Publications by authors named "Zhi-Chang He"
Hybridization is an artificial breeding strategy for generating potentially desirable offspring. Recently, a novel Hulong grouper hybrid (Epinephelus fuscogutatus × Epinephelus lanceolatus) yielded significant growth superiority over its parent. Improved innate immunity is considered as another desirable feature during hybridization.
View Article and Find Full Text PDFIdentification and characterization of c-raf from orange-spotted grouper (Epinephelus coioides).
Fish Shellfish Immunol
January 2020
C-Raf proto-oncogene serine/threonine kinase is a mitogen-activated protein kinase (MAP) kinase kinase, which can initiate a mitogen-activated protein kinase (MAPK) cascade by phosphorylating the dual-specific MAP kinase kinases (MEK1/2), and in turn activate the extracellular signal-regulated kinases (ERK1/2). To study the function of c-Raf in teleost fish, a c-Raf cDNA sequence from orange-spotted grouper (Epinephelus coioides) was cloned. Ecc-Raf shared 81%-99% amino acid identity with other vertebrate c-Raf molecules, and shared the highest amino acid identity (99%) with Lates calcarifer c-Raf.
View Article and Find Full Text PDFCharacterization and functional analysis of grouper (Epinephelus coioides) MEK1 and MEK2.
Fish Shellfish Immunol
January 2019
Article Synopsis
- MEK dual-specificity protein kinases are important enzymes that relay signals from outside the cell to the nucleus, and this study focused on characterizing MEK1 and MEK2 in the orange-spotted grouper fish.
- The cloned cDNA sequences showed a high level of amino acid identity (around 80-99%) with both other teleosts and mammals, indicating evolutionary conservation.
- Analysis revealed that EcMEK1 and EcMEK2 are mainly expressed in immune organs, activated certain signaling pathways upon overexpression, and were notably involved in immune responses during infections.