Additive anticancer effects of chrysin and low dose cisplatin in human malignant glioma cell (U87) proliferation and evaluation of the mechanistic pathway.

Purpose: To evaluate the anticancer effect of chrysin and its additive combination with low-dose cisplatin in human glioma (U87) cancer cells and to study its underlying mechanism.

Methods: Inverted phase and fluorescence microscopic studies were done to demonstrate the effect of chrysin and its combination with cisplatin on cellular morphology and apoptosis. Annexin V-FITC assay was used to quantify the extent of apoptosis in chrysin and chrysin+cisplatin treated cells.

Neural stem cells transplantation alleviate the hyperalgesia of spinal cord injured (SCI) associated with down-regulation of BDNF.

Transplantation of neural stem cells (NSCs) in the injured spinal cord has been shown to improve functional outcome. However, the influence of NSCs transplantation on the sensory function and analgesic behaviors has not been elucidated yet. Here, we investigated whether transplanted NSCs would improve sensory function in rats subjected to complete cord transection (T10) and explore the underlying mechanism.