Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells the PI3K/AKT pathway.

Background: Heterogeneous ribonucleoprotein A1 (hnRNPA1) has been reported to enhance the Warburg effect and promote colon cancer (CC) cell proliferation, but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC have not yet been elucidated.

Aim: To investigate the role and mechanism of a novel miR-490-3p/hnRNPA1-b/PKM2 axis in enhancing the Warburg effect and promoting CC cell proliferation through the PI3K/AKT pathway.

Methods: Paraffin-embedded pathological sections from 220 CC patients were collected and subjected to immunohistochemical analysis to determine the expression of hnRNPA1-b.

A water stable Eu(III)-organic framework as a recyclable multi-responsive luminescent sensor for efficient detection of -aminophenol in simulated urine, and Mn and Cr anions in aqueous solutions.

A novel 3D Eu(iii) metal-organic framework (Eu-MOF-1) formulated as [Eu(L)(H2O)(DMA)] (L = 2-(2-nitro-4-carboxylphenyl)terephthalic acid) has been successfully synthesized under solvothermal conditions and characterized by structural analyses. Eu-MOF-1 displays a new 3D framework containing EuIII ions, ligand L, and coordinated DMA molecules and water molecules. The fluorescence investigations indicate that Eu-MOF-1 emits bright red luminescence, and shows relatively high water stability and outstanding chemical stability under a relatively wide range of pH conditions.

Water-stable Cd(ii)/Zn(ii) coordination polymers as recyclable luminescent sensors for detecting hippuric acid in simulated urine for indexing toluene exposure with high selectivity, sensitivity and fast response.

Three novel Cd(ii)/Zn(ii) coordination polymers (CPs), namely [Cd(L)(BPDC)HO]·0.5HO (1), [Zn(L)(BPDC)]·2HO (2) and [Cd(L)(BTC)HO]·3HO (3) (L = 4-(tetrazol-5-yl)phenyl-4,2':6',4''-terpyridine, HBPDC = 4,4'-biphenyldicarboxylic acid, and HBTC = 1,3,5-benzenetricarboxylic acid), have been successfully synthesized and characterized. CP 1 and CP 2 display new two-dimensional double-layered honeycomb frameworks containing uncoordinated nitrogen atoms from pyridine and tetrazole rings, which can easily form hydrogen bonds with various analytes.

3D Ln-MOFs: slow magnetic relaxation and highly sensitive luminescence detection of Fe and ketones.

Five new 3D isostructural lanthanide metal organic frameworks (Ln-MOFs), [Ln(HL)1.5(H2O)(DMF)]·2H2O (Ln = GdIII (1), SmIII (2), DyIII (3), EuIII (4) and TbIII (5), H3L = 5-(3',5'-dicarboxylphenyl) nicotinic acid) were synthesized by solvothermal methods and studied by structural analyses, magnetic analyses and luminescence sensing. Crystallographic studies revealed that these compounds are 3D frameworks in which the LnIII-COO chains with alternating four and two carboxylate bridges are interlinked by the organic ligands L, and contain microporous channels with accessible Lewis-base sites, coordinated water molecules and uncoordinated carboxyl groups, which are easy to combine and recognise various analytes.

[Expression of key enzyme genes and content of saikosaponin in saikosaponin biosynthesis under drought stress in Bupleurum chinense].

To research the expression of key enzymes in saikosaponin biosynthesis and the content of saikosaponin under the drought stress, the study focused on the gene-level and the end product responses to environmental change. Taking the five months of Bupleurum chinense as research materials, the contents of saikosaponin A and saikosaponin D under different stress levels were measured by HPLC. The drought was simulated by poly ethylene glycol.

Chinese Internet Searches Provide Inaccurate and Misleading Information to Epilepsy Patients.

Background: Most patients with epilepsy want to learn as much as possible about the disease, and many have turned to the internet for information. Patients are likely to use information obtained from the internet to control their epilepsy, but little is known about the accuracy of this information. In this survey, we have assessed the feasibility and usability of internet-based interventions for the treatment of epilepsy.

Blockade of presynaptic 4-aminopyridine-sensitive potassium channels increases initial neurotransmitter release probability, reinstates synaptic transmission altered by GABAB receptor activation in rat midbrain periaqueductal gray.

The activation of γ-aminobutyric acid receptor subtype B (GABAB) receptors in the midbrain ventrolateral periaqueductal gray (vlPAG) induces both postsynaptic and presynaptic inhibition. Whereas the postsynaptic inhibition is mediated by G protein-coupled inwardly rectifying K channels, the presynaptic inhibition of neurotransmitter release is primarily mediated by voltage-gated calcium channels. Using whole-cell recordings from acute rat PAG slices, we report here that the bath application of 4-aminopyridine, a voltage-gated K channel blocker, increases the initial GABA and glutamate release probability (P) and reinstates P depressed by presynaptic GABAB receptor activation at inhibitory and excitatory synapses, respectively.

Clinicopathological study of sporadic Burkitt lymphoma in children.

Background: Non-Hodgkin lymphoma is the fourth most common malignant tumors in children, Burkitt lymphoma (BL) accounts for 30-50% of all pediatric lymphomas. The aim of this study was to investigate the clinicopathologic features, immunophenotype, Epstein-Barr virus (EBV) infection and c-myc gene rearrangement of sporadic BL in children.

Methods: Ninety-two cases of pediatric BL were retrospectively analyzed for clinical features, immunohistochemistry, EBV-encoded RNA (EBER) status by in situ hybridization and c-myc gene rearrangement by fluorescence in situ hybridization.

[Effect of Eu ions on the Ag nanoparticles precipitation and their optical properties in borate glasses].

Eu-Ag co-doped borate glasses were prepared by the high temperature solid method in the present work. Absorption and emission spectra were employed to investigate the precipitation of Ag nanoparticles, which is influenced by the network form B2O3 and the co-doped Eu ions. It was found in the absorption spectra of Eu-Ag co-doped sample that a broad band centered at about 410 nm emerged and their intensity decreased with the increase in the BZ 03 concentration.

GABAB receptors resist acute desensitization in both postsynaptic and presynaptic compartments of periaqueductal gray neurons.

The ventrolateral midbrain periaqueductal gray (PAG) neurons have been intensively studied because of their pivotal role in the descending pain modulation system. Activation of GABAB receptors, one type of inhibitory G-protein-coupled receptors (GPCRs), in PAG neurons results in both presynaptic and postsynaptic inhibition. Acute desensitization is defined as rapid attenuation of receptor-mediated signaling.

Bis[4-amino-3,5-bis-(pyridin-2-yl)-4H-1,2,4-triazole-κ(2)N(1),N(5)]diaqua-cobalt(II) bis-(perchlorate).

In the title structure, [Co(C(12)H(10)N(6))(2)(H(2)O)(2)](ClO(4))(2), the Co(II) atom lies on an inversion centre and is coordinated in a slightly distorted octa-hedral geometry by four N atoms from two 4-amino-3,5-bis-(pyridin-2-yl)-4H-1,2,4-triazole (adpt) ligands in equatorial positions and two O atoms from two water mol-ecules in axial positions. An intra-molecular N-H⋯N inter-action stabilizes the mol-ecular conformation. Inter-molecular N-H⋯O and O-H⋯O inter-actions involving the perchlorate counter-anions extend the monomeric compound into a two-dimensional network parallel to the bc plane.

Neural differentiation ability of mesenchymal stromal cells from bone marrow and adipose tissue: a comparative study.

Background Aims: The characteristics, such as morphologic and phenotypic characteristics and neural transdifferentiation ability, of mesenchymal stromal cells (MSC) derived from different origins have yet to be reported for cases isolated from the same individual.

Methods: The proliferation capacity, secretion ability of neurotrophins (NT) and neural differentiation ability in rat MSC isolated from bone marrow (BMSC) and adipose tissue (ADSC) were compared from the same animal.

Results: The ADSC had a significantly higher proliferation capacity than BMSC according to cell cycle and cumulative population doubling analyses.

Bis(2-amino-5-methyl-1,3,4-thia-diazole-κN(3))dichloridocobalt(II).

In the monomeric title complex, [CoCl(2)(C(3)H(5)N(3)S)(2)], the Co(II) atom is tetra-coordinated by two chloride anions and two N atoms from two monodentate 2-amino-5-methyl-1,3,4-thia-diazole ligands, giving a slightly distorted tetra-hedral stereochemistry [bond angle range about Co = 105.16 (12)-112.50 (10)°].

Potassium channels underlie postsynaptic but not presynaptic GABAB receptor-mediated inhibition on ventrolateral periaqueductal gray neurons.

γ-Aminobutyric acid (GABA) is the principle inhibitory neurotransmitter in adult mammalian brain. GABA receptors B subtype (GABA(B)Rs) are abundantly expressed at presynaptic and postsynaptic neuronal structures in the rat ventrolateral periaqueductal gray (PAG), an area related to pain regulation. Activation of GABA(B)Rs by baclofen, a selective agonist, induces presynaptic inhibition by decreasing presynaptic glutamate release.

Bis[3-(pyrazin-2-yl)-5-(pyridin-2-yl-κN)-1,2,4-triazol-1-ido-κN(1)]copper(II).

In the mononuclear title complex, [Cu(C(11)H(7)N(6))(2)], the Cu(II) atom lies on a crystallographic inversion centre and is coordinated by four N atoms from two bidentate chelate monoanionic 3-(pyrazin-2-yl)-5-(pyridin-2-yl-1,2,4-triazol-1-ido ligands, two from the triazolide rings [Cu-N = 1.969 (2) Å] and two from the pyridine rings [Cu-N = 2.027 (2) Å], giving a slightly distorted square-planar geometry.

[Biocompatibility of a novel biological piezoelectric ceramic to the rat periosteum derived osteoblast].

Objective: A novel biological piezoelectric ceramic was made by beta-tricalcium phosphate (beta-TCP) and lithium sodium potassium niobate (LNK) piezoelectric ceramics. To study its biocompatibility to osteoblast isolated from the cranium of 1-day-old Sprague-Dawley mice.

Methods: The biological piezoelectric ceramic TCPLNK1/10, TCPLNK5/5 respectively mixed by beta-TCP and LNK piezoelectric ceramic at the ratio of 1/10 and 5/5.

Tris(2,2'-bioxazoline-kappa2N,N')copper(II) diperchlorate and tris(2,2'-bioxazoline-kappa2N,N')nickel(II) diperchlorate.

In the two isomorphous title compounds, viz. tris[2,2'-bi(4,5-dihydro-1,3-oxazole)-kappa(2)N,N']copper(II) diperchlorate, [Cu(C6H8N2O2)3](ClO4)2, (I), and tris[2,2'-bi(4,5-dihydro-1,3-oxazole)-kappa2N,N']nickel(II) diperchlorate, [Ni(C6H8N2O2)3](ClO4)2, (II), the MII ions each have a distorted octahedral coordination geometry formed via six N atoms from three 2,2'-bioxazoline ligands. For each ligand, the two five-membered rings are nearly coplanar.

[Responses of neurons and astrocytes in rat hippocampus to kainic acid-induced seizures].

Objective: To investigate the time course of the responses of neurons and astrocytes in rat hippocampus (HI) to kainic acid (KA)-induced seizures in various regions.

Methods: By means immunohistochemical staining for anti-Fos protein and anti-glial fibrillary acidic protein (GFAP), the regional distribution of reactive neurons and astrocytes in the HI was observed at different time points after a unilateral stereotaxic microinjection of KA into the lateral ventricle of rats to cause limbic and generalized convulsive seizures.

Results: The injection of KA triggered limbic motor seizures including immobilization, staring, facial and jaw clonus ect.

Dipole localization in surgical treatment of epileptic foci secondary to intracranial mass lesions: retrospective analysis of 47 cases.

Objective: To assess the value of dipole localization in the surgical treatment of epileptic foci arising from intracranial mass lesions and observe the long-term effect of this technique.

Method: With the assistance if dipole localization system for epileptic foci and magnetic resonance imaging (MRI), we performed precise localization of the epileptic foci secondary to the intracranial mass lesions both preoperatively and during the surgeries in 47 cases. The clinical features of this type of epilepsy and the precautions essential for the operations were reviewed, with the long-term outcome of the patients analyzed on the basis of a follow-up study for at least 1 year of all these cases.

Temporal lobe epilepsy surgery and preoperative factors predictive of postoperative outcome: retrospective analysis of 143 cases.

Objective: To evaluate the long-term outcome of temporal lobe epilepsy (TLE) surgery with dipole localization and to analyze the preoperative factors predicting a satisfactory postoperative outcome of the patients.

Methods: A total of 143 patients, who underwent TLE surgery with dipole localization combined with magnetic resonance imaging between 1999 and 2001 and were followed up for at least 1 year, were enrolled in this retrospective analysis of clinical, electrophysiological, neuroimaging and surgical factors to determine the independent predictors for the clinical outcome of the patients.

Results: During the follow-up with a mean duration of 27.

Ultrastructrural observation of the membrane surface of kainic acid-treated hippocampal neurons by atomic force microscopy.

Objective: To observe the three-dimensional morphological changes on the membrane surface of primary cultured rat hippocampal neurons in response to kainic acid (KA) exposure.

Methods: After isolation and primary culture, Wistar rat hippocampal neurons were treated with KA at the concentrations of 0, 25, and 250 micromol/L for different durations (10 and 100 min) to observe the subsequent changes in the membrane surface structure of the neurons by nano-scale scanning with an atomic force microscope (AFM).

Results: Normal neurons displayed smooth membrane surface with even and regular undulation, while the neurons treated with KA, in contrast, presented degenerative changes characterized by cell swelling and coarse membrane surface with processes and holes.

Effect of kainate on the synaptic transmission in hippocampal CA1 region.

Objective: To investigate the effect of activated kainate receptor on both the excitatory and inhibitory synaptic transmission in the neurons in the hippocampal CA1 region.

Method: Blind whole-cell voltage-clamp recordings were performed on the CA1 pyramidal cells in adult rat hippocampal slices to examine and analyze the effect of bath-applied kainate (10 micromol/L) on CA1 afferent fiber-evoked excitatory postsynaptic currents (EPSCs) and inhibitory postsynaptic currents (IPSCs), respectively.

Results: Activation of kainate receptor significantly depressed both IPSCs (P <0.

Surgical treatment for intractable epilepsy guided by dipole localization.

Objective: To evaluate the effect of surgical treatment of intractable epilepsy under the guidance of dipole localization (DL).

Method: From 1999 to 2001, 240 patients with intractable epilepsy received examination with electroencephalography and their epileptic foci identified by DL before the implementation of operations ranging from epileptogenic focus resection to lobectomy, anterior corpus callosotomy or multiple subpial transection. Follow-up study was conducted in 180 of the patients for over 6 months.