The transcriptome of MHV-infected RAW264.7 cells offers an alternative model for macrophage innate immunity research.

Background: Macrophages are the primary innate immune cells encountered by the invading coronaviruses, and their abilities to initiate inflammatory reactions, to maintain the immunity homeostasis by differential polarization, to train the innate immune system by epigenic modification have been reported in laboratory animal research.

Methods: In the current in vitro research, murine macrophage RAW 264.7 cell were infected by mouse hepatitis virus, a coronavirus existed in mouse.

[Effects of human alpha-mannosidase Man2c1 transgene on growth and metastasis of transplanted tumor in mice].

Objective: To study the effect of human alpha-mannosidase Man2c1 transgene on tumor growth and metastasis in mice.

Methods: Hepatoma cell H22 or squamous epithelial carcinoma cell S180 was subcutaneously inoculated into the right armpit of mice (wild type mice and 28#, 35#, and 54# transgenic mice). Tumor size was measured every week.

Inhibition of the alpha-mannosidase Man2c1 gene expression enhances adhesion of Jurkat cells.

Protein N-glycosylation plays very important roles in immunity and alpha-mannosidase is one of the key enzymes in N-glycosylation. This paper reports that inhibition of alpha-mannosidase Man2c1 gene expression enhances adhesion of Jurkat T cells. In comparison to the controls with normal expression of the enzyme, Jurkat cells with the inhibition of Man2c1 gene expression (AS cell) formed larger aggregates in culture, indicating an enhancement of adhesion between the cells.