[Analysis of influencing factors of early femoral head necrosis after femoral neck fracture and construction of nomogram prediction model].

Objective: To explore the risk factors of early femoral head necrosis in patients with femoral neck fracture after operation, and to establish a nomogram prediction model.

Methods: A total of 167 patients with femoral neck fracture from January 2020 to April 2022 were selected and divided into necrosis group and non-necrosis group according to whether femoral head necrosis occurred in the early postoperative period. There were 21 males and 17 females in the necrosis group, aged from 33 to 72 years old, with an average of (53.

High prevalence of Schistosoma japonicum by perfusion in naturally exposed water buffalo in a region of the Philippines endemic for human schistosomiasis.

In the past decade, ecological surveys emphasized rats and dogs as the most significant animal reservoirs for Schistosoma japonicum (S.j) in the Philippines. However, recent studies demonstrated 51-91% prevalence of schistosomiasis among water buffalo using qPCR in the Sj endemic regions in the Philippines.

[Effects of increased planting density with reduced nitrogen fertilizer application on greenhouse gas emission in double-season rice fields under water saving and simple cultivation mode].

To clarify the effects of increased density and reduced nitrogen on greenhouse gas emission in double-season paddy fields under the water-saving and simple cultivation mode, we used Luliangyou 996 (early rice) and Fengyuanyou 299 (late rice) as materials to collect greenhouse gas by closed static box method, monitored the dynamics of CH and NO emissions from different combinations of increased density and reduced nitrogen for early and late rice, and explored changes in cumulative CH and NO emissions, global warming potential (GWP) and greenhouse gas intensity (GHGI) as affected by different combinations of increased density and reduced nitrogen from double-season rice fields. The results showed that the cumulative emissions of CH and NO from different combinations were significantly different. Compared with the control (CK), cumulative CH emission, GWP and GHGI in the two seasons decreased by 50.

[Effects of winter cropping mode on soil organic carbon and carbon management index of double rice paddy].

To explore the effects of winter crops recycling into paddy field on soil carbon pool, we cultivated rape, Chinese milk vetch, ryegrass and potato in winter season after harvesting double-season rice, with fallow as control (CK). The contents of soil organic carbon (SOC) and labile organic carbon (LOC) in the harvest of early and late rice were measured, and the stable carbon, activity of carbon pool, carbon pool index, carbon pool activity index and carbon pool management index (CMI) were calculated. Results showed that winter cover crops returning increased SOC content by 1%-8% and 3%-18% after harvest of early rice and late rice, respectively in comparison with CK.

[Differences and Relationship Between Rhizosphere Characteristics and Methane Emissions of Double-cropping Rice Variety].

Six field varieties of early rice and late rice were selected as test materials for field experiments to explore the difference in CH emissions among different rice varieties, and Static Obscura-Gas Chromatography was used to determine the CH gas. The results demonstrated a significant difference in the CH emissions flux between early and late rice varieties. The average yield of total fertility CH emissions was highest in Xiangzaoxian 24 and lowest in Zhuliangyou 819, with a difference of 34.

Microdissected thin anterolateral thigh perforator flaps with multiple perforators: A series of case reports.

Introduction: The study aimed to explore the effect of microdissected thin anterolateral thigh (MTALT) perforator flap with multiple perforators on patients with complex defects on the hand, elbow, heel, or knee.

Methods: From March 2012 to February 2013, 5 patients with complex defects on the hand, elbow, heel, or knee were included. During the flap preparation, 2 to 3 perforators penetrating the fascia of the anterolateral femoral area were initially detected, and the deep fascia was incised.

Vaccination with recombinant paramyosin in Montanide ISA206 protects against Schistosoma japonicum infection in water buffalo.

Background: Schistosomiasis japonica is a zoonosis and presents significant public health problems in China and the Philippines. Vaccines targeting domestic animals constitute attractive control measures.

Methods: We conducted three vaccine trials to evaluate the protective efficacy of recombinant full-length paramyosin (rSj97) in water buffalo.

Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals.

Background: Schistosomiasis japonica is a common zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and, for this reason, diagnostics with a higher sensitivity have become increasingly necessary.

Bipolar diathermy-assisted coblation really affects post-tonsillectomy haemorrhage rate and white membrane in paediatric tonsillectomy.

Unlabelled: Bipolar diathermy-assisted coblation really affects post-tonsillectomy haemorrhage rate and white membrane in paediatric tonsillectomy.

Objective: this study aimed to evaluate the effect of bipolar diathermy-assisted coblation on post-tonsillectomy haemorrhage (PTH) rate, operation time, the volume of blood loss during surgery, the time needed to return to a regular diet and the relationship between the thickness of the white membrane of tonsil fossa and the degree of postoperative pain in paediatric tonsillectomy.

Methods: a total of 1684 patients subjected to tonsillectomy (TE) were enrolled in this study from June 2013 to December 2015.

[Effects of Water and Nitrogenous Fertilizer Coupling on CH4 and N2O Emission from Double-Season Rice Paddy Field].

To provide support for the efficient use of water and fertilizer technology to double-season rice cultivation, water and fertilizer coupling mode was applied in this research, including two irrigation methods and four N levels. The irrigation methods were flood irrigation and intermittent irrigation, while four N levels were high-N, middle-N, low-N and none-N. Field experiment was conducted to study the effect of water and fertilizer coupling mode on CH4 and N2O emission.

Linc00675 is a novel marker of short survival and recurrence in patients with pancreatic ductal adenocarcinoma.

Aim: To detect linc00675 expression in pancreatic ductal adenocarcinoma (PDAC), to analyze the relationship between the expression level of linc00675 and the clinical pathological characteristics, to explore the biological functions of linc00675, and to determine whether linc00675 has independent prognostic value in PDAC.

Methods: We studied linc00675 expression among eight histologically confirmed PDAC tissue samples and four chronic pancreatitis tissue samples through microarray screening. RT-qPCR was conducted to further investigate linc00675 expression in PDAC cell lines as well as archived tissues from a large cohort of PDAC patients.

Low RASSF6 expression in pancreatic ductal adenocarcinoma is associated with poor survival.

Aim: To analyze RASSF6 expression in pancreatic ductal adenocarcinoma (PDAC) and to determine whether RASSF6 has an independent prognostic value in PDAC.

Methods: We studied RASSF6 expression in 96 histologically confirmed PDAC samples and 20 chronic pancreatitis specimens using immunohistochemistry and real-time quantitative reverse transcription-PCR. PDAC issues were then classified as RASSF6 strongly positive, weakly positive or negative.

Chemokine ligand 20 enhances progression of hepatocellular carcinoma via epithelial-mesenchymal transition.

Aim: To identify the mechanisms of chemokine ligand 20 (CCL20)-induced hepatocellular carcinoma (HCC) metastasis and evaluate it as a prognostic marker.

Methods: Expression of CCL20 was evaluated by immunohistochemistry in HCC tissues from 62 patients who underwent curative resection. The relationship between CCL20 expression and clinicopathologic features was analyzed.

[Longitudinal observation of epidemic dynamics of schistosomiasis in bovine in two mountainous endemic regions].

Objective: To understand the endemic situation dynamics of schistosomiasis in domestic animals (mainly bovine) in mountainous endemic regions, so as to provide the reference for evaluating the control effect and improving control strategy.

Methods: Two representative pilots (Renmei and Dacang) in mountainous schistosomiasis endemic regions were selected for survey. The schistosome infection status of bovine was investigated by the miracidium hatching method, the pasture of bovine were investigated by home visiting, and the distributions of wild feces and Oncomelania snails, and the snail schistosome infection status were also investigated in April and September every year.

[Identification and preliminary analysis of a novel full-length cDNA encoding retinoid X receptor 2 from Schistosoma japonicum].

Objective: To clone and preliminarily analyze the full-length cDNA encoding retinoid X receptor 2 (RXR2) from Schistosoma japonicum.

Methods: The rapid amplification cDNA ends (RACE)was applied to get a full-length cDNA encoding retinoid X receptor 2 from S. japonicum (SjRXR2).

[Cloning, expression and immune function analysis of a gene encoding inositol monophosphate in Schistosoma japonicum].

Objective: To clone and express a full-length cDNA encoding inositol monophosphate of Schistosoma japonicum (SjIM), and to access its immunoprotection in BALB/c mice for schistosomisis.

Methods: A full-length cDNA encoding the S. japonicum inositol monophosphate was isolated from 42 d schistosomes cDNAs.

[Screening and verification on characteristic differentially expressed genes of Schistosoma japonicum from three reservoir hosts].

Objective: To get the characteristic differentially expressed genes of Schistosoma japonicum from three important reservoir hosts: yellow cattle, water buffalo and goat, so as to find the genetic markers to identify the various sources of the parasite reservoir hosts.

Methods: The 49 d worms were collected from artificially infected animals, and the total RNA(s) of worms were extracted and reverse-transcripted to cDNA, and then hybridized with custom-built microarray to screen characteristic differentially expressed genes of every host, and the microarray results were validated by the real-time PCR method.

Results: From results of microarray, we got 3 characteristic differentially expressed genes of S.

[Characterization of CH4, N2O emission and selection of rice cultivars in double cropping rice fields].

Methane and nitrous oxide emission fluxes of rice cultivars in the double cropping rice fields were measured by static chamber-gas chromatography in field experiments. The results showed a single-peak pattern in seasonal variations of methane emission and a double-peak pattern in variations of nitrous oxide emission for the early season rice, and a single-peak pattern in variations of both methane and nitrous oxide emission for the late season rice. There was significant difference among the rice cultivars in the average emission fluxes of methane and nitrous oxide during the whole growing season.

[Study of intratympanic methylprednisolone injections in diabetics with a sudden sensorineural hearing loss].

Objective: To evaluate the effects of intratympanic methylprednisolone injection by microcatheter in diabetics with a sudden hearing loss.

Methods: From July 2005 to November 2009, 113 diabetics with a sudden hearing loss within an onset of 10 days at our department were recruited. But they received no previous intervention and were assigned to treatment and control groups.

[Evaluation on the immuno-protective efficacy of the recombinant antigen SjPGAM-SjEnol against Schistosoma japonicum in mice].

Objective: To construct and express the recombinant plasmid pET32a-SjPGAM-SjEnol and evaluate its immuno-protective efficacy against the infection of Schistosoma japonicum in mice.

Methods: The peptides of SjPGAM and SjEnol containing the multivalent epitopes with higher binding capacity of human MHC II and mouse H2-dII but low homology with the host were analyzed and screened through bioinformatics. The corresponding nucleotide sequence of selected epitopes was spliced and the recombinant plasmid pET32a-SjPGAM-SjEnol was constructed and expressed in Escherichia coli BL21 cells.

[Effects of high-yielding rice cultivar and cultivation pattern on methane emission from paddy field].

A field experiment was conducted to observe the effects of high-yielding rice cultivars (early rice Luliangyou No. 996 and Chuanfeng No. 1, and late rice T you No.

[Immune protection of tegument protein rSj29 against Schistosoma japonicum in mice].

Objective: To clone, express and characterize a tegument protein gene of Schistosoma japonicum (Sj29) , and investigate the immune protection of the recombinant protein against S. japonicum in mice.

Methods: The gene coding for Sj29 protein was amplified by PCR, and the sequence was analyzed by bioinformatics tools.

Comparison of Recombinant Proteins from Schistosoma japonicum for Schistosomiasis Diagnosis.

The most important animal reservoirs of Schistosoma japonicum in China are bovines. Diagnosis and control of bovine schistosomiasis is critical for reducing the prevalence of the disease. We screened defined diagnostic antigens that have the potential to increase the sensitivity and specificity of serological assays and to distinguish between active and prior infections.

[Construction of phage display cDNA library from adult worms of Schistosoma japonicum].

Objective: To screen protective antigen genes and construct the T7 phage display library from adult worms of Schistosoma japonicum.

Methods: Total RNA was extracted from adult worms of S. japonicum by Trizol reagent anti mRNA was isolated from the total RNA.