Publications by authors named "Zhi-Kun Wu"

Ardisia crispa(Myrsinaceae) is an ethnomedicine with horticultural and important medicinal values. Its morphology is complex, and its identification is difficult. We analyse the chloroplast genome characteristics and phylogenetic position of A.

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Primula secundiflora is an insect-pollinated, perennial herb belonging to the section Proliferae (Primulaceae) that exhibits considerable variation in its mating system, with predominantly outcrossing populations comprising long-styled and short-styled floral morphs and selfing populations comprising only homostyles. To facilitate future investigations of the population genetics and mating patterns of this species, we developed 25 microsatellite markers from P. secundiflora using next-generation sequencing and measured polymorphism and genetic diversity in a sample of 30 individuals from three natural populations.

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Article Synopsis
  • This study examines the evolutionary shifts from outcrossing to selfing in flowering plants, focusing on the Primula oreodoxa complex.
  • It reveals that these transitions can occur independently in different lineages and that both morphological and genomic changes play a role in this process.
  • The research found that while an older selfing lineage displayed well-defined characteristics of the selfing syndrome, a younger lineage showed parallel development, contradicting expectations that morphological changes would outpace genomic adaptations.
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Article Synopsis
  • This study compares the Sysmex XN9000 (XN-BF) body fluid module with optical microscopy (OM) for analyzing cerebrospinal fluid (CSF) samples using a two-step cell slide centrifuge (TSCSC).
  • Results showed significant differences in red blood cell (RBC) and white blood cell (WBC) counts between TSCSC and one-step centrifuge (OSCSC), with excellent correlation between XN-BF and OM in total cell counts.
  • The study concludes that while XN-BF is effective for RBC and WBC counting, detailed morphological analysis (especially for specific cell types) is still necessary in certain medical conditions, and recommends using the TSCSC procedure for better cell separation.
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from SW Yunnan, China, is described as a species new to science and illustrated. The systematic placement of this new species is also discussed based on an nrITS molecular tree. It is morphologically most similar to , but differs from the latter in its racemose inflorescence, green calyx tube, pink to pink rose corolla, stamens at 1/3 length above the base of the corolla tube and applanate globose capsule.

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Schltr. is a traditional medicine plant in southwestern China. In this study, we characterize the complete chloroplast (cp) genome of based on next-generation sequencing.

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Lindl. is a traditional medicinal plant in southwestern China. In this study, we report the complete chloroplast genome sequence of , using next-generation sequencing technology.

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Background: Glycated hemoglobin (Hb) (HbA) is an indicator that is used to diagnose and monitor the treatment of diabetes. Many factors can affect the detection of HbA. One of the most important of these factors is the Hb variant.

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The evolutionary transition from outcrossing to selfing can have important genomic consequences. Decreased effective population size and the reduced efficacy of selection are predicted to play an important role in the molecular evolution of the genomes of selfing species. We investigated evidence for molecular signatures of the genomic selfing syndrome using 66 species of Primula including distylous (outcrossing) and derived homostylous (selfing) taxa.

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Z.K.Wu & Yuan Huang, a new species of Primulaceae from Dongchuan of northern Yunnan, China, is described and illustrated.

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(Primulaceae) is an endangered endemic herb species in China. In this study, we characterized the complete chloroplast genome of based on next generation sequencing (NGS). The chloroplast genome of was 151,547 bp in size, containing a large single-copy (LSC) region of 82, 662 bp and a small single-copy (SSC) region of 17,749 bp.

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Evolutionary transitions from outcrossing to selfing often occur in heterostylous plants. Selfing homostyles originate within distylous populations and frequently evolve to become reproductively isolated species. We investigated this process in 10 species of Primula section Obconicolisteri using phylogenomic approaches and inferred how often homostyly originated from distyly and its consequences for population genetic diversity and floral trait evolution.

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BACKGROUND Diabetic nephropathy was one of the most serious and harmful diabetic complications, characterized by progressive loss of renal function and renal fibrosis. Aerobic exercise training is an important non-pharmacologic method to prevent and treat diabetes mellitus and diabetic complications. MATERIAL AND METHODS Intraperitoneal (i.

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The breakdown of heterostyly to homostyly is a classic system for the investigation of evolutionary transitions from outcrossing to selfing. Loss of sexual polymorphism is characterized by changes to population morph structure and floral morphology. Here, we used molecular phylogeography to investigate the geographical context for the breakdown process in Primula chungensis, a species with distylous and homostylous populations.

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Twenty-one compounds (nine iridoids and twelve sesquiterpenoids), including ten previously unknown (five iridoids and five sesquiterpenoids) were isolated from whole dried material of Valeriana stenoptera. Structures were established on the basis of extensive spectroscopic analysis and the relative stereochemistry of 13-hydroxypatchoulol A was further confirmed by X-ray crystallographic data. All isolates were evaluated for their effects on nerve growth factor (NGF)-mediated neurite outgrowth in pheochromocytoma (PC12) cells and seven compounds showed potent promoting effects.

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Three new iridoids, jatamanvaltrates R-S (1-2) and jatamanin Q (3), as well as three new sesquiterpenoids, valeriananoids D-E (4, 5) and clovane-2β-isovaleroxy-9α-ol (6), together with nine known compounds were isolated from the roots of Valeriana jatamansi Jones. Compound 2 was the first reported iridoid with fatty acid esters in the Valerianaceae family. The structures of new compounds were established on the basis of extensive spectroscopic analysis.

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Seven new indole alkaloids, rauverines A-G (1-7), and 19 known indole alkaloids were isolated from the leaves and twigs of Rauvolfia verticillata. All compounds showed no cytotoxicity against five human cancer cell lines, human myeloid leukemia (HL-60), hepatocellular carcinoma (SMMC-7721), lung cancer (A-549), breast cancer (MCF-7), and colon cancer (SW480) cells.

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