Correlation between circulating myeloid-derived suppressor cells and Th17 cells in esophageal cancer.

Aim: To perform a comprehensive investigation into the potential correlation between circulating myeloid-derived suppressor cells (MDSCs) and Th17 cells in esophageal cancer (ECA).

Methods: A total of 31 patients newly diagnosed with ECA and 26 healthy subjects were included in the current study. The frequencies of MDSCs and Th17 cells in peripheral blood were determined by flow cytometry.

SRC kinase family inhibitor PP2 promotes DMSO-induced cardiac differentiation of P19 cells and inhibits proliferation.

Background: It has been reported recently that PP2, a Src family kinase inhibitor, promotes selective cardiogenesis in embryonic stem cells. However, there is no other research proved pro-cardiogenic characteristic of PP2 so far. In this study, we explored the potential cardiogenic effect of PP2 on P19 cells differentiation.

[A preliminary study on the effect of lincomycin on the immune function of dendritic cell line DC2.4].

Aim: To explore the effect of lincomycin (lin) on the immune function of dendritic cell (DCs) line DC2.4.

Methods: Three experimental groups, namely, DC2.

[Construction and identification of recombinant adenovirus vector pAdEasy-GFP-GITRL].

Aim: To construct recombinant adenovirus vector pAdEasy-GFP-GITRL and detect the viral titer.

Methods: GITRL gene was obtained by double digestion using Bgl II and Sal I, and cloned into the baculovirus transfer vector(pAdtrack-CMV), then the recombinant adenovirus vector (pAdtrack-CMV-GITRL) was digested by restrictive endoenzyme Pme I. The linear recombinant adenorirus vector and pAdEasy-1 were cotransfected into HEK293 cells by co-precipitate of calcium phosphate.