[Differentiation of human telomerase reverse transcriptase immortalized human marrow mesenchymal stem cell into chondrocyte].

Objective: To establish an immortalized marrow mesenchymal stem cell line to facilitate advances in cartilage engineering research.

Methods: Human telomerase reverse transcriptase (hTERT) cDNA was transferred into primary human marrow mesenchymal stem cells (hMSC) by retroviral vector pLEGFP-C1-hTERT. Subsequently G418 resistant cell clone was screened and expanded for further studies.

[Arthroscopic assisted diagnosis and treatment of knee extension limitation].

Objective: To figure out the incidence and etiology of knee extension limitation and then to find out the proper methods of arthroscopic assisted diagnosis and treatment.

Methods: We reviewed 303 cases of arthroscopic assisted operation from January to October 2003, 95 cases of which suffered from knee extension limitation before operation, including 54 male and 41female and the mean age was 36.2 years old.