Morphology, phylogeny, and mitogenomics reveal a new entomopathogenic fungus, (Hypocreales, Clavicipitaceae), from southwestern China.

Two species of (Clavicipitaceae, Hypocreales, Ascomycota) were discovered during an investigation of the diversity of entomopathogenic fungi. A new fungus and one known fungal species that were gathered from Yunnan Province were described in this study. , sp.

(Hypocreales, Clavicipitaceae): a new species from Yunnan, south-western China.

Background: As a genus within the Clavicipitaceae, exhibits rich morphological and ecological diversity, with a wide distribution and a variety of hosts. Currently, sixty-eight species of have been described.

New Information: A new species of , (Hong Yu bis), was described in Pu'er City, Yunnan Province, south-western China.

Multi-locus molecular phylogenetic analysis reveals two new species of (Bionectriaceae, Hypocreales).

has been previously accepted as a member of the Cordycipitaceae and currently it is considered a member of the Bionectriaceae. The substrates of were complex and varied, being mainly animal faeces. This study reports two new species of from Yunnan Province in south-western China.

Morphological and Phylogenetic Analyses Reveal Three New Species of Entomopathogenic Fungi Belonging to Clavicipitaceae (Hypocreales, Ascomycota).

This study aims to report three new species of and from Yunnan Province, Southwestern China. Species of and parasitize scale insects (Coccidae and Lecaniidae, Hemiptera) and whiteflies (Aleyrodidae, Hemiptera). Based on the phylogenetic analyses of the three-gene nrLSU, , and , it showed one new record species () and one new species ( sp.

Species diversity and major host/substrate associations of the genus (Hypocreales, Cordycipitaceae).

, a group of fungi with rich morphological and ecological diversity in Cordycipitaceae (Ascomycota, Hypocreales), has a wide distribution amongst diverse habitats. By surveying arthropod-pathogenic fungi in China and Southeast Asia over the last six years, nine spp. were found and identified.

Phylogeny and species delimitations in the economically, medically, and ecologically important genus (Cordycipitaceae, Hypocreales).

is a ubiquitous genus of cosmopolitan arthropod-pathogenic fungi in the family Cordycipitaceae. The fungi have economic, medicinal, and ecological importance. Prior taxonomic studies of these fungi relied predominantly on phylogenetic inferences from five loci, namely, the nuclear ribosomal small and large subunits (nr SSU and nr LSU), the 3' portion of translation elongation factor 1 alpha (3P_), and RNA polymerase II subunits 1 and 2 ( and ).

Phylogeny and Systematics of the Genus (Ophiocordycipitaceae, Hypocreales).

The taxonomy and phylogeny of the genus are herein revised based on the most comprehensive dataset to date. Two species-level phylogenies of were constructed: a single-gene phylogeny (ITS) of 35 accepted species and a multigene phylogeny (nr, nr, -, and ) of 27 accepted species. Three new species, sp.

[Effect of moxibustion on Nogo/neurotrophin receptor signaling pathway in rats with cerebral ischemia/reperfusion injury].

Objective: To observe the effect of moxibustion treatment on the expression of Nogo-A, Nogo receptor (NgR), neurotrophin receptor p75 (p75NTR) and leucine rich repeat and Ig domain containing 1 (Lingo-1) in brain tissue of rats with cerebral ischemia/reperfusion injury (CI/RI), so as to analyze its mechanism underlying improvement of CI/RI.

Methods: Male SD rats were randomly divided into sham operation group (16 rats), model group (17 rats), NEP1-40 (extracellular peptide residues 1-40, a blocker targeting NgR) group (model+blocker, 17 rats) and moxibustion group (model+moxibustion, 17 rats). The CI/RI model was established by occlusion of the left middle cerebral artery (MCAO).

Morphology and Phylogeny Reveal Five Novel Species in the Genus (Cordycipitaceae, Hypocreales) From Yunnan, China.

The current study was aimed to introduce five new species of from Yunnan, with morphological descriptions, illustrations, color photographs, phylogenetic placement, associated host, and a comparison with allied taxa. The five new species were morphologically distinct from all other species, and it was also suggested that they should differ from other species in the genus based on combined multigene analyses. Employing DNA nucleotide sequences of the nr, nr, α, , and , the five new species were recognized in the clade of by using molecular phylogenetic analyses, including five well-supported subclades: three new species, , and , were found in the subclade of , and two new species, and , were located in the subclade of .

Octanuclear Cobalt(II) Cluster-Based Metal-Organic Framework with Caged Structure Exhibiting the Selective Adsorption of Ethane over Ethylene.

A novel metal-organic framework (MOF) of [Co(OH)(TCA)(HO)] (abbreviation: JXNU-9) based on the unique octanuclear Co(μ-OH) clusters linked by 4,4',4″-nitrilotribenzoate (TCA) ligands featuring small caged structures and one-dimensional channels was prepared and characterized. JXNU-9 shows a high CH uptake capacity of 3.60 mmol g (4.

Effectiveness and safety of electroacupuncture in treating Parkinson disease: A protocol for systematic review and meta-analyses.

Background: Parkinson disease (PD) is an Extrapyramidal Disease mainly characterized by static tremor, myotonia, bradykinesia and postural gait disorder. As China's population ages, the number of people suffering from PD is increasing. Since there are many side effects of western medicine for Parkinson's patients, and the high price of the drugs make it difficult for many patients to adhere to treat.

[Effects of no-tillage, mulching, and organic fertilization on soil fungal community composition and diversity].

We examined the responses of soil fungal community to no-tillage, mulching, and organic fertilization by setting up different treatments for three years in winter wheat land in mountain areas of southern Ningxia, including no-tillage, mulching and organic fertilization (NF), no-tillage, mulching and no organic fertilization (NC), conventional tillage without mulching and organic fertilization (TF), and conventional tillage without mulching and no organic fertilization (TC). Based on Illumina Miseq high-throughput sequencing platform, the relationship between the composition and diversity of soil fungal community and soil environmental factors were examined. A total of 3490 fungal OTUs were obtained from four treatments, which included some unidentified or unknown fungi.

Highly active bidentate N-heterocyclic carbene/ruthenium complexes performing dehydrogenative coupling of alcohols and hydroxides in open air.

Eight bidentate NHC/Ru complexes, namely [Ru]-1-[Ru]-8, were designed and prepared. In particular, [Ru]-2 displayed extraordinary performance even in open air for the dehydrogenative coupling of alcohols and hydroxides. Notably, an unprecedentedly low catalyst loading of 250 ppm and the highest TON of 32 800 and TOF of 3200 until now were obtained.

Assessing the performance of monocyte to high-density lipoprotein ratio for predicting ischemic stroke: insights from a population-based Chinese cohort.

Background: Monocyte to high-density lipoprotein cholesterol ratio (MHR) is a recently emerged measure of inflammation and oxidative stress and has been used to predict multiple cardiovascular abnormalities, but data relative to ischemic stroke are lacking. The goal of this study was to estimate the associations of MHR and prevalent ischemic stroke among a large cohort of general Chinese population.

Method: The study analyzed 8148 individuals (mean age: 54.

Clinical Features and Correlates of Excessive Daytime Sleepiness in Parkinson's Disease.

To explore the clinical features and correlates of excessive daytime sleepiness (EDS) in a Chinese population of Parkinson's disease (PD) patients. Patients with clinically established or clinically probable PD were recruited. Clinical and demographic data were collected, and participants were evaluated using standardized assessment protocols.

CRISPLD2 is expressed at low levels during septic shock and is associated with procalcitonin.

Introduction: Previous studies have shown that cysteine-rich secretory protein containing LCCL domain 2 (CRISPLD2) is a novel lipopolysaccharide (LPS)-binding protein, and the upregulation of CRISPLD2 expression protects mice against LPS-induced lethality. The aim of this study was to examine the expression of CRISPLD2 in patients with sepsis and characterize the association of this protein with procalcitonin.

Methods: The expression of CRISPLD2 was determined in 100 healthy volunteers and 119 septic patients.

Insulin receptor tyrosine kinase substrate enhances low levels of MDM2-mediated p53 ubiquitination.

The tumor suppressor p53 controls multiple cellular functions including DNA repair, cell cycle arrest and apoptosis. MDM2-mediated p53 ubiquitination affects both degradation and cytoplasmic localization of p53. Several cofactors are known to modulate MDM2-mediated p53 ubiquitination and proteasomal degradation.

The novel lipopolysaccharide-binding protein CRISPLD2 is a critical serum protein to regulate endotoxin function.

LPS is an immunostimulatory component of Gram-negative bacteria. Acting on the immune system in a systemic fashion, LPS exposes the body to the hazard of septic shock. In this study we report that cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2/Crispld2; human and mouse/rat versions, respectively), expressed by multitissues and leukocytes, is a novel LPS-binding protein.

Excretory/secretory proteome of the adult developmental stage of human blood fluke, Schistosoma japonicum.

Schistosomes are the causative agents of schistosomiasis, one of the most prevalent and serious of the parasitic diseases that currently infects approximately 200 million people worldwide. Schistosome excretory/secretory (ES) proteins have been shown to play important roles in modulating mammalian host immune systems. In our current study, we performed a global proteomics identification of the ES proteins from adult worms of Schistosoma japonicum, one of the three major schistosome species.

Liver-specific ZP domain-containing protein (LZP) as a new partner of Tamm-Horsfall protein harbors on renal tubules.

Liver-specific ZP domain-containing protein (LZP) was recently identified as a secreted protein that is specifically expressed in liver. However, the physiological effects of LZP are largely unknown. In this study, we found that LZP was detectable in mouse kidneys, testes, ovaries and heart, in addition to liver.

SjTPdb: integrated transcriptome and proteome database and analysis platform for Schistosoma japonicum.

Background: Schistosoma japonicum is one of the three major blood fluke species, the etiological agents of schistosomiasis which remains a serious public health problem with an estimated 200 million people infected in 76 countries. In recent years, enormous amounts of both transcriptomic and proteomic data of schistosomes have become available, providing information on gene expression profiles for developmental stages and tissues of S. japonicum.

hZG16, a novel human secreted protein expressed in liver, was down-regulated in hepatocellular carcinoma.

Here we reported a novel human secreted protein named as hZG16, with a Jacalin domain. Evolution analysis through comparing with the orthologs of other organisms suggested that ZG16 is a conserved gene under the purifying selection (d(N)/d(s)<1) in the evolution. Interestingly, Northern and dot blot analyses showed that hZG16 were highly expressed in adult liver, not in fetal liver, and moderately in gut, including jejunum, ileum, and colon, in which the tissue expression pattern of hZG16 was significantly dissimilar to that of mouse and rat orthologs that were uniquely expressed in spleen and pancreas, respectively.

Insight into the host-parasite interplay by proteomic study of host proteins copurified with the human parasite, Schistosoma japonicum.

The tegument proteins of schistosome have attracted the most attention in studies of host-parasite interplay, while the host proteins acting at the host-parasite interface remained largely elusive. Here, we undertook a high-throughput proteomic approach to characterize the schistosome-adsorbed host proteins. Fifty five distinct host proteins were confidently identified in S.

Up-regulation of DLK1 as an imprinted gene could contribute to human hepatocellular carcinoma.

Dysregulation of a genomic imprinting gene can contribute to carcinogenesis. Here, delta-like 1 homolog (Drosophila) (DLK1), a paternally expressed gene, was found to be significantly up-regulated in 60 (73.2%) of a total of 82 hepatocellular carcinoma (HCC) specimens using reverse transcription-polymerase chain reaction.

New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum.

Schistosomiasis remains a serious public health problem with an estimated 200 million people infected in 76 countries. Here we isolated ~ 8,400 potential protein-encoding cDNA contigs from Schistosoma japonicum after sequencing circa 84,000 expressed sequence tags. In tandem, we undertook a high-throughput proteomics approach to characterize the protein expression profiles of a number of developmental stages (cercariae, hepatic schistosomula, female and male adults, eggs, and miracidia) and tissues at the host-parasite interface (eggshell and tegument) by interrogating the protein database deduced from the contigs.