[The relationship between leptin, insulin-like growth factor-1 (IGF-1) and hyperinsulinemia of patients with polycystic ovarian syndrome].

Objective: To identify the effect of IGF-1 and leptin on the hyperinsulinemia (HI) of patients with polycystic ovarian syndrome (PCOS).

Methods: The case-control compared method was applied to the study. Ninety-two PCOS and 92 control subjects were involved in the study.

Mast cells in hemangioma: a double-edged sword.

Hemangioma is a common benign vascular tumor characterized by presentation within the few weeks of life, rapid growth during the first year and spontaneous involution over a period of several years. Mast cells play an important role in immunity and angiogenesis by synthesizing and releasing a group of bioactive mediators, cytokines and chemokines. Previous study confirmed that the number of mast cells is highest during the involuting phase, lower in the involuted phase, and lowest in the proliferating phase.

Immune response: A possible role in the pathophysiology of hemangioma.

Hemangioma is a distinct category of benign vascular tumors characterized by presentation within the first weeks of life, rapid growth during the first year and variable degree of spontaneous involution over a period of several years. Recent research reported that CD8+ T cells in hemangiomas, and the endothelia of hemangioma uniquely expressed leukocyte marker FCgammaRII and myeloid cell marker. Presence of high levels of indoleamine 2,3-dioxygenase in proliferating hemangiomas and significantly decreasing during involution was also confirmed.

[Single nucleotide polymorphism and haplotype in TBX1 gene of patients with conotruncal defects: analysis of 130 cases].

Objective: To investigate the distribution of the single nucleotide polymorphism (SNP) sites in TBX1 gene and the distribution of related haplotypes in the patients with conotruncal defects (CTD) and normal people.

Methods: The genotypes of the 3 selected SNPs: G2857C (rs737868), G2963A (rs28649236), and A6571T (rs28939675) in TBX1 gene were analyzed by PCR-RFLP among 130 patients with CTD and 200 normal people. Contingency table was applied to analyze the frequencies of these SNP genotypes and related alleles.

Inhibition of SHH signaling pathway: molecular treatment strategy of odontogenic keratocyst.

Odontogenic keratocyst (OKC) is a relatively common cystic lesion occurred in the tooth-bearing areas of the jaws. This entity is thought to arise from the dental lamina or its remnant with significant growth capacity and recurrence potential. The Sonic hedgehog (SHH) signaling pathway plays a critical role in tooth development.

A possible hypoxia-induced endothelial proliferation in the pathogenesis of epithelioid hemangioma.

Epithelioid hemangioma is an uncommon benign vascular lesion with distinct clinicopathological characteristics. Histologically, this entity mainly composed of well-formed but often immature vessels lined by epithelioid endothelial cells with prominent chronic inflammatory component. There is considerable controversy whether epithelioid hemangioma is a reactive lesion or a true neoplasm.

A postulated role for transcriptional regulator LMO2 in the proliferation and involution of hemangioma.

Hemangiomas are common benign vascular tumors distinctive for their perinatal presentation, rapid growth during the first year of life and subsequent slowly involution. Many evidences suggest that hemangiomas are angiogenesis dependent entity. Research also indicated that the endothelium of hemangioma shared molecular phenotype with blood cells.

Epithelial expression of SHH signaling pathway in odontogenic tumors.

Sonic hedgehog (SHH) signaling pathway plays a critical role in tooth development. Recent studies indicate that SHH signaling pathway activation occurs both in the odontogenic cyst and ameloblastoma. However, the association of SHH pathway with other subtypes of odontogenic tumor is not well documented.

[Changes in clinical characteristics and interventional modalities of renal artery stenosis in recent years].

Objective: To observe the changes of clinical characteristics and interventional methods of patients with renal artery stenosis in recent 17 years.

Methods: Comparing the clinical characteristics and interventional treatment of patients diagnosed renal artery stenosis by the means of renal angiography in recent 17 years.

Results: The numbers of renal artery stenosis were increasing year by year.

Epithelioid hemangioma in the oral mucosa: a clinicopathological study of seven cases and review of the literature.

To investigate the clinicopathological characteristics and biologic behavior of epithelioid hemangioma in oral mucosa, the clinical features and pathological findings of seven cases of epithelioid hemangioma were reviewed, and all sections were examined by immunohistochemistry. There were 4 males and 3 females ranged from 8 to 65 years (mean 39.7 years).

[Gene expression of transcription regulator LMO 4 in tooth morphogenesis].

Objective: To investigate the expression of transcription regulator LMO4 mRNA in the developing mouse molar and compare the expression pattern of LMO4 with that of Shh signaling molecule.

Methods: Wild-type embryos used in this study (E11.5-P1.

[Exploration on the relationship between thrombosis and stability of coronary atherosclerotic plaque in patients with unstable angina pectoris].

Objective: To evaluate the correlation between thrombosis and stability of atherosclerotic plaque within criminal vessels in patients with unstable angina pectoris (UAP) by coronary angioscopy, to explore the clinical pathological basis for acute coronary syndromes (ACS).

Methods: Sixty-eight patients with UAP were enrolled, the patients with post-infarction angina pectoris and variant angina pectoris were excluded. There were 48 males and 20 females, aged from 40 to 73 (average 62.

[Clinical efficacy and safety of emergent percutaneous coronary intervention for vein-grafts in the patients with acute myocardial infarction: comparison between age groups].

Objective: To evaluate the efficacy and the safety of emergent primary percutaneous coronary intervention (PCI) in the saphenous vein bypass graft (SVBG) of acute myocardial infarction (AMI), and compare the results between aged -patients with non-aged patients.

Methods: Three hundred and nine consecutive AMI patients with culprit SVBG vessels, were analysed, including aged patients 213 cases(>or=70 years old), non-aged patients 96 cases(<70 years old), underwent the emergent primary PCI after confirmed below TIMI III perfusion(TIMI 0-TIMI II) in coronary angiographies. The immediate results and in-hospital outcomes were compared between two groups.

[Distribution and significance of cSNP in KCNA7 gene as a novel NIDDM candidate gene in the population of northeast China].

To investigate the distribution and significance of a coding single nucleotide polymorphism (cSNP) of the novel NIDDM candidate gene,KCNA7 in the population of Northeast China, 97 patients with NIDDM and 141 controls were tested. Genotypes of KCNA7 gene T418M(C/T) were performed by PCR-RFLP, and SSCP was used to detect other unknown variations near the C/T site of KCNA7 gene. As a result, no significant difference was observed in the distribution of genotypes of T418M(C/T) between NIDDM and control group.

[Effect of SH2A gene in cell signal transduction and its subcellular locolization].

Objective: To examine the effect of SH2A gene in cell signal transduction and its subcellular localization.

Methods: RT-PCR method was used to amplify the coding sequence of SH2A gene. Eukaryotic recombined expression vector pcDNA 3.

[Correlation analysis between WNK4 gene and essential hypertension].

Objective: To investigate association of mutation in WNK4 gene with essential hypertension and to analyze the expression of WNK4 gene.

Methods: cSNP in WNK4 gene in a small samples was detected by sequencing, then PCR-RFLP was performed in 98 patients with essential hypertension and 95 control subjects. The expression profile of WNK4 gene was tested by RT-PCR.

[Expression of Shh, Ptc1, Ptc2 mRNA in the cap stage of mouse molar].

Objective: To investigate the expression of Shh and its receptors Ptc1 and Ptc2 mRNA in the cap stage of mouse molar and discuss its role in early tooth morphogenesis.

Methods: The embryonic mouse heads of early tooth development (E10.5 - E15.