Construction and functional verification of size-reduced plasmids based on TMP resistance gene .

Plasmid size is one of the factors affecting transfection efficacy in most of the molecular genetic research studies. One effective approach for reducing plasmid size is to replace relatively large, conventional antibiotic resistance genes with the short-size gene. The successful construct of a series of based tool plasmids and their functional validation, via comparison with original plasmids, suggest that is a potent drug resistance selection marker.

Active control of terahertz waves based on hybrid VO periodic corrugated waveguides.

We describe a method for the active control of terahertz (THz) waves using hybrid vanadium dioxide (VO) periodic corrugated waveguide. Unlike liquid crystals, graphene and semiconductors and other active materials, VO exhibits a unique insulator-metal transition characteristic by the electric fields, optical, and thermal pumps, resulting in five orders of magnitude changes in its conductivity. Our waveguide consists of two gold coated plates with the VO-embedded periodic grooves, which are placed in parallel with the grooves face to face.

The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper.

Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples.

The complete mitochondrial genome of major malaria vector (Diptera: Culicidae) in China.

(Xu and Feng 1975) is the major vector of malaria in Eastern and Southern China. The species is considered a synonym of (Baisas & Hu, 1936), although they differ in several key biological characteristics. Here, we report the complete mitochondrial genome of for the first time.

Internal Cylinder Identification Based on Different Transmission of Longitudinal and Shear Ultrasonic Waves.

We have built a Fizeau fiber interferometer to investigate the internal cylindrical defects in an aluminum plate based on laser ultrasonic techniques. The ultrasound is excited in the plate by a Q-switched Nd:YAG laser. When the ultrasonic waves interact with the internal defects, the transmitted amplitudes of longitudinal and shear waves are different.

Wave packet interactions in a thin aluminum plate partially immersed in water.

When investigating the wave propagation and mode conversions in a thin aluminum plate partially immersed in water, a kind of wave packet interaction was observed. It was found that the transmitted ultrasonic signal consists of different wave packets, which contain essential information of different wave types. When the incident angle is very small, the signals can be identified as the major wave packet followed by its tail.

The use of facemasks may not lead to an increase in hand-face contact.

Advocacy of the use of facemasks by the public as a measure against the spread of COVID-19 is controversial, with some healthcare professionals arguing that the use of a face mask may increase the rate at which people touch their faces, due to readjusting the mask. We assessed the facial touching behaviour of bus passengers in China before and after the outbreak of COVID-19 and found that wearing a face mask does not increase the number of hand-face contacts and is likely, therefore, to have a positive beneficial effect on suppressing the spread of COVID-19 within populations when used in conjunction with social distancing measures.

Esophageal Scab Mimicking a Parasite: A Case Report.

BACKGROUND Parasitic helminths in the esophagus are rare. Here, we report a case of esophageal scab mimicking a parasite. CASE REPORT A 65-year-old man was admitted to our hospital because after choking on food.

Actin-related protein Arp4 regulates euchromatic gene expression and development through H2A.Z deposition in blood-stage Plasmodium falciparum.

Background: Malaria caused by Plasmodium spp. is still a major threat to public health globally. The various approaches to developing new antimalarial agents rely on the understanding of the complex regulatory mechanisms of dynamic gene expression in the life-cycle of these malaria parasites.

[Inhibition of excretory - secretory antigens on growth of murine Lewis lung carcinoma].

Objective: To investigate the effect of excretory-secretory antigens (ESA) on CD4 CD25 Foxp3 T (Treg) cells in mice carrying Lewis lung carcinoma, and examine the inhibitory effect of ESA on tumor growth.

Methods: C57BL/6 mice were randomly assigned into the PBS group ( = 14) and the Lewis group ( = 34). Mice in the Lewis group were subcutaneously injected with 2 × 105 Lewis lung carcinoma cells in the right axilla, while animals in the PBS group were injected with the same volume of sterile PBS.

Vanadium dioxide-assisted broadband tunable terahertz metamaterial absorber.

Tunable terahertz (THz) functional devices have exhibited superior performances due to the use of active materials, such as liquid crystals, graphene, and semiconductors. However, the tunable range of constitutive parameters of materials is still limited, which leads to the low modulation depth of THz devices. Here, we demonstrate a broadband tunable THz absorber based on hybrid vanadium dioxide (VO) metamaterials.

Acoustic extraordinary transmission manipulation based on proximity effects of heterojunctions.

Heterojunctions between two crystalline semiconductor layers or regions can always lead to engineering the electronic energy bands in various devices, including transistors, solar cells, lasers, and organic electronic devices. The performance of these heterojunction devices depends crucially on the band alignments and their bending at the interfaces, which have been investigated for years according to Anderson's rule, Schottky-Mott rule, Lindhard theory, quantum capacitance, and so on. Here, we demonstrate that by engineering two different acoustic waveguides with forbidden bands, one can achieve an acoustic heterojunction with an extraordinary transmission peak arising in the middle of the former gaps.

[Experience and analysis of excellent works of human parasitology in national medical colleges and universities].

Teaching competition is an effective way for college and university teachers to improve their teaching skills. Based on the teaching practice and experience in medical parasitology, this paper discusses several key issues in teaching competition including topics, teaching designs and teaching methods. It provides references for the teachers in department of parasitology of universities and colleges to improve the quality of classroom teaching.

[Anti-tumor effect of infection on melanoma in mice].

Objective: To explore the anti-tumor effect of 17XL strains of (P.y) infection on melanoma in mice.

Methods: B16F10 tumor cells were axillarilly injected into the right flank of 20 C57BL/6 mice to establish tumor-bearing mouse models.

Single-mode interface states in heterostructure waveguides with Bragg and non-Bragg gaps.

Interface states can always arise in heterostructures that consist of two or more (artificial) materials with topologically different energy bands. The gapped band structure can be classified by the Chern number (a topological invariant) generally or the Zak phase in one-dimensional periodic systems. Recently, topological properties have been employed to investigate the interface states occurring at the connecting regions of the heterostructures of mechanical isostatic lattices and acoustical waveguides.

[Component analysis of excretory/secretory protein from Trichinella spiralis adult worm].

Objective: To analyze the component of adult worm excretory/secretory protein(AWESP) from Trichinella spiralis using the shotgun method, and find out the active component underlying its regulatory effect on colitis in humans.

Methods: The T. spiralis AWESP was prepared, separated by SDS-PAGE, lysed with trypsin, and analyzed by shotgun LC-MS/MS.

[Effect of exogenous nitric oxide on antioxidants from mice infected with ].

Objective: To study the effect of exogenous nitric oxide donor sodium nitroprusside (SNP) on antioxidant enzymes activities and lipid peroxidation of mice infected with .

Methods: BALB/c mice were infected with separated by the digestion method. Forty-two days post-infection, the peripheral blood and hepatic tissue from the infected or normal mice were collected.

[Cloning, expression and identification of gametocyte specific protein Pfgdv1 of Plasmodium falciparum].

Objective: To clone a gametocyte specific protein Pfgdv1 of Plasmodium falciparum, express and identify recombinant Pfgdvl protein in vitro.

Methods: PCR was performed to amplify Pfgdv1 from P. falciparum DNA which was got from the patient who was infected with P.

Vector sequence contamination of the Plasmodium vivax sequence database in PlasmoDB and In silico correction of 26 parasite sequences.

We found a 47 aa protein sequence that occurs 17 times in the Plasmodium vivax nucleotide database published on PlasmoDB. Coding sequence analysis showed multiple restriction enzyme sites within the 141 bp nucleotide sequence, and a His6 tag attached to the 3' end, suggesting cloning vector origins. Sequences with vector contamination were submitted to NCBI, and BLASTN was used to cross-examine whole-genome shotgun contigs (WGS) from four recently deposited P.

Optimized codon usage enhances the expression and immunogenicity of DNA vaccine encoding Taenia solium oncosphere TSOL18 gene.

Cysticercosis due to larval cysts of Taenia solium, is a serious public health problem affecting humans in numerous regions worldwide. The oncospheral stage-specific TSOL18 antigen is a promising candidate for an anti-cysticercosis vaccine. It has been reported that the immunogenicity of the DNA vaccine may be enhanced through codon optimization of candidate genes.

[Immune response of purified Plasmodium falciparum antigen on human peripheral blood T lymphocytes in vitro].

Objective: To explore the effects of the purified Plasmodiumfalciparum (P. f) antigen on the T lymphocytes of the peripheral blood of healthy people in vitro.

Methods: The peripheral blood mononuclear cells (PBMCs) isolated from healthy donators were cultured with purified P.

Orthogonality breaking induces extraordinary single-mode transparency in an elaborate waveguide with wall corrugations.

Orthogonality plays a fundamental role in various mathematical theorems and in physics. The orthogonal eigenfunctions that represent the intrinsic motions of various physical systems can also be regarded as transverse wave modes in a straight waveguide. Because of their orthogonality, these modes propagate independently, without mutual interference.

[Plasmodium vivax specific peptides prediction and screening based on repetitive protein sequences and linear B cell epitope].

Objective: To establish a method based on repetitive protein sequences and linear B cell epitope to predict and screen specific peptides of Plasmodium vivax.

Methods: A P. vivax protein sequence database was reconstructed based on PlasmoDB data, and a customized software for searching of repetitive sequences was used to count the repetition times of each 16 aa peptide in the whole database, and the highly repetitive peptides were chosen to predict the potential linear B cell epitopes.

[Construction and identification of recombinant Mycobacterium smegmatis vaccine expressing Cysticercus cellulosae cC1 antigen].

Objective: To construct recombinant Mycobacterium smegmatis vaccine expressing Cysticercus cellulosae cC1 antigen.

Methods: The recombinant pET28a-cC1 plasmid was extracted and double digested by Xho I and BamH I restriction enzymes, and shuttle plasmid pMV261 was extracted and double digested by Hind III and BamH I restriction enzymes. Both fragments were modified by Klenow fragment to form blunt end, then the large fragments of cC1 and pMV261 plasmid were purified and ligated by T4 ligase enzyme.