METTL14 downregulation drives S100A4 monocyte-derived macrophages via MyD88/NF-κB pathway to promote MAFLD progression.

Without intervention, a considerable proportion of patients with metabolism-associated fatty liver disease (MAFLD) will progress from simple steatosis to metabolism-associated steatohepatitis (MASH), liver fibrosis, and even hepatocellular carcinoma. However, the molecular mechanisms that control progressive MAFLD have yet to be fully determined. Here, we unraveled that the expression of the N6-methyladenosine (m6A) methyltransferase METTL14 is remarkably downregulated in the livers of both patients and several murine models of MAFLD, whereas hepatocyte-specific depletion of this methyltransferase aggravated lipid accumulation, liver injury, and fibrosis.

Roles of RNA m6A modification in nonalcoholic fatty liver disease.

NAFLD is a series of liver disorders, and it has become the most prevalent hepatic disease to date. However, there are no approved and effective pharmaceuticals for NAFLD owing to a poor understanding of its pathological mechanisms. While emerging studies have demonstrated that m6A modification is highly associated with NAFLD.

Circular RNA cFAM210A, degradable by HBx, inhibits HCC tumorigenesis by suppressing YBX1 transactivation.

Hepatitis B protein x (HBx) has been reported to promote tumorigenesis in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), but the mechanism awaits further investigation. In this study, we found that cFAM210A (a circular RNA derived from the third exon of transcript NM_001098801 of the FAM210A gene; CircBase ID: hsa_circ_0003979) can be silenced by HBx. cFAM210A expression was downregulated and negatively correlated with tumorigenesis in patients with HBV-related HCC.

Spatial maps of hepatocellular carcinoma transcriptomes reveal spatial expression patterns in tumor immune microenvironment.

Hepatocellular carcinoma (HCC) is a highly heterogeneous and malignant disease with the complex immune microenvironment, which ultimately influence clinic outcomes of patients. However, the spatial expression patterns of diverse immune cells among tumor microenvironment remain to be further deciphered. Spatial transcriptomics sequencing (ST) was implemented on two portions of HCC specimens.

Dysregulated N6-methyladenosine (mA) processing in hepatocellular carcinoma.

N6-methyladenosine (mA) is the most thoroughly studied type of internal RNA modification, as this epigenetic modification is the most abundant in eukaryotic RNAs to date. This modification occurs in various types of RNAs and plays significant roles in dominant RNA-related processes, such as translation, splicing, export and degradation. These processes are catalyzed by three types of prominent enzymes: writers, erasers and readers.

The m6A mRNA demethylase FTO in granulosa cells retards FOS-dependent ovarian aging.

Multifunctional N6-methyladenosine (m6A) has been revealed to be an important epigenetic component in various physiological and pathological processes, but its role in female ovarian aging remains unclear. Thus, we demonstrated m6A demethylase FTO downregulation and the ensuing increased m6A in granulosa cells (GCs) of human aged ovaries, while FTO-knockdown GCs showed faster aging-related phenotypes mediated. Using the m6A-RNA-sequence technique (m6A-seq), increased m6A was found in the FOS-mRNA-3'UTR, which is suggested to be an erasing target of FTO that slows the degradation of FOS-mRNA to upregulate FOS expression in GCs, eventually resulting in GC-mediated ovarian aging.

CD24: a marker of granulosa cell subpopulation and a mediator of ovulation.

Granulosa cells (GCs) play a critical role in driving the formation of ovarian follicles and building the cumulus-oocyte complex surrounding the ovum. We are particularly interested in assessing oocyte quality by examining the detailed gene expression profiles of human cumulus single cells. Using single-cell RNAseq techniques, we extensively investigated the single-cell transcriptomes of the cumulus GC populations from two women with normal ovarian function.

Prediction model for the water jet falling point in fire extinguishing based on a GA-BP neural network.

Past research on the process of extinguishing a fire typically used a traditional linear water jet falling point model and the results ignored external factors, such as environmental conditions and the status of the fire engine, even though the water jet falling point location prediction was often associated with these parameters and showed a nonlinear relationship. This paper constructed a BP (Back Propagation) neural network model. The fire gun nozzle characteristics were included as model inputs, and the water discharge point coordinates were the model outputs; thus, the model could precisely predict the water discharge point with small error and high precision to determine an accurate firing position and allow for the timely adjustment of the spray gun.

The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production.

Background: With the development of RNA-seq technology, tens of thousands of circular RNAs (circRNAs), a novel class of RNAs, have been identified. However, little is known about circRNA formation and biogenesis in hepatocellular carcinoma (HCC).

Methods: We performed ribosomal-depleted RNA-seq profiling of HCC and para-carcinoma tissues and analyzed the expression of a hotspot circRNA derived from the 3'UTR of the stearoyl-CoA desaturase (SCD) gene, termed SCD-circRNA 2.

Role of interleukin-17 in the pathogenesis of perianal abscess and anal fistula: a clinical study on 50 patients with perianal abscess.

Background: To investigate the role of interleukin (IL)-17 in tissue and peripheral blood of perianal abscess and anal fistula.

Methods: Patients with primary perianal abscess (n = 50) admitted to Jinhua Municipal Central Hospital between March 2003 and August 2004 were enrolled. Fifty patients with mixed haemorrhoids, who showed no perianal abscess or anal fistula, were also recruited as the control.

Self-Templated Fabrication of MoNi /MoO Nanorod Arrays with Dual Active Components for Highly Efficient Hydrogen Evolution.

A binder-free efficient MoNi /MoO nanorod array electrode with 3D open structure is developed by using Ni foam as both scaffold and Ni source to form NiMoO precursor, followed by subsequent annealing in a reduction atmosphere. It is discovered that the self-templated conversion of NiMoO into MoNi nanocrystals and MoO as dual active components dramatically boosts the hydrogen evolution reaction (HER) performance. Benefiting from high intrinsic activity, high electrochemical surface area, 3D open network, and improved electron transport, the resulting MoNi /MoO electrode exhibits a remarkable HER activity with extremely low overpotentials of 17 mV at 10 mA cm and 114 mV at 500 mA cm , as well as a superior durability in alkaline medium.

Coupled molybdenum carbide and reduced graphene oxide electrocatalysts for efficient hydrogen evolution.

Electrochemical water splitting is one of the most economical and sustainable methods for large-scale hydrogen production. However, the development of low-cost and earth-abundant non-noble-metal catalysts for the hydrogen evolution reaction remains a challenge. Here we report a two-dimensional coupled hybrid of molybdenum carbide and reduced graphene oxide with a ternary polyoxometalate-polypyrrole/reduced graphene oxide nanocomposite as a precursor.

Self-assembly of a mesoporous ZnS/mediating interface/CdS heterostructure with enhanced visible-light hydrogen-production activity and excellent stability.

We designed and successfully fabricated a ZnS/CdS 3D mesoporous heterostructure with a mediating Zn Cd S interface that serves as a charge carrier transport channel for the first time. The H-production rate and the stability of the heterostructure involving two sulfides were dramatically and simultaneously improved by the careful modification of the interface state a simple post-annealing method. The sample prepared with the optimal parameters exhibited an excellent H-production rate of 106.

Nitrogen-doped Fe/Fe3C@graphitic layer/carbon nanotube hybrids derived from MOFs: efficient bifunctional electrocatalysts for ORR and OER.

A novel nitrogen-doped Fe/Fe3C@graphitic layer/carbon nanotube hybrid derived from MOFs has been first fabricated by a facile approach. The hybrid exhibited outstanding bifunctional electrocatalytic activity for ORR and OER, due to the merits of graphitic layer/carbon nanotube structures with highly active N and Fe/Fe3C sites.

Postoperative initial single fungal discitis progressively spreading to adjacent multiple segments after lumbar discectomy.

Objective: To report multiple cases and investigate etiology of initially single fungal spondylodiscitis that progressively spread to adjacent segments following lumbar discectomy, resulting in multiple spinal involvements.

Methods: From January 2005 to May 2013, ten adult patients were admitted or referred to our institution with postoperative discitis. Fungal infections were confirmed by microbiologic and pathologic examinations.

Heteroatoms ternary-doped porous carbons derived from MOFs as metal-free electrocatalysts for oxygen reduction reaction.

The nitrogen (N), phosphorus (P) and sulphur (S) ternary-doped metal-free porous carbon materials have been successfully synthesized using MOFs as templates (denoted as NPS-C-MOF-5) for oxygen reduction reaction (ORR) for the first time. The influences of porous carbons from carbonizing different MOFs and carbonization temperature on ORR have been systematically investigated. Due to the synergistic effect of N, P and S ternary-doping, the NPS-C-MOF-5 catalyst shows a higher onset potential as a metal-free electrocatalyst for ORR among the currently reported metal-free electrocatalysts, very close to the commercial Pt-C catalyst.

Assessment of mercury erosion by surface water in Wanshan mercury mining area.

Soil erosion is a main cause of land degradation, and in its accelerated form is also one of the most serious ecological environmental problems. Moreover, there are few studies on migration of mercury (Hg) induced by soil erosion in seriously Hg-polluted districts. This paper selected Wanshan Hg mining area, SW China as the study area.