Baicalein Relieves Ferroptosis-Mediated Phagocytosis Inhibition of Macrophages in Ovarian Endometriosis.

Iron overload and oxidative stress have been reported to contribute to ferroptosis in endometriotic lesions. However, the possible roles of iron overload on macrophages in endometriosis (EMs) remain unknown. Based on recent reports by single-cell sequencing data of endometriosis, here we found significant upregulations of ferroptosis-associated genes in the macrophage of the endometriotic lesion.

[Effect of hypoxia on the activation and visfatin expression in rat hepatic stellate cells].

Objective: To investigate the effect of hypoxia on the visfatin and the expression of smooth muscle-actin (alpha-SMA) and hypoxia-inducible factor-1alpha (HIF-1alpha) in rat hepatic stellate cells (HSCs).

Methods: Rat primary HSCs were isolated from SD rats by in situ perfusion of collagenase and pronase and single-step Nycodenz density gradient centrifugation, and then cultured and activated. Completely activated primary HSCs were exposed to hypoxic conditions (37 degrees C, 5% CO2, 1% O2, 94% N2), or normoxic conditions (37 degrees C, 5% CO2, 21% O2, 74% N2), for 3, 6, 12 or 24 h respectively.

[Clinical features, quality of life and psychological health of patients with irritable bowel syndrome and functional dyspepsia].

Objective: To investigate severity of gastrointestinal symptoms, quality of life, and mental health status of patients with irritable bowel syndrome (IBS) and functional dyspepsia (FD).

Methods: IBS patients were enrolled consecutively at the Outpatient Department of Gastroenterology, West China Hospital, Sichuan University from September 2012 to May 2013. The participants were divided into two groups according to their appearance with FD.

[Comparison of clinical features between gastroesophageal reflux diseases with atypical and typical symptoms].

Objective: To study the differences between gastroesophageal reflux disease (GERD) with atypical symptoms (a-GERD) and typical symptoms (t-GERD).

Methods: 30 patients of suspected a-GERD were recruited and examined with upper gastrointestinal endoscopy, high-resolution manometry (HRM), 24 h esophageal multichannel intra-luminal impedance monitoring with pH sensor (MII-pH) and proton pump inhibitor (PPI) trials. The results were compared with those of 33 cases of GERD with typical symptoms.

[Association between acid reflux and esophageal dysmotility in patients with gastroesophageal reflux disease].

Objective: To investigate the association between esophageal motility and acid reflux in patients with gastroesophageal reflux disease (GERD).

Methods: A total of 94 patients with typical reflux symptoms such as heartburn, regurgitation and chest pain, whose score (Sc) of reflux diagnostic questionnaire (RDQ) was greater than or equal to 12 were enrolled in the study. Each participant was evaluated by upper gastrointestinal endoscopy, high resolution manometry (HRM) of esophagus and 24 h esophageal pH monitoring.

[Advances in the clinical research of gastro-esophageal reflux disease and functional gastrointestinal diseases].

Gastro-esophageal reflux disease (GERD) and functional gastrointestinal diseases (FGIDs) are common digestive diseases with an increasing concern. Both of them share similar pathogenesis, symptoms overlapping and merged mental psychological factors, which cause lack of standardization in diagnosis and poor response to treatment. The topics of this issue focused on the characteristics of motility dysfunction in different subtype of GERD and treatment priorities, main points of diagnosis and treatment of atypical-GERD with extra-esophageal symptoms as the primary presenting manifestation, status and characteristics of symptom overlapping between GERD and FGIDs, as well as the influence of symptom overlapping and mental psychological factors to life quality of GERD and FGIDs patients.

The crosstalk between gut inflammation and gastrointestinal disorders during acute pancreatitis.

The intestinal inflammation caused by intestinal ischemia reperfusion during acute pancreatitis (AP) often leads to multiple organ dysfunction and aggravation of acute pancreatitis. This review concerns up-date progress of the pathophysiology and molecular mechanism of the excessive production of gut-derived cytokines. The regulation effects of immuno-neuro-endocrine network for pancreatic necrosis are the basis for pharmacological therapeutic in AP.

Use of pre-, pro- and synbiotics in patients with acute pancreatitis: a meta-analysis.

Aim: To assess the clinical outcomes of pre-, pro- and synbiotics therapy in patients with acute pancreatitis.

Methods: The databases including Medline, Embase, the Cochrane Library, Web of Science and Chinese Biomedicine Database were searched for all relevant randomized controlled trials that studied the effects of pre-, pro- or synbiotics in patients with acute pancreatitis. Main outcome measures were postoperative infections, pancreatic infections, multiple organ failure (MOF), systemic inflammatory response syndrome (SIRS), length of hospital stay, antibiotic therapy and mortality.

Hydrothermal syntheses and structural characterizations of organic-inorganic hybrid materials of the M(II)-ligand/vanadium oxide system (M(II) = Mn(II), Cu(II) and Zn(II); ligand = 2,2'-bipyridine and 1,10-phenanthroline).

Three organic-inorganic hybrid compounds [Mn(bpy)V(bpy)V(3)O(11)] (bpy = 2,2'-bipyridine) (1), [Cu(bpy)V(2)O(6)] (2) and [Zn(phen)3][V(2)O(6)].10H(2)O (phen = 1,10-phenanthroline) (3) have been synthesized hydrothermally. Single crystal X-ray diffraction analyses revealed that compound 1 is the first example of bpy units coordinating to different transition metals in one molecule.

Combined histology and molecular biology for diagnosis of early stage gastric MALT lymphoma.

Objective: To establish a sequential diagnostic procedure of gastric mucosa-associated lymphoid tissue (MALT) lymphoma and provide evidence for selected optimal cases to be treated in the early stage.

Methods: Thirty-one cases of gastric lymphoid hyperplasia (GLH) were selected and multiple investigations including histology, protein level, DNA and chromosome levels, combined with clinical follow-up were performed. Histological grade was according to Isaacson's criteria of GLH; CD20, UCHL-1 (CD45RO), anti-kappa (kappa), anti-lambda (lambda) and Ki-67 were used for immunohistochemical staining; semi-nested polymerase chain reaction (PCR) was used to detect IgH gene rearrangement and reverse-transcription PCR (RT-PCR) was used to detect API2-MALT1 fusion of the chromosome translocation t(11;18)(q21;q21).

[Expression and function of interleukin-18 in mouse dextran sulfate sodium colitis].

Objective: Interleukin-18 (IL-18) is a proinflammatory cytokine that induces Th1 cytokines production and may play a role in the pathogenesis of inflammatory bowel diseases. This study was aimed at detecting the expression and role of IL-18 in colonic mucosa of dextran sulfate sodium (DSS) colitic mice.

Methods: Expression of IL-18 in colon of DSS colitis was determined by immunohistochemistry.

[The early diagnosis and treatment of gastric mucosa-associated lymphoma].

Objective: To determine the general markers for the diagnosis of gastric mucosa-associated lymphoid tissue (MALT) lymphoma and select optimal cases to be interfered in early stage.

Methods: To investigate the changes of histology, Ki-67 expression, monoclonality of IgH gene rearrangement in gastric lymphoid hyperplasia (GLH) cases before and after Helicobacter pylori (Hp) eradication treatment. Histological changes were classified according to Isaacson's criteria of GLH; L26, UCHL-1, anti-Kappa, anti-Lambda and Ki-67 were applied for immunohistochemical staining; semi-nest PCR was used to detect IgH gene rearrangement.