Phenotypic and genomic adaptations to the extremely high elevation in plateau zokor (Myospalax baileyi).

The evolutionary outcomes of high elevation adaptation have been extensively described. However, whether widely distributed high elevation endemic animals adopt uniform mechanisms during adaptation to different elevational environments remains unknown, especially with respect to extreme high elevation environments. To explore this, we analysed the phenotypic and genomic data of seven populations of plateau zokor (Myospalax baileyi) along elevations ranging from 2,700 to 4,300 m.

The distinct role and regulatory mechanism of IL-17 and IFN-γ in the initiation and development of plaque vs guttate psoriasis.

Background: Few studies have explored the differences of immunopathogenesis in plaque vs guttate psoriasis, especially on the inhibitory role of regulatory T cells (Tregs) on IL-17/ IFN-γ production and the impact of CD4+T cells on keratinocytes.

Objective: To investigate the percentage and inhibitory function of CD4+CD25Treg and differential expressions of IL-17/ IFN-γ in plaque vs guttate psoriasis, and the effects of CD4+T cells on the proliferation of keratinocytes.

Methods: Peripheral blood mononuclear cells (PBMCs) were prepared from patients with the plaque and guttate psoriasis.

Bioguided Fraction and Isolation of the Antitumor Components from L.

L., a well-known medicinal plant, has been used as a folk antitumor remedy in the worldwide scale. However, the antitumor components in have not been reported.

[Research on Assessment Methods of Spectrum Data Quality of Core Scan].

Core scan is the instrument used for core spectrum and pictures detection that has been developed in recent years. Cores' data can be digitized with this equipment, then automatic core catalog can be achieved, which provides basis for geological research, mineral deposit study and peripheral deposit prospecting. Meanwhile, an online database of cores can be established by the means of core digitalization to solve the cost problem caused by core preservation and share resources.

Clinical analysis and etiology of porokeratosis.

The present study was performed in order to define the clinical manifestations of porokeratosis, with particular emphasis on genital porokeratosis. A total of 55 cases of porokeratosis were retrospectively reviewed between 2000 and 2007 from Huashan Hospital (Shanghai, China). Out of 55 cases, there were 22 cases of porokeratosis of Mibelli, 17 cases of disseminated superficial actinic porokeratosis (DSAP), 15 cases of disseminated superficial porokeratosis and one case of linear porokeratosis.

Compound K-induced apoptosis of human hepatocellular carcinoma MHCC97-H cells in vitro.

An intestinal bacterial metabolite of ginseng protopanaxadiol saponin, 20-O-(β-D-glucopyranosyl)-20(S)-protopanaxadiol (compound K), has been reported to induce apoptosis in a variety of cancer cells. However, the precise mechanisms induced by compound K in human hepatocellular carcinoma (HCC) cells remain unclear. In order to examine possible apoptotic mechanisms, we investigated the anticancer effect of compound K in MHCC97-H.

Orchid fleck virus: an unclassified bipartite, negative-sense RNA plant virus.

Orchid fleck virus (OFV) causes chlorotic or necrotic spots in many orchid species. Its particle morphology and cytopathic effects are similar to those of nucleorhabdoviruses. Although OFV shares clear sequence similarities with rhabdoviruses, its taxonomic status is undetermined because its negative-sense RNA genome is bipartite.

Dysregulation of miRNA146a versus IRAK1 induces IL-17 persistence in the psoriatic skin lesions.

Psoriasis is a common chronic inflammatory skin disorder with dysregulation of miRNAs. The expression pattern of miR-146a and target gene IRAK1 in lesions and PBMCs of plaque psoriasis remains unclear. In our study, we found the expression of miR-146a was up-regulated both in lesions and PBMCs of psoriatic patients, and positively correlated with IL-17 expression, whereas the target gene IRAK1 expression was expressed differentially in lesions and peripheral blood.

The application of ZnO luminescent nanoparticles in labeling mice.

Photoluminescent ZnO@polymer core-shell nanoparticles were used in mouse imaging through intradermal injections and intravenous injections, and the results proved that such ZnO fluorescence probes are nontoxic to live mice and have great potential in in vivo applications.

In vivo reflectance confocal microscopy of extramammary Paget disease: diagnostic evaluation and surgical management.

Background: Extramammary Paget disease (EMPD) is a diagnostic challenge. In vivo reflectance confocal microscopy (RCM) has been reported to be useful for in vivo skin tumor evaluation. It may also assist in the surgical management of EMPD lesions.

In vivo reflectance confocal microscopy for the differential diagnosis between vitiligo and nevus depigmentosus.

Background: Nevus depigmentosus (ND) is frequently confused with vitiligo. Differential diagnosis can be difficult. In vivo reflectance confocal microscopy (RCM) is a noninvasive technique for real-time en face imaging of the superficial layers of the skin down to the superficial dermis with cellular level resolution close to conventional histopathology.

Suction blister epidermal grafting using a modified suction method in the treatment of stable vitiligo: a retrospective study.

Background: Of various surgical therapies used for the replenishment of melanocytes in recalcitrant and stable vitiligo, suction blister epidermal grafting (SBEG) is one of the simplest and most effective methods.

Objective: To evaluate the effectiveness and potential complications of SBEG in the treatment of stable vitiligo through the use of a modified dermis-epidermis separator designed by the authors.

Materials And Methods: One thousand one hundred people with stable vitiligo unresponsive to other medical treatments were treated with SBEG therapy.

[Wide-range fiber spectral instrument covering].

To meet the requirements of outdoor field mineral analysis and remote sensing ground verification spectral analysis, the wide-range fiber spectral instrument covering 400 nm-2 500 nm was developed. The present article illustrates the design of the optical, mechanical and electrical parts of the instrument. The optical system utilizes grating horizontal-reflecting light route to implement the full coverage of the spectrum.

Type I pityriasis rubra pilaris: upregulation of tumor necrosis factor alpha and response to adalimumab therapy.

Background: pityriasis rubra pilaris (PRP) has unknown etiology and is often refractory to conventional therapies.

Objective: to document a PRP patient's response to adalimumab therapy and to highlight the potential role of tumor necrosis factor (TNF) in the development of PRP skin lesions.

Methods: a patient received adalimumab therapy at standard dosing intervals.

Expression of pigment epithelium-derived factor in human melanocytes and malignant melanoma cells and tissues: Is loss of pigment epithelium-derived factor associated with melanoma?

Background: Pigment epithelium-derived factor (PEDF) was first isolated from the medium conditioned by human fetal retinal pigment epithelial cells and has been detected in a broad range of human fetal and adult tissues. Recent studies have indicated that PEDF activity is inhibitory to angiogenesis.

Objective: To study the expression and distribution of pigment epithelium-derived factor (PEDF) in human melanocytes, malignant melanoma cells and tissues.

[Inhibitory effect of ginseng saponin IH901 on proliferation and metastasis of ECV304 cell line and its molecular mechanism].

This study aims to investigate the inhibitory effect on proliferation and metastasis of 20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol (IH901) on ECV304 cell line. MTT assay was used to examine the effect of cell proliferation inhibition and the adhesive ability of ECV304 cells to artificial basement membrane. Morphology of cell apoptosis was observed with phase contrast microscope.

Reassessment of microarray expression data of porokeratosis by quantitative real-time polymerase chain reaction.

Background: Porokeratosis (PK) is a heterogeneous group of keratinization disorders that exhibit similarities with psoriasis at both the clinical and molecular levels.

Methods: The transcript levels of keratin (KRT) 6A, 16, 17, S100A7, A8, A9, p53 and three candidate genes (i.e.

Evaluation of 308-nm monochromatic excimer light in the treatment of psoriasis vulgaris and palmoplantar psoriasis.

Background: The purpose of this study is to evaluate the efficacy and safety of 308-nm monochromatic excimer light (MEL) in the treatment of psoriasis vulgaris and palmoplantar psoriasis.

Methods: Thirty-five patients with psoriasis vulgaris and 15 patients with palmoplantar psoriasis were recruited for this study. Thirty patients with psoriasis vulgaris completed a total of 16 treatments with 308-nm MEL twice a week, and 15 patients palmoplantar psoriasis completed 25 treatments administered once weekly.

Anti-proliferation and apoptosis induced by a novel intestinal metabolite of ginseng saponin in human hepatocellular carcinoma cells.

20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol (IH-901), a novel intestinal bacterial metabolite of ginseng protopanaxadiol saponins, is reported to induce apoptosis in a variety of cancer cells. We purified the compound and measured its in vitro anti-tumor activity. IH-901 inhibited cell growth of human hepatocellular carcinoma SMMC7721 cells in a dose- and time-dependent manner.

Topical tacalcitol and 308-nm monochromatic excimer light: a synergistic combination for the treatment of vitiligo.

Background/purpose: To study and compare the efficacy of combined 308-nm monochromatic excimer light (MEL) therapy with tacalcitol vs. that of MEL 308-nm therapy alone in treatment of vitiligo.

Methods: Thirty-eight patients with vitiligo were enrolled in a single-blind, within patient controlled clinical trial.

Refinement of DSAP1 locus and mutation detection for candidate genes.

Disseminated superficial actinic porokeratosis (DSAP) is an uncommon autosomal dominant chronic keratinization disorder,characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border. In previous studies,the disease gene was mapped to 12q23. 2-24.

Two closely linked variations in actin cytoskeleton pathway in a Chinese pedigree with disseminated superficial actinic porokeratosis.

Background: Disseminated superficial actinic porokeratosis (DSAP) is an uncommon autosomal dominant chronic keratinization disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border. Recently, SSH1 was identified as the DSAP candidate gene.

Objective: Our purpose was to determine the locus of DSAP and identify the candidate gene(s) of the disease.