Effects of Persimmon Tannin- Composite on Cytotoxic Activities, and Radioprotection Against X-rays Irradiated in Human Hepatoma and Hepatic Cells.

A persimmon tannin- composite powder (PT-A) was investigated for its capacity to protect against ionizing radiation. Human hepatic cells (L02 cells) and human hepatoma cells (HepG2 cells) were pretreated with different concentrations of PT-A or the single compounds (PT or ) and radiated with X-rays. After radiation and post-incubation for 12 h or 24 h, the cell viability, apoptosis, and reactive oxygen species (ROS) production were analyzed by Cell Counting Kit 8 (CCK-8), 2',7'-dichlorfluorescein diacetate (DCFH-DA) staining, and Hoechst 33258 staining/flow cytometry, respectively.

Formulation optimization of chelerythrine loaded O-carboxymethylchitosan microspheres using response surface methodology.

The aims of this investigation were to develop a procedure to prepare chelerythrine (CHE) loaded O-carboxymethylchitosan (O-CMCS) microspheres by emulsion cross-linking method and optimize the process and formulation variables using response surface methodology (RSM) with a three-level, three-factor Box-Behnken design (BBD). The independent variables studied were O-CMCS/CHE ratio, O/W phase ratio, and O-CMCS concentration, dependent variables (responses) were drug loading content and encapsulation efficiency. Mathematical equations and response surface plots were used to relate the dependent and independent variables.

Immobilization of Saccharomyces cerevisiae alcohol dehydrogenase on hybrid alginate-chitosan beads.

Saccharomyces cerevisiae alcohol dehydrogenase (SCAD) was effectively immobilized on hybrid alginate-chitosan beads which are hardened with glutaraldehyde. Immobilization conditions and characterization of the immobilized enzyme were investigated. Orthogonal test design and intuitive analysis method were employed to evaluate the effects of immobilization parameters such as Na-alginate concentration, glutaraldehyde concentration, CaCl(2) concentration and immobilization time.

Stabilizing effect of the transferred conjoined tendon on shoulder stability.

The modified Boytchev procedure, i.e., rerouting of the coracoid process with its attached conjoined tendon (short head of biceps and coracobrachialis) deep to the subscapularis and reattachment to its anatomical location, has been advocated for recurrent anterior glenohumeral instability with controversial clinical outcomes.

[Intraoperative treatment of cyst contents in abdominal echinococcosis].

Objective: To study the methods for intraoperative treatment of the cyst contents in patients with abdominal echinococcosis.

Method: Sixty-nine cases of abdominal echinococcosis were reviewed for intraoperative disposal of the cyst contents using 5% formaldehyde (group A, 33 cases) or 25% NaCl solution (group B, 36 cases), and the effects and incidence of complications were compared.

Results: No relapse occurred in 33 cases with 5% formaldehyde treatment of the cyst contents, but postoperative intestinal fistula took place in 8 cases.

[Cyst puncture aspiration in 40 cases of abdominal echinococcosis].

Objective: To evaluate the efficacy of cyst puncture aspiration in treating abdominal echinococcosis.

Method: A retrospective analysis was performed in 40 cases of abdominal echinococcosis with formation of isolated single cyst in close adhesion with the abdominal wall, for which cyst puncture aspiration was carried out under ultrasound guidance.

Results: No death occurred in these patients and only 15 developed mild fever.