Depletion of serum-derived exosomes aggravates heat stress-induced damage of bovine mammary epithelial cells.

Background: Exosomes are involved in intercellular communication, affecting many physiological and pathological process. The present study evaluated the effects of serum exosomes on the function of bovine mammary epithelial cells (BMECs) and milk synthesis under heat stress.

Methods And Results: We cultured the BMECs in fetal bovine serum (FBS) or exosome-free FBS medium and examined, their viability using CCK-8 kit.

The protective effect of rosmarinic acid on myotube formation during myoblast differentiation under heat stress.

High ambient temperature is one of the most important environmental factors that caused the reduction of livestock productivity and the increase of mortality. It has been shown that heat stress could affect the meat quality characteristics by physiological and metabolic perturbations in live livestock. Rosmarinic acid (RA) is a natural polyphenolic phytochemical compound that has many important biological activities, such as antioxidant, antimutagenic, and antitumor.

Tissue Levels of Stefin A and Stefin B in Hepatocellular Carcinoma.

Stefins have been reported to be associated with the progression and metastasis of various malignant tumors. However, the expressions of stefins in hepatocellular carcinoma (HCC) have not been well-defined. In this study, the protein levels of stefin A and stefin B were assessed by immunohistochemical staining, and the mRNA levels were quantified by real-time polymerase chain reaction in 85 primary HCC tissues, 85 surrounding non-cancerous tissues, and 9 normal hepatic tissues.

Abnormal DNA methylation in the lumbar spinal cord following chronic constriction injury in rats.

Pathogenesis of neuropathic pain is complex and not clearly understood. Glutamate decarboxylase 67 (GAD 67) is a key synthetic enzyme for the main inhibitory transmitter gamma-aminobutyric acid (GABA), and diminishes in the spinal dorsal horn in rats following chronic constriction injury (CCI). GAD 67 is coded by gene GAD 1.

[Investigation on Clonorchis sinensis infection and its risk factors in Futian District, Shenzhen City].

Objective: To understand the status of Clonorchis sinensis infection and its risk factors in Futian District, Shenzhen City, so as to provide the evidence for formulating the strategy of prevention and control.

Methods: Two monitoring points were randomly selected, and the permanent population aged ≥ 3 years were investigated and their stool samples were detected for the eggs of C. sinensis by using Kato-Katz technique.

The effect of excess Zn on mineral nutrition and antioxidative response in rapeseed seedlings.

Zinc (Zn) is a necessary element for plants, but excess Zn can be detrimental. To investigate Zn toxicity, rapeseed (Brassica napus) seedlings were treated with 0.07-1.

[Distribution and correlation of P and Fe fractions in sediments of typical urban shallow lakes].

The fractions of phosphorus (P) and Fe in the surface sediments of three typical urban shallow lakes (Xuanwu Lake, Daming Lake and Mouchou Lake) were determined using improved methods of SEDEX and BCR, respectively, and the relationship between P and Fe in sediments was also analyzed. P analyses showed that the relative lower content of Al-P was found in sediments of three urban lakes, while the percentage of Fe-P was in the range of 17.10%-27.

[Relation between distribution of phosphorus form in the sediment of typical urban shallow lakes and eutrophication].

The forms of phosphorus in the surface sediments were extracted and determined sequentially with ethylene dinitrilo tetracetic acid (EDTA) technique in three urban shallow lakes: Lake Xuanwu, Lake Mochou and Lake Daming. The results showed that the iron and calcium-bound phosphate, about accounting for 80%, were the main forms of total phosphorus. The contents of iron bound phosphate in Lake Xuanwu and Lake Mochou were higher than that of Lake Daming, reaching 30%-40%.

An efficient and practical synthesis of 2-((1H-indol-3-yl)(aryl)methyl)malononitriles under ultrasound irradiation.

Synthesis of 2-((1H-indol-3-yl)(aryl)methyl)malononitrile via the Michael addition of indole with various arylmethylenemalononitriles was carried out in good yields using anhydrous zinc chloride as the catalyst under ultrasound irradiation.

An improved synthesis of 1,3,5-triaryl-2-pyrazolines in acetic acid aqueous solution under ultrasound irradiation.

Background: Pyrazoline derivatives have been found to possess a broad spectrum of biological activities. Among various pyrazoline derivatives, 2-pyrazolines seem to be the most frequently studied. A variety of methods have been reported for the preparation of this class of compound.

Pinacol coupling of aromatic aldehydes and ketones using TiCl3-Mg under ultrasound irradiation.

Titanium trichloride in EtOH can be reduced by Mg to the corresponding low valent titanium complexes, which can reduce some aromatic aldehydes and ketones to the corresponding pinacols in 8-95% yields within 15-40 min under ultrasound irradiation.

[Determination of the D- and L-configurations of amino deoxy sugars by (S)-TBMB carboxylic acid, a fluorescent chiral derivatization reagent].

D- and L- amino sugars were coupled with (S)-TBMB (S)-TBMB = (S)-2-tert-butyl-2-methyl-1, 3-benzodioxole carbonyl chloride, a fluorescent chiral reagent, followed by per-O-acetylation. The reactions yielded diastereomeric per-O-acetylated N-(S)-TBMB carbonyl amino sugars. Their (1)HNMR signals, especially the strong singlet peaks of tert-Bu and Me groups were diagnostic for the determination of the D-, L- configurations of amino sugar.