Preserving Porcine Genetics: A Simple and Effective Method for On-Site Cryopreservation of Ear Tissue Using Direct Cover Vitrification.

Cell cryopreservation is widely used for porcine genetic conservation; however, isolating and freezing primary cells in farms without adequate experimental equipment and environment poses a significant challenge. Therefore, it is necessary to establish a quick and simple method to freeze tissues on-site, which can be used for deriving primary fibroblasts when needed to achieve porcine genetic conservation. In this study, we explored a suitable approach for porcine ear tissue cryopreservation.

Cytotoxic Nitrobenzoyloxy-substituted Sesquiterpenes from Spongederived Endozoic Fungus Aspergillus insulicola MD10-2.

The emergence of drug resistance and spread of new infectious diseases necessitated the development of novel antibiotics. Marine sponge-associated fungi represent a reservoir of novel molecules with diverse biological potentials. In this study, we isolated five nitrobenzoyloxy-substituted sesquiterpenes 1-5 from the culture mycelia of an endozoic fungus Aspergillus insulicola MD10-2, obtained from the South China Sea sponge Cinachyrella australiensis.

[Optimization of two-dimensional electrophoresis conditions for proteomics of Issatchenkia orientalis in degrading dyes].

Total protein of the yeast Issatchenkia orientalis was extracted and separated by two-dimensional electrophoresis (2-DE) before and after the dye Reactive Brilliant Red K-2BP was degraded by this yeast, respectively. Different protein extraction methods, different volumes of sample loaded and different staining techniques were tested and compared for the 2-DE. Among three different protein extraction methods, the final protein concentrations of 3.

[Decolorization of synthetic dyes with a new isolated yeast Candida krusei].

A new yeast isolate Y-G-1, identified as Candida krusei, capable of degrading Reactive Brilliant Red K-2BP, was obtained from soil by screening experiments. This strain gave a maximal decolorization (99%) for Reactive Brilliant Red K-2BP (200 mg/L) after incubation of 12 h. In order to get this decolorization effect, the optimal inoculation volume of Candida krusei was not less than 5%, the optimal pH of culture medium was within a range from 4 to 9, and the concentrations of (NH4)2SO4 and glucose were not lower than 0.