Publications by authors named "Zhi Shan Feng"
Article Synopsis
- A study analyzed respiratory infections in over 6,600 patients in Shijiazhuang, China, from January 2021 to December 2023, focusing on the prevalence of various pathogens during and after the COVID-19 pandemic.
- During the pandemic years, nearly one-third of acute respiratory infections were linked to specific pathogens, with a notable increase in positivity rates following the easing of COVID-19 restrictions in 2023.
- The most commonly identified pathogens shifted over the years, with human rhinovirus dominating in 2021, followed by mycoplasma pneumonia in 2022, and influenza A becoming the most prevalent in 2023, particularly among children under 14.
Objective: This study aimed to establish a highly sensitive and rapid single-tube, two-stage, multiplex recombinase-aided qPCR (mRAP) assay to specifically detect the khe, bla, and bla genes in Klebsiella pneumoniae.
Methods: mRAP was carried out in a qPCR instrument within 1 h. The analytical sensitivities of mRAP for khe, bla, and bla genes were tested using recombinant plasmids and dilutions of reference strains.
The most prevalent viruses currently causing diarrhea are norovirus and rotavirus, and rapid and sensitive detection methods are essential for the early diagnosis of disease. The purpose of this study was to establish a sensitive single-tube two-stage nucleic acid amplification method-reverse transcription recombinase-assisted PCR (RT-RAP)-for simultaneous detection of norovirus GII and group A Rotavirus, with the first stage consisting of isothermal reverse transcription recombinase-aided amplification (RT-RAA) and the second stage consisting of qPCR (quantitative PCR). RT-RAP is more sensitive than either RT-RAA or qRT-PCR (quantitative RT-PCR) alone.
View Article and Find Full Text PDFTimely identification of respiratory pathogens guides specific treatment, reduces hospital costs and minimizes the excessive use of antibiotics. A new multiplex real-time PCR panel was developed based on an automatic molecular detection and analysis system (AutoMolec system), consisting of three separate internally controlled assays. Mycoplasma pneumoniae, Chlamydia pneumoniae, adenovirus, human metapneumovirus, influenza B virus, respiratory syncytial virus and human parainfluenza virus 1-3 may be directly detected in original samples.
View Article and Find Full Text PDFEpstein-Barr virus (EBV), a common human γ-herpesvirus, infects more than 90% of adults worldwide. The purpose of this study was to establish a novel EBV detection method by combining the recombinase aided amplification (RAA) assay with an initial enrichment step that utilizes magnetic beads coated with a recombinant human mannan-binding lectin (rhMBL, M1 protein). An M1 protein-protein A magnetic bead complex (M1 beads) was prepared and used to achieve separation and enrichment of EBV from blood.
View Article and Find Full Text PDFVibrio parahaemolyticus (V. parahaemolyticus) is a widely distributed pathogen in the coastal areas, which causes food poisoning and leads to gastroenteritis and sepsis. Therefore, developing a simple, sensitive, and rapid detection method for V.
View Article and Find Full Text PDFEpstein-Barr virus (EBV) and cytomegalovirus (CMV), two of the most prevalent human herpesviruses, cause a wide spectrum of diseases and symptoms and are associated with serious health problem. In this study, we developed an internal control reference recombinase-aided amplification (ICR-RAA) assay for the rapid detection of EBV and CMV within 30 min. The assay had a sensitivity of 5 and 1 copies/test for EBV and CMV, respectively, with no cross reaction with other pathogens.
View Article and Find Full Text PDFObjectives: Bordetella pertussis is a highly contagious respiratory agent and is the causative pathogen of pertussis, which primarily affects children. Current diagnostic techniques for this pathogen have a variety of limitations including a long culture time, low bacterial load, and lack of specificity.
Methods: This article reports the development of a one-tube nested quantitative real-time PCR assay using the locked nucleic acid (LNA) technique (LNA-OTN-q-PCR), targeting the BP485 gene and using a simple inexpensive extraction method.
Multiplex real-time quantitative polymerase chain reaction (mRT-qPCR) assay is commonly used to detect respiratory viruses, however, the sensitivity is limited for most reports. A panel of locked nucleic acid based multiplex closed one-tube nested real-time PCR (mOTNRT-PCR) assay consisting of five separate internally controlled RT-qPCR assays was developed for detection of 14 respiratory viruses. The sensitivity and reproducibility of mOTNRT-PCR panel were evaluated using plasmid standards and the specificity was evaluated using clinical samples.
View Article and Find Full Text PDFBackground: Human adenovirus (HAdV) is a common pathogen in children that can cause acute respiratory tract infection (ARTI), but the molecular epidemiological and clinical information relating to HAdV among hospitalized children with ARTI are few reported in China.
Objectives: To evaluate the epidemiological, clinical, and molecular characteristics of HAdV infections among hospitalized children with ARTI in Hebei, Northern China from June 2017 to May 2018.
Study Design: A 12-month longitudinal, retrospective study on HAdV, typed by nested polymerase chain reaction targeting the hexon gene's hypervariable region (typing was merely performed by sequencing of the hexon neutralization epitope and thus genotypes could not be identified unequivocally), associated with ARTI was performed.
Article Synopsis
- A study of 4,148 hospitalized children with community-acquired pneumonia (CAP) revealed that 38.18% of those with Mycoplasma pneumoniae (Mp) were also co-detected with other pathogens, notably more in younger children (≤3 years).
- Most clinical symptoms and disease severity showed no significant differences between children with Mp alone and those with co-detection, although some specific symptoms like prolonged fever and runny nose were more common in those with Mp-viral co-detection.
- Findings indicate that while Mp co-detections with other pathogens are frequent, they generally do not alter the clinical presentation or severity of the illness.
Objectives: Pertussis is a highly transmissible acute respiratory infection caused by the bacterial pathogen Bordetella pertussis. The purpose of this study was to develop a rapid, simple and sensitive diagnostic test for detecting this pathogen.
Methods: Here we present a recombinase aided amplification (RAA) assay incorporating competitive internal amplification control (IAC) to detect Bordetella pertussis using the DNA obtained by boiling.
Background: Hepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.
Methods: Here we present a rapid and sensitive recombinase aided amplification assay (RAA) to detect HBV at 39.
Article Synopsis
- RSV, HRV, and HMPV are key viruses that cause respiratory infections in hospitalized patients, making accurate detection crucial for treatment.
- A new test called mOTNRT-PCR was developed, which can simultaneously detect these viruses with high sensitivity (5 copies/reaction) and no cross-reactivity with other viruses.
- The mOTNRT-PCR outperformed traditional RT-qPCR methods, identifying more positive cases and confirming 33 samples that were missed by RT-qPCR through sequencing.
Article Synopsis
- Respiratory syncytial virus (RSV) is a major cause of respiratory infections, particularly challenging to detect in vulnerable populations due to its low viral load.
- Researchers developed a highly sensitive assay called OTNRT-PCR that is easier to use and reduces contamination risks compared to traditional methods.
- The study found that OTNRT-PCR detects RSV more accurately than conventional qRT-PCR, confirming 143 samples that were previously considered negative.
Some serotypes of enterovirus (EV) may lead to transient and symptomatic gastrointestinal infections while others are commensal residents of the human gut. To determine whether certain EV types are more often associated with diarrhea, we conducted a preliminary study on the prevalence of EV serotypes and common diarrhea viruses in fecal samples of diarrhea children and healthy controls. EV was tested with one step nest polymerase chain reaction and typed by direct sequencing while common causative diarrhea viruses rotavirus (RV), norovirus (NoV), adenovirus (AdV), bocavirus (HBoV), and astrovirus (AstV) were screened with multiplex PCR assays.
View Article and Find Full Text PDFBackground: Diarrhea is a major source of morbidity and mortality among young children in low-income and middle-income countries. Human adenoviruses (HAdV), particular HAdV species F (40, 41) has been recognized as important causal pathogens, however limited data exist on molecular epidemiology of other HAdV associated with acute gastroenteritis.
Methods: In the present preliminary study, we performed a case-control study involving 273 children who presented diarrheal disease and 361 healthy children matched control in Children's hospital of Hebei Province (China) to investigate the relationship between non-enteric HAdV and diarrhea.
Background: Human adenovirus (HAdV) serotypes 2, 3 and 7 are more prevalent than other serotypes and have been associated with severe pneumonia in pediatric children. Molecular typing of HAdV is not routinely performed in clinical diagnostic laboratories as it is time-consuming and labor-intensive.
Methods: In the present study, we developed a triplex quantitative real-time PCR assay (tq-PCR) in a single closed tube for differential detection and quantitative analysis of HAdV serotypes 2, 3 and 7.
The sensitivity of qRT-PCR assay is not adequate for the detection of the samples with lower viral load, particularly in the cerebrospinal fluid (CSF) of patients. Here, we present the development of a highly sensitive real-time nested RT-PCR (RTN RT-PCR) assay in a single closed tube for detection of human enterovirus (HEV). The clinical performance of both RTN RT-PCR and qRT-PCR was also tested and compared using 140 CSF and fecal specimens.
View Article and Find Full Text PDFUse of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection.
Diagn Microbiol Infect Dis
February 2018
In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39°C in less than 30min. The analytical sensitivities of RSVA and RSVB at 95% probability by probit regression analysis were 38copies per reaction and 35 copies per reaction, respectively, and no cross reactions with other related respiratory viruses were observed.
View Article and Find Full Text PDFArticle Synopsis
- The Respiratory Pathogen 13 Detection Kit (13× kit) detects 11 respiratory viruses along with Mycoplasma pneumoniae and Chlamydia in one reaction.
- In a study of 572 nasopharyngeal samples from hospitalized children, the 13× kit demonstrated a high accuracy rate of 95.98% when compared to a standard 2-tube multiplex PCR assay.
- The kit's effectiveness in diagnosing MP and Chlamydia can assist doctors in making informed treatment decisions, potentially reducing unnecessary antibiotic prescriptions.
[Effect of baicalin on expression of heme oxygenase-1 in lung injury of rats associated with paraquat poisoning].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
June 2006
Objective: To investigate the effect of baicalin (Bai) on lung injury, the level of TNF-alpha in cultured liquid of pulmonary interstitial macrophage and the expression of heme oxygenase-1 (HO-1) in lung injury associated with paraquat poisoning.
Methods: Rats were randomizedly divided into four groups: control group, PQ group, Bai group (Bai, 300 mg.kg(-1).