), numerous autistic-like behavioral abnormalities were identified in the AAV-SaCas9-sgMECP2-injected monkeys (n = 7), including social interaction deficits, abnormal sleep patterns, insensitivity to aversive stimuli, abnormal hand motions, and defective social reward behaviors.

Effect of inappropriate admissions on hospitalization performance in county hospitals: a cross-sectional study in rural China.

Background: Inappropriate admissions cause excessive utilization of health services compared with outpatient services. However, it is still unclear whether inappropriate admissions cause excessive use of health services compared with appropriate admissions. This study aims to clarify the differences in the hospitalization performances between appropriately admitted inpatients and inappropriately admitted inpatients.

Effect of integrated urban and rural residents medical insurance on the utilisation of medical services by residents in China: a propensity score matching with difference-in-differences regression approach.

Objectives: In this study, we aim to evaluate the effect of urban and rural resident medical insurance scheme (URRMI) on the utilisation of medical services by urban and rural residents in the four pilot provinces.

Setting And Participants: The sample used in this study is 13 305 individuals, including 2620 in the treatment group and 10 685 in the control group, from the 2011 and 2015 surveys of China Health and Retirement Longitudinal Study.

Outcome Measures: Propensity score matching and difference-in-differences regression approach (PSM-DID) is used in the study.

siRNA‑mediated knockdown of T‑bet and RORγt contributes to decreased inflammation in pre‑eclampsia.

Abnormal immune response resulting from disordered T helper (Th)1/Th2 and Th17/regulatory T cells (Treg) cytokine expression has been demonstrated to serve an important role in the pathogenesis of preeclampsia (PE). However, the role of transcription factors regulating Th cell differentiation contributing to PE remain unclear. To determine whether a decrease in the expression of the T cell lineage transcription factor T‑bet can restore immune balance and alleviate the systemic inflammatory response present in PE, 30 patients diagnosed with PE were assessed and compared with healthy pregnant controls.

Autism-like behaviours and germline transmission in transgenic monkeys overexpressing MeCP2.

Methyl-CpG binding protein 2 (MeCP2) has crucial roles in transcriptional regulation and microRNA processing. Mutations in the MECP2 gene are found in 90% of patients with Rett syndrome, a severe developmental disorder with autistic phenotypes. Duplications of MECP2-containing genomic segments cause the MECP2 duplication syndrome, which shares core symptoms with autism spectrum disorders.

Vascular endothelial growth factor affects dendritic cell activity in hypertensive disorders of pregnancy.

Vascular endothelial growth factor (VEGF) activity is involved in the growth and stability of the placenta, and its signaling has been implicated in the development of pregnancy‑induced hypertension (PIH). The present study sought to evaluate VEGF levels and dendritic cell (DC) profiles in patients with PIH, and to investigate the effects of VEGF expression on DC phenotypes. The present study assessed 162 patients, 112 of whom were diagnosed with PIH.

Dendritic cells derived from preeclampsia patients influence Th1/Th17 cell differentiation in vitro.

Immune regulation plays important but as-yet-unclear roles in the development of preeclampsia. This study explored potential contributions to immune regulation by dendritic cells (DCs) derived from peripheral blood of preeclampsia patients on the differentiation of Th1 and Th17 cells. Pregnant women with preeclampsia (n = 73) and healthy pregnant women (n = 80) were included in the study.

[Calculating method for crop water requirement based on air temperature].

The importance of accurately estimating crop water requirement for irrigation forecast and agricultural water management has been widely recognized. Although it has been broadly adopted to determine crop evapotranspiration (ETc) via meteorological data and crop coefficient, most of the data in whether forecast are qualitative rather than quantitative except air temperature. Therefore, in this study, how to estimate ETc precisely only using air temperature data in forecast was explored, the accuracy of estimation based on different time scales was also investigated, which was believed to be beneficial to local irrigation forecast as well as optimal management of water and soil resources.

[Temporal and spatial variation of water requirement of winter wheat and its influencing factors in Henan Province, China].

Based on mean meteorological data of ten days in 17 observation stations from 1961 to 2012, the reference crop evapotranspiration was computed using Penman-Monteith formula recommended by FAO. The water requirement of winter wheat in Henan Province was calculated by adopting crop coefficients and the growth stage of winter wheat from the "National Irrigation Experiment Database", and the temporal and spatial distribution, variation and affecting factors in recent 51 years were analyzed by means of time-series analysis and gray relational grade analysis. The results showed that the average water requirement of winter wheat was 345-492 mm in Henan Province from 1961 to 2011, and it was lowest at Lushi station and highest at Mengjin station.

Generation of a monkey with MECP2 mutations by TALEN-based gene targeting.

Gene editing in model organisms has provided critical insights into brain development and diseases. Here, we report the generation of a cynomolgus monkey (Macaca fascicularis) carrying MECP2 mutations using transcription activator-like effector nucleases (TALENs)-mediated gene targeting. After injecting TALENs mRNA into monkey zygotes achieved by in vitro fertilization and embryo transplantation into surrogate monkeys, we obtained one male newborn monkey with an MECP2 deletion caused by frameshifting mutation in various tissues.

[Characteristics of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, East China].

By using Biolog Ecoplate system, this paper studied the structure and functional diversity of soil microbial community under different vegetation types in Wuyishan National Nature Reserve, aimed to probe into the effects of vegetation type on the diversity of soil microbial community. The results showed that the soil chemical properties, soil enzyme activities, and average well color development (AWCD) were higher in natural forest than in planted forest, and were the lowest in abandoned field. The AWCD reflecting soil microbial activity and functional diversity was increased with increasing incubation time, but there existed significant differences among different vegetation types.

Hydrogen sulfide regulates vascular endoplasmic reticulum stress in apolipoprotein E knockout mice.

Background: Atherosclerosis is an important cardiovascular disease, becoming a major and increasing health problem in developed countries. However, the possible underlying mechanisms were not completely clear. In 2009, our research group first discovered that hydrogen sulfide (H(2)S) as a novel gastrotransmitter played an important anti-atherosclerotic role.

The impact of sodium aescinate on acute lung injury induced by oleic acid in rats.

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are associated with high rates of morbidity and mortality. Currently, several surfactant or anti-inflammatory drugs are under test as treatments for ALI. Sodium aescinate (SA) has been shown to exert anti-inflammatory and antiedematous effects.

[Tissue engineered bone scaffold material in restoration of alveolar socket after extraction of lower impacted third molar].

Purpose: To investigate the effects of tissue engineered bone scaffold material in the restoration of alveolar socket after extraction of lower impacted third molar.

Methods: Thirteen patients were immediately implanted with Bio-oss or PerioGlas® in the alveolar cavity after impacted mandibular third molar extraction. Clinical observation and X-ray were taken 1 to 12 weeks after operation.

[Differential expressed genes in eutopic endometrium of endometriosis patients: comparison between Uygur and Han women in Xianjiang].

Objective: To investigate the reason why the incidence of endometriosis (EM) is lower in the Uygur women than in the Han women.

Methods: Eutopic and ectopic endometrium samples were obtained by operation and biopsy from 26 EM patients, 10 Uygur women and 16 Han women and analyzed with a gene expression microarray containing the cDNAs of 22 000 human genes. Twenty-two women, 10 Uygur and 12 Han, were used as controls.

[Supernumerary teeth: report of a rare case and review of the literature].

Supernumerary teeth is a well-recognized clinical phenomenon. Multiple supernumerary teeth are commonly associated with variable syndromes. However the multiple supernumerary teeth reported in mandibular and maxilla premolar region of an 14-year-old female patient without any associated systemic condition/syndrome is a rare case.

[Relationship between endometriosis and glutathione S-transferase M1, T1 genes of the Uygurs and Hans in Xinjiang].

Objective: To examine the association between glutathione S-transferase (GST)M1, T1 null genotypes and endometriosis of the Uygurs and Hans in Xinjiang.

Methods: The polymerase chain reaction method was used to detect the presence or absence of the GSTM1 and GSTT1 genes in genomic DNA from the Uygurs (107 controls and 41 cases) and the Hans (105 controls and 80 cases) in Xinjiang.

Results: The frequencies of the GSTM1-null genotype, GSTT1-null genotype, combined GSTM1-null and GSTT1-null genotype in endometriosis of the Uygurs (51.