[Dynamic Mechanisms of Groundwater Quality by Residual Contaminants of the Tanghe Wastewater Reservoir in Xiong'an New Area].

Tanghe wastewater reservoir(TWR) is located on the west side of Baiyangdian Lake in Xiong'an New Area, where sewage infiltration and irrigation has been taking place for 40 years, and a large number of contaminants have accumulated in the unsaturated zone. Identifying the mechanisms by which this combined system contributes to groundwater hydrochemical dynamics is important for the protection of the water environment in the area. Hydrogeochemical methods such as tracing and improved chlor-alkali index are used to analyze the spatial and temporal characteristics and evolution mechanisms of shallow groundwater.

[Effects of GBE50 on LPS/ATP induced NLRP3 inflammasome activation in primary rat microglia].

In this study, the anti-inflammatory mechanism of Ginkgo biloba extract 50 (GBE50) and its mechanism of action on NLRP3 inflammatory corpuscles were observed by primary microglia cells. LPS/ATP was used to stimulate microglia, and the best time for stimulation and optimal concentration of GBE50 were screened. Pro-inflammatory cytokine IL-1 and TNF-α was determined by ELISA.

[Ginkgo biloba extract 50 inhibited beta-amyloid-induced oxidative stress in rats' hippocampal neurons: an experimental study].

Unlabelled: OBJECTIVE To study the in vitro effect and mechanism of Ginkgo biloba Extract 50 (GBE50) for inhibiting beta-amyloid (Abeta)-induced oxidative stress in rats' hippocampal neurons.

Methods: The primary hippocampal neurons were cultured in vitro and divided into 4 groups, i. e.

GBE50 Attenuates Inflammatory Response by Inhibiting the p38 MAPK and NF- κ B Pathways in LPS-Stimulated Microglial Cells.

Overactivated microglia contribute to a variety of pathological conditions in the central nervous system. The major goal of the present study is to evaluate the potential suppressing effects of a new type of Ginko biloba extract, GBE50, on activated microglia which causes proinflammatory responses and to explore the underlying molecular mechanisms. Murine BV2 microglia cells, with or without pretreatmentof GBE50 at various concentrations, were activated by incubation with lipopolysaccharide (LPS).

Cardio-protection by Ginkgo biloba extract 50 in rats with acute myocardial infarction is related to Na⁺-Ca²⁺ exchanger.

Ginkgo biloba has been used for medical purposes for centuries in traditional Chinese medicine. Ginkgo biloba extract 50 (GBE50) is a new standardized GBE product that matches the standardized German product as EGb761. This paper is aimed at studying the cardio-protection effects of GBE50 Salvia miltiorrhiza on myocardial function, area at risk, myocardial ultra-structure, and expression of calcium handling proteins in rat ischemic myocardium.

Safety and efficacy of nano lamellar TiN coatings on nitinol atrial septal defect occluders in vivo.

Atrial septal defect (ASD) occlusion devices made of nickel-titanium (NiTi) have a major shortcoming in that they release nickel into the body. We modified NiTi occluders using Arc Ion Plating technology. Nano lamellar titanium-nitrogen (TiN) coatings were formed on the surfaces of the occluders.

[Effects of Ginkgo biloba extract 50 on inflammatory cytokines and glia cell ultrastructures in the prefrontal cortex and hippocampus of aging rats].

Objective: To study the effects of Ginkgo biloba extract 50 (GBE50) on inflammatory cytokines and glia cell injury in the prefrontal cortex and hippocampus of aging rats and its probable mechanism. Methods Totally 45 male SD rats were randomly divided into 4 groups, i.e.

[Study on chemical constituents from the root of Hemerocallis citrina].

Objective: To study the chemical constituents root of Hemerocallis citrina.

Methods: The chemical constituents were extracted by microwave method and purified by silica gel column. Their structures were elucidated by their physical and chemical properties and spectral methods.

Three-dimensional synthetic turbulence constructed by spatially randomized fractal interpolation.

A spatially randomized fractal interpolation algorithm to construct three-dimensional synthetic turbulence from original coarse field is reported. As in the one-dimensional case by Ding et al. (Phys.

Subensemble decomposition and Markov process analysis of Burgers turbulence.

A numerical and statistical study is performed to describe the positive and negative local subgrid energy fluxes in the one-dimensional random-force-driven Burgers turbulence (Burgulence). We use a subensemble method to decompose the field into shock wave and rarefaction wave subensembles by group velocity difference. We observe that the shock wave subensemble shows a strong intermittency which dominates the whole Burgulence field, while the rarefaction wave subensemble satisfies the Kolmogorov 1941 (K41) scaling law.

[Effect of GBE50 on delayed rectifier potassium current of ventricular myocytes in ischemic guinea pig].

Objective: Ginkgo biloba extract 50 (GBE50) is a new multicomponent drug with a polyvalent action extracted from the leave of Ginkgo biloba. The aim of this experiment was to study the effects of GBE50 on delayed rectifier potassium current (I(K)) in ventricular myocytes under normal and simulated ischemia conditions in guinea pigs.

Methods: Single ventricular myocytes were isolated by an enzymatic dissociation method.

Synthetic turbulence constructed by spatially randomized fractal interpolation.

A spatially randomized fractal interpolation algorithm to construct synthetic fields with statistical properties close to real turbulence is proposed. It improves the previous works on fractal interpolation so that the position mapping between large and small scales is chosen randomly and the stretching factors are drawn from any chosen random multiplier model. In particular, using different parameters of Log-Poisson model, synthetic fields with absolute scaling properties close to SL94 model (for fully developed turbulence), K41 model and MB2000 model (for magnetohydrodynamic turbulence) are obtained, respectively.

[Effect of precondition with GBE50 and Salviae miltionrrhizae on cycloxygenase-2 and its downstream effectors contents in ischemia/reperfusion myocardium].

Objective: To investigate the changes in contents of cycloxygenase-2 (COX-2) and its downstream effectors in rat's myocardial ischemia/reperfusion (I/R) model and observe the effects of precondition with GBE50 (Ginkgo biloba extract 50) and Salviae miltiorrhizae (SM) on them.

Methods: Rat's I/R model was established by 30-min left anterior descending coronary artery occlusion followed with 60-min reperfusion. Animals were divided into the model control group, the sham-operated group and the tested groups (received 1-week precondition with GBE50 and SM respectively via intragastric infusion before modeling).

[Effects of Ginkgo biloba extract 50 preconditioning on contents of inflammation-related cytokines in myocardium of rats with ischemia-reperfusion injury].

Objective: To investigate the effects of Ginkgo biloba extract 50 (GBE50) preconditioning on contents of inflammation-related cytokines in rats with myocardial ischemia-reperfusion.

Methods: Fifty-eight SD rats were divided into sham-operated group, untreated group, Salviae miltiorrhizae (SM) injection group and low-, medium- and high-dose GBE50 groups. After intragastric administration for 7 d, the left anterior descending (LAD) coronary artery was occluded for 30 min followed by 60-min reperfusion to induce ischemia-reperfusion injury.

[Effect of electroacupuncture combined with Ginkgo biloba extract (GBE 50) on learning-memory ability and hippocampal cytokine levels in rats with dysmnesy].

Objective: To observe the effect of electroacupuncture (EA) combined with Ginkgo biloba extract (GBE 50) on learning-memory ability and hippocampal cytokine contents in aging rats for exploring its underlying mechanism in the treatment of dysmnesy.

Methods: Forty-five SD rats were randomly divided into control (n=9), model (n=8), EA (n=10), GBE 50 (n=9) and EA + GBE 50 (n=9) groups. The dysmnesy model was established by D-galactose intraperitoneal injection for 42 days.

In vitro study of endothelial cells lining vascular grafts grown within the recipient's peritoneal cavity.

A living-tissue conduit with strong mechanical properties was used to produce small-diameter vessels. To improve blood compatibility, a shear-resistant confluent monolayer endothelium was formed on the luminal surface of the conduit. Under mechanical stimulation induced by pulsatile flow in a bioreactor, abrupt high-flow shear stress of 15.

[Effect of electroacupuncture on ethology and cytokines of hippocampus in rats with dysmnesy].

Objective: To observe the effect of electroacupuncture (EA) on learning and memory impairment and cytokines of hippocampus in aging rats induced by D-galactose for exploring its underlying mechanism in the treatment of dysmnesy.

Methods: A total of 27 SD rats were randomly divided into control group (n = 9), model group (n = 8) and EA group (n = 10). Dysmnesy model was induced by intraperitoneal injection of D-galactose.

[Biocompatibility evaluation of chitosan-g-polyvinylpyrrolidone].

Objective: To evaluate the biocompatibility of chitosan-g-polyvinylpyrrolidone as a new scaffold material.

Methods: The material was tested and measured for water absorption and contact angle, followed by evaluation of the biocompatibility by implantation into rabbits and in vitro cultured with the corneal epithelial cells.

Results: The water absorption rate of the material reached 1 100% with contact angle of 83-86.

Effects of Ginkgolide B on action potential and calcium, potassium current in guinea pig ventricular myocytes.

Aim: To investigate the effect of Ginkgolide B (GB) on action potential (AP), delayed rectifier potassium current (IK), and L-type calcium current (I(Ca-L)) in guinea pig ventricular myocytes.

Methods: Single ventricular myocytes were isolated by an enzymatic dissociation method. AP, IK, I(Ca-L) were recorded by whole-cell patch-clamp technique in either current or voltage clamp mode.

[The effect of ginkgolide B on action potential, L-type calcium current and delayed rectifier potassium current in ischemic guinea pig ventricular myocytes].

Aim: To study the effect of ginkgolide B from Ginkgo leave on action potential (AP), L-type calcium current (I(Ca) - L) and delayed rectifier potassium current (I(K)) in normal and ischemic guinea pig ventricular myocytes.

Methods: With the standard microelectrode technique to record action potential and whole-cell variant patch-clamp technique to record calcium and potassium current.

Results: (1) Under normal condition, ginkgolide B shortened APD and had no effect on RP, AP and V(max).

[Cloning and sequencing of chitinase gene from Bacillus thuringiensis subsp israelensis].

Chitin, a linear homopolymer of N-acetyl-D-glucosamine, is a common constituent of fungal cell walls, exoskeletons of insects, and shells of invertebrates. Thus, chitinase, a chitin hydrolytic enzyme, has become of interest for potential use as biopesticides for controlling insect pests. Using a pair of specific primers, chitinase gene (ichi) was amplified by touchdown PCR from Bacillus thuringiensis subsp israelensis chromosomal DNA, and then subcloned into pGEM-T easy vector.

[Analysis of RAPD markers about sterility gene in cytoplasm-nucleolus interacting type rice mitochondrial DNA].

Two specific fragments OPJ18-1000 and OPJ18-1400 were obtained from mitochondrial DNAs of two rice varieties, II-32 and II you 949, by RAPD analysis. The sequenced lengths of the two differential fragments were 1068 bp and 1481 bp, respectively. The result of Southern hybridization demonstrated that the differential fragments were true.

[Effect of ginkgolide B on L-type calcium current and cytosolic [Ca2+]i in guinea pig ischemic ventricular myocytes].

With whole-cell variant patch-clamp and laser scanning confocal microscope technique, we examined the effect of ginkgolide B (GB) from ginkgo leaves on L-type calcium current and cytosolic [Ca(2+)](i) in guinea pig ischemic ventricular myocytes. The results showed that under normal conditions, at a test voltage of 0 mV, GB had no significant effect on I(Ca,L); and during ischemia, the peak Ca(2+) current reduced by 37.71%, and the I-V curve of I(Ca,L) was shifted upward.