Treatment of persistent congenital chylothorax with intrapleural injection of sapylin in infants.

Test the therapeutic efficacy of Sapylin in resolving persistent Congenital Chylothorax (CC) in four infants who failed to respond to conservative medical therapy including Erythromycin and/or Octreotide management. All cases were cured and have no adverse reactions during follow-up. The result shows Sapylin is effective in reducing chylous production.

Meta-analysis of laparoscopic versus open liver resection for colorectal liver metastases.

Background: To compare surgical and oncological outcomes of laparoscopic versus open liver resection for colorectal liver metastases.

Results: A total of 14 retrospective studies with 1679 colorectal liver metastases patients were analyzed: 683 patients treated with laparoscopic liver resection and 996 patients with open liver resection. With respect to surgical outcomes, laparoscopic compared with open liver resection was associated with lower blood loss (MD, -216.

Short interfering RNA directed against the GOLPH3 gene enhances the effect of chemotherapy against oral squamous cell carcinoma by regulating Caspase3, Bcl2 and cytochrome-c expression.

Growing evidence reported that Golgi phosphoprotein 3 (GOLPH3) was involved in the progression of several human cancers. To determine whether knockout of GOLPH3 enhances the effect of Chemotherapy against cell growth of oral squamous cell carcinoma in vitro. OSCC cells were transfected with Golph3 plasmid, Golph3-RNAi and the relative control plasmids.

[The enrichment characteristic and mechanism of gold-silver minerals in submarine hydrothermal sulfides from the ultra-slow-spreading SWIR].

In the present study, content and occurrence of Au, Ag in three submarine hydrothermal sulfide samples from the ultra-slow-spreading Southwest Indian Ridge (SWIR) were studied by using inductively coupled plasma-atomic emission spectrometry (ICP-AES), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The results of ICP-AES show that all of the samples have signs of Au-Ag enrichment. By SEM/EDS, we discovered a mass of gold-silver minerals in the samples.

Hypertension management and lifestyle changes following screening for hypertension in an Asian low socioeconomic status community: a prospective study.

Introduction: This study investigated the effect of an access-enhanced intervention on hypertension screening and management, as well as on health behaviours among newly diagnosed hypertensives, in a multi-ethnic low socioeconomic status (SES) community. Factors associated with hypertension screening, treatment, and control in the community were also determined.

Materials And Methods: The study involved all residents aged ≥40 years in 2 public rental housing precincts (low SES), between 2009 and 2011, who were followed-up prospectively for 1 year after a 6-month community-based intervention comprising a 3-month access-enhanced screening component and a 3-month follow-up (outreach) component.

Purification antibody by thiophilic magnetic sorbent modified with 2-mercapto-1-methylimidazol.

An efficient strategy based on the thiophilic magnetic beads was developed to isolate immunoglobulin G (IgG) from human plasma with high efficiency. Depending on the improved surface modification, the surface density of thiophilic ligand (2-mercapto-1-methylimidazol) reached 1.67mmolg(-1) resulting in a high adsorption capacity (qmax=20.

[Effects of exogenous abscisic acid (ABA) on the photosynthesis and chlorophyll fluorescence parameters of Tripterygium wilfordii seedlings exposed to low temperature].

Taking one year-old Tripterygium wilfordii cutting seedlings as test materials, this paper studied the effects of foliar spraying 0, 5, 10, 15, 20, and 25 mg x L(-1) of abscisic acid (ABA) on the leaf photosynthesis and chlorophyll fluorescence characteristics of the seedlings under low temperature stress. Spraying 20 mg x L(-1) of ABA increased the cold- resistance of the seedlings significantly, manifesting in the slowing down of the decrease amplitudes of leaf net photosynthetic rate (P(n)), transpiration rate (T(r)), stomatal conductance (g(s)), and intercellular CO2 concentration (C(i)) and the increase of photosynthetic capacity. After 6 days exposure to low temperature, the initial fluorescence (F(o)) decreased with increasing concentration of applied ABA, the maximum fluorescence (F(m)) and maximal photochemical yield (F(v)/F(m)) increased, the actual photochemical efficiency of system II (phi(PSII)) and photochemical quenching coefficient (q(P)) increased after an initial decrease, and the non-photochemical quenching coefficient (q(N)) showed a 'decreasing-increasing-decreasing' trend.

Using thiophilic magnetic beads in purification of antibodies from human serum.

The rapid progress of biotechnology, immunology and molecular biology are requiring antibodies with higher purity and stronger activity. Therefore, the development of efficient technology, which is suitable for large-scale purification of antibodies at low cost, becomes much more urgent. In this study, we presented a novel method to isolate immunoglobulin G from human serum based on the utilization of thiophilic magnetic polymer beads.

Efficient isolation of immunoglobulin G by paramagnetic polymer beads modified with 2-mercapto-4-mythyl-pyrimidine.

A potential non-chromatographic technique was explored to purify antibody from human serum based on the thiophilic magnetic beads, which were prepared with vinyl acetate and divinylbenzene by microsuspension polymerization. Depending on the effective surface modification of 2-mercapto-4-mythyl-pyrimidine and divinyl-sulfone, the micron-size magnetic beads could selectively capture immunoglobulin G (IgG) from human serum in a non salt-dependent manner. Just because this specific adsorption towards IgG could be carried out in the physiological pH range (5-7) and at low salt concentration (25 mM PBS aqueous solution), the isolated antibody could keep a high purity (92.

The effective and specific isolation of antibodies from human serum by using thiophilic paramagnetic polymer beads.

A rapid and effective method to specifically isolate immunoglobulin G (IgG) from human serum by thiophilic paramagnetic polymer beads was developed. The thiophilic paramagnetic beads were synthesized with vinyl acetate and divinylbenzene by microsuspension polymerization in the presence of magnetite nanoparticles. Divinylsulfone and 2-mercapto-benzothiazole were subsequently used to modify the surface of these beads, resulting in thiophilic particles that exhibited a high specificity to the antibodies in serum at low salt concentration.

Assessment of released acrosin activity as a measurement of the sperm acrosome reaction.

Aim: To develop a method for assessing sperm function by measuring released acrosin activity during the acrosome reaction (AR).

Methods: Human semen samples were obtained from 24 healthy donors with proven fertility after 3-7 days of sexual abstinence. After collection, samples were liquefied for 30 min at room temperature.

[Expression of vascular endothelial growth factor in dental pulp of immature and mature permanent teeth in human].

Purpose: To investigate the expression and distribution of vascular endothelial growth factor(VEGF) in human dental pulp of immature and mature permanent teeth, and the relationship between VEGF and root development of human permanent teeth.

Methods: The normal teeth extracted for orthodontic or impacted reason were divided into two groups: group 1 contained 10 immature permanent teeth and group 2 contained 15 apical closed mature permanent teeth. The pulps were processed with immunohistochemically staining of VEGF.

[Quantitative analysis of two-component polymer blends (PEG/PE) by infrared spectroscopy].

The concentration of the camponent of PEG/PE blends was analyzed quantitatively by infrared spectroscopy. The absorption peak area ratio of the selected mixture peaks used as the calibrating basis for the quantitative analysis was more reasonable than the peak area ratio of the pure peaks. The theoretical equation deducted by Beer-Lamber law was used to establish the working curve to calculate the composition of the responding functional groups in the film of the PEG/PE blends.

[Biocompatibility of chitosan-carboxymethylchitosan as membrane for periodontal guided tissue regeneration].

Objective: To investigate the biocompatibility of chitosan-carboxymethylchitosan as membrane for periodontal guided tissue regeneration (GTR).

Methods: Chitosan-carboxymethylchitosan blending membrane was prepared by freeze-drying and tests including cytotoxicity test, inserting the membrane into the rabbits, muscles, were employed to investigate its biocompatibility.

Results: Chitosan-carboxymethylchitosan blending membrane showed no toxicity.