Anterior abdominal abscess - a rare manifestation of severe acute pancreatitis: A case report.

Background: Severe acute pancreatitis (SAP) is a common critical disease of the digestive system. In addition to the clinical manifestations and biochemical changes of acute pancreatitis, SAP is also accompanied by organ failure lasting more than 48 h. SAP is characterized by focal or extensive pancreatic necrosis, hemorrhage and obvious inflammation around the pancreas.

Comparison of short- and long-term outcomes of laparoscopic open resection for gastric gastrointestinal stromal tumors.

Aim: To compare the short- and long-term outcomes of laparoscopic (LR) open resection (OR) for gastric gastrointestinal stromal tumors (gGISTs).

Methods: In total, 301 consecutive patients undergoing LR or OR for pathologically confirmed gGISTs from 2005 to 2014 were enrolled in this retrospective study. After exclusion of 77 patients, 224 eligible patients were enrolled (122 undergoing LR and 102 undergoing OR).

Self-Assembled Polymeric Ionic Liquid-Functionalized Cellulose Nano-crystals: Constructing 3D Ion-conducting Channels Within Ionic Liquid-based Composite Polymer Electrolytes.

Composite polymeric and ionic liquid (IL) electrolytes are some of the most promising electrolyte systems for safer battery technology. Although much effort has been directed towards enhancing the transport properties of polymer electrolytes (PEs) through nanoscopic modification by incorporating nano-fillers, it is still difficult to construct ideal ion conducting networks. Here, a novel class of three-dimensional self-assembled polymeric ionic liquid (PIL)-functionalized cellulose nano-crystals (CNC) confining ILs in surface-grafted PIL polymer chains, able to form colloidal crystal polymer electrolytes (CCPE), is reported.

A simple and controllable graphene-templated approach to synthesise 2D silica-based nanomaterials using water-in-oil microemulsions.

Using the versatility of silica chemistry, we describe herein a simple and controllable approach to synthesise two-dimensional (2D) silica-based nanomaterials: the diversity and utility of the resulting structures offer excellent platforms for many potential applications.

Evaluation of x-inactivation status and cytogenetic stability of human dermal fibroblasts after long-term culture.

Human primary fibroblasts are a popular type of somatic cells for the production of induced pluripotent stem (iPS) cells. Here we characterized biological properties of primary fibroblasts in terms of cell-growth rate, cytogenetic stability, and the number of inactive X chromosomes during long-term passaging. We produced eight lines of female human dermal fibroblasts (HDFs) and found normal karyotype and expected pattern of X chromosome inactivation (XCI) at low passages (Passage P1-5).

[Isolation, culture and cryopreservation of cells derived from fetal appendages].

Objective: To investigate the optimal method for isolation, culture and cryopreservation of cells from fetal appendages, for the purpose of providing viable cells for tissue engineering, cell therapy and gene therapy.

Methods: Trypsin dispersion method was used to isolate cells from human umbilical cord and placenta. The tissues from umbilical cord and placenta were cryopreserved with dimethylsulfoxide (DMSO) in different concentrations.

[Biological characteristics and safety evaluation of endothelial progenitor cells from the umbilical cord blood].

Objective: To investigate the biological characteristics of endothelial progenitor cells (EPCs) from the umbilical cord blood (UCB), and to evaluate their oncogenicity after long-term culture in vitro.

Methods: The mononuclear cells (MNCs) were isolated from the UCB and cultured in MCDB131 medium supplemented with 20% FBS, VEGF and other growth factors. Morphology of the EPCs was observed, and the growth curve of the EPCs was investigated.

[Confirmation of the extra small chromosome in abnormality karyotype by PCR and FISH].

Objective: To investigate the source of the extra small chromosome in a patient with karyotype 45,X[115]/46,X + mar[45]/46,XY[29].

Methods: The SRYgene was detected by PCR, and the chromosome Y probe that labeled with biotin was detected by fluorescence in situ hybridization.

Results: SRY gene is detected positive and the mar chromosome showed positive signal with FISH in human chromosome Y probe pool.

[Biotoxicology and biodynamics of silica nanoparticle].

Objective: To investigate the toxicology and biodynamics of silica nanoparticle.

Methods: The silica nanoparticles were injected into mice through tail vein, and the mice were amphimixised, the urine was collected in different time, variations of pathology in organs and tissues of the mice were detected. At the same time, the silica nanoparticles' distribution in the tissues was observed through electron microscope.

A minidystrophin-EGFP fusion gene expressed in Cos-7 cells mediated by human source vector.

Objective: To construct a human source vector containing minidystrophin-EGFP fusion gene and investigate its expression in Cos-7 cells.

Methods: The recombinant human source vector named pHrnDysG was constructed with PCR-clone methods. Three fragments of dystrophin gene were PCR amplified from normal human dystrophin gene cDNA (GenBank NM04006).

[Silica nanoparticles modified as carriers for gene transfection].

The silica nanoparticles are modified by sodium iodine or sodium chloride with different concentration. Their ability to bind and protect plasmid DNA was demonstrated by agarose gel electrophoresis. The EGFP-N1 plasmid was transfected into HT1080 by combing silica nanoparticle.

Embryonic stem cells generated by nuclear transfer of human somatic nuclei into rabbit oocytes.

To solve the problem of immune incompatibility, nuclear transplantation has been envisaged as a means to produce cells or tissues for human autologous transplantation. Here we have derived embryonic stem cells by the transfer of human somatic nuclei into rabbit oocytes. The number of blastocysts that developed from the fused nuclear transfer was comparable among nuclear donors at ages of 5, 42, 52 and 60 years, and nuclear transfer (NT) embryonic stem cells (ntES cells) were subsequently derived from each of the four age groups.