The pivotal role of CRIHSP sequences in orchestrating antigen receptor diversity and genomic stability within antigen receptor germline genes.

The mechanisms underlying antigen receptor germline gene diversification have always been a topic of intensive study. Here, we discovered that the frequency of stem-loop sequences in the antigen receptor germline gene region is remarkably higher than the genomic background. By analyzing these stem-loop sequences' similarity and distribution patterns, we found that clustered regularly interspaced homologous stem-loop pairs (CRIHSP) are widely present on the germline genes of antigen receptors in different species.

Electroacupuncture combined with trigonelline inhibits pyroptosis in cerebral ischemia-reperfusion by suppressing autophagy via the PI3K/AKT/mTOR signaling pathway.

Background: Electroacupuncture (EA) and trigonelline (TG) have been reported to be beneficial in alleviating cerebral ischemia/reperfusion injury (CIRI). However, the synergistic effects of EA and TG in CIRI and the underlying mechanism have not been demonstrated.

Methods: Rats were subjected to middle cerebral artery occlusion (MCAO) surgery and reperfusion (MCAO/R) to establish a CIRI model.

Tumor microenvironment and immunotherapy for triple-negative breast cancer.

Triple-negative breast cancer (TNBC) is a subtype of breast cancer known for its high aggressiveness and poor prognosis. Conventional treatment of TNBC is challenging due to its heterogeneity and lack of clear targets. Recent advancements in immunotherapy have shown promise in treating TNBC, with immune checkpoint therapy playing a significant role in comprehensive treatment plans.

METTL4 and METTL5 as biomarkers for recurrence-free survival in hepatocellular carcinoma patients.

Background: Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths, with high rates of postoperative recurrence. Identifying reliable biomarkers for predicting recurrence is critical for improving patient outcomes. This study investigates the predictive value of m6A methylation-related genes, METTL4 and METTL5, on HCC recurrence after surgery.

Polystyrene nanoplastics sequester the toxicity mitigating potential of probiotics by altering gut microbiota in grass carp (Ctenopharyngodon idella).

This study evaluated the role of probiotics in enhancing intestinal immunity and mitigating polystyrene nanoplastics (PS-NPs)-induced toxicity in grass carp (Ctenopharyngodon idella). Grass carp were fed probiotics (Bacillus subtilis, Bacillus velezensis, Lactobacillus reuteri, and Lactococcus lactis) for two weeks before being exposed to PS-NPs for five days. Probiotic pretreatment alleviated PS-NPs-induced intestinal damage, with Bacillus velezensis and Lactococcus lactis groups showing milder vacuolation and villus breakage than other groups.

Degradation of 1,2,3-trichloropropane by ferrate(VI) oxidant: Mechanisms, influencing factors and oxidative iron species.

The ferrate(VI) ion is a green, versatile oxidant employed eliminate organic contaminants. Research on the degradation of saturated chlorinated hydrocarbons such as 1,2,3-trichloropropane (1,2,3-TCP), by Fe(VI) is limited. In this study, we investigated the degradation of 1,2,3-TCP by Fe(VI).

Longitudinal changes in plasma Cystatin C and all-cause mortality risk among the middle-aged and elderly Chinese population.

Objective: Elevated plasma Cystatin C levels are associated with an increased mortality risk among middle-aged and elderly Chinese individuals. This study explores whether tracking the longitudinal changes in Cystatin C can improve the prediction of mortality risk and allow better risk stratification, jointly with baseline measurements.

Design & Methods: This analysis includes 3,195 participants from the China Health and Retirement Longitudinal Study who completed plasma Cystatin C measurements in two waves (2011 and 2015) and were followed through 2020.

Hydrogen Radical Enabling Industrial-Level Oxygen Electroreduction to Hydrogen Peroxide.

The electrochemical synthesis of hydrogen peroxide from oxygen and water, powered by renewable electricity, provides a highly attractive alternative to the energy-intensive autoxidation process presently used in industry, but much remains unknown about this two-electron oxygen reduction reaction (2e-ORR), especially the local proton effect. Here, we have investigated the function of hydrogen-associated intermediates in the 2e-ORR using a rationally designed cooperative electrode material with cobalt (II) clusters embedded onto the oxidized carbon nanotube composites (Co-OCNT). We found that the local proton availability can determine both the reaction kinetics and selectivity.

SDC4 protein action and related key genes in nonhealing diabetic foot ulcers based on bioinformatics analysis and machine learning.

Diabetic foot ulcers (DFU) is a complication associated with diabetes characterised by high morbidity, disability, and mortality, involving chronic inflammation and infiltration of multiple immune cells. We aimed to identify the critical genes in nonhealing DFU using single-cell RNA sequencing, transcriptomic analysis and machine learning. The GSE165816, GSE134431, and GSE143735 datasets were downloaded from the GEO database.

Development of an optimized SEC method for characterization of genome DNA leakage from adeno-associated virus products.

Adeno-associated virus (AAV) vectors are widely used to deliver therapeutic transgenes due to their superior safety, relatively low immunogenicity, and ability to target diverse tissues. AAV gene therapy products are typically formulated as frozen liquid and stored below - 60 °C, and therefore are subjected to multiple freeze/thaw cycles during manufacturing and administration. Recent studies have shown that genome DNA leakage could be induced by freeze/thaw stress.

Sulfonated Covalent Organic Frameworks Anchoring Cobalt as High-Efficient and Stable Catalysts for Peroxymonosulfate Activation.

Heterogeneous cobalt-based catalysts are highly effective in activing peroxymonosulfate (PMS) and produce free radicals to deal with recalcitrant organic pollutants in water. However, unfeasible recyclability and gradual performance degradation remain challenging due to the easy agglomeration and leaching of active cobalt species. Herein, a strategy is proposed to construct stably anchored and highly dispersed Co sites on dual functional sulfonated covalent organic frameworks (COF-Co).

Case Report: PROS1 (c.76+2_76+3del) pathogenic mutation causes pulmonary embolism.

Background: Genetic variation plays an extremely important pathogenic role in the development of venous thromboembolism (VTE). Genetic protein S (PS) deficiency caused by PROS1 gene mutation is an important risk factor for hereditary thrombophilia.

Case Introduction: In this case, we report a 28-year-old male patient who developed a severe pulmonary embolism during his visit.

Design, synthesis and neuroprotective activity of compound derived from Blume and borneol.

Introduction: Traditional Chinese medicine Blume (GEB) possesses properties that soothe the liver and dispel wind. Its constituents exhibit numerous pharmacological properties, including neuroprotective effects, analgesic properties for headache relief, memory enhancement, and others. Borneol enhances drug absorption by traversing the blood-brain barrier, thereby improving its bioavailability and therapeutic efficacy.

Oral microbiome diversity shapes the association between sleep duration and depression.

Background: Emerging research suggests the relationship between the oral microbiome and sleep duration with depression, however, the precise mechanisms by which oral microbial diversity influences the sleep-depression nexus remain to be elucidated.

Methods: We analyzed data from 4,692 participants in the National Health and Nutrition Examination Survey (NHANES), incorporating key demographic variables, oral microbiome diversity metrics, sleep duration, and depression assessment variables. Classical multidimensional scaling facilitated dimensionality reduction, while unsupervised clustering divided participants into groups based on β-diversity dissimilarity matrices.

Challenges in the Development of NK-92 Cells as an Effective Universal Off-the-Shelf Cellular Therapeutic.

The human-derived NK-92 cell-based CAR-NK therapy exhibits inconsistency with overall suboptimal efficacy and rapid in vivo clearance of CAR-NK92 cells in cancer patients. Analysis indicates that although pre-existing IgM in healthy individuals (n = 10) strongly recognizes both NK-92 and CAR-NK92 cells, IgG and IgE do not. However, only a subset of cancer patients (3/8) exhibit strong IgM recognition of these cells, with some (2/8) showing pre-existing IgG recognition.

Case report: Antisynthetase syndrome with positive anti-PL7/SSA/RO52 antibodies.

Background: Antisynthetase syndrome (ASS) is a rare autoimmune disease characterized by the immune system attacking specific synthetase in the body. Due to the difficulty in clinical diagnosis, there is still a lack of effective treatment.

Methods: We report a case of a 50-year-old man who presented with progressive, symmetric limb weakness, starting from the lower limbs and gradually affecting the upper limbs.

Candidaemia: A 9-Year Retrospective Analysis of Epidemiology and Antimicrobial Susceptibility in Tertiary Care Hospitals in Western China.

Purpose: This investigation endeavors to scrutinize the resistance profiles to antifungal agents, alongside the clinical distribution of isolates that yielded positive results in blood cultures at Suining Central Hospital spanning the years 2015 to 2023. The objective is to provide crucial epidemiological insights that may aid in early clinical intervention and judicious deployment of antifungal therapies.

Methods: This retrospective analysis analyses data on 182 different strains with positive clinical blood cultures obtained from the Microbiology Laboratory of Suining Central Hospital over a period of nine consecutive years.

Zinc-Ferricyanide Flow Batteries Operating Stably under -10 °C.

Alkaline ferri/ferro-cyanide-based flow batteries are well suited for energy storage because of their features of high electrochemical activity, good kinetics and low material cost. However, they suffer from low energy density and poor temperature adaptability. The ferri/ferro-cyanide catholyte exhibits low solubility (~0.

Phosphorus vacancies regulation and heterogeneous interfacial engineering of coral-like ZnO/FeCoPv@N-doped carbon hierarchical microspheres to boost overall water splitting.

Vacancy engineering and heterostructure construction are regarded as potent approaches for synergistically boosting hydrogen production in renewable energy conversion. Herein, a selective phosphorization strategy was implemented to fabricate coral-like ZnO/FeCoP@N-doped carbon hierarchical microspheres (ZnO/FeCoP@NCHMS) via only controllably phosphorizing the Co and Fe atoms in a precursor, which was formed by generating ZnCoFe LDH on the surface of a zinc cobalt coordination polymer microsphere. Then, by adopting a reduction treatment for ZnO/FeCoP@NCHMS, the innovative ZnO/FeCoPv@NCHMS with abundant phosphorus vacancies (Pv) was realized.

Research on music therapy from 2013 to 2022: a bibliometric and visualized study.

Background: Music therapy is a rapidly evolving multidisciplinary field. But there has been no research analyzing the latest research status and development trends in this research field from a macro perspective. We aim to identify hotspots, knowledge base, and frontiers in the field of music therapy through bibliometric analysis.

The Association Between Physical Activity and risk for breast cancer in US female adults: A cross-sectional study based on NHANES 2011-2020.

Background: Breast cancer poses a significant threat to women's health worldwide. This study aimed to evaluate the association between various levels of physical activity and the incidence of breast cancer.

Methods: The data for this study were obtained from the National Health and Nutrition Examination Survey (NHANES), spanning 2011 to 2020.