Effect of ultrasound intervention timing on the formation mechanism and emulsifying properties of chickpea protein isolate-citrus pectin complexes.

This study systematically investigated the effects of ultrasonic treatment timing on the formation and emulsifying properties of chickpea protein isolate (CPI) and citrus pectin (CP) complexes. Results showed that introducing ultrasound during the CPI pre-treatment stage significantly enhanced the emulsifying activity and stability of the complexes. Notably, compared with the independent CPI, the emulsifying activity index of CPIU-CP soared from 0.

Correction: Ci et al. Enhanced Delivery of Imatinib into Vaginal Mucosa via a New Positively Charged Nanocrystal-Loaded in Situ Hydrogel Formulation for Treatment of Cervical Cancer. 2019, , 15.

In the original publication [...

Erratum: Author correction to 'Amino-functionalized poloxamer 407 with both mucoadhesive and thermosensitive properties: Preparation, characterization and application in vaginal drug delivery system' [Acta Pharm Sin B 7 (2017) 593-602].

[This corrects the article DOI: 10.1016/j.apsb.

Effect of low-frequency high-intensity ultrasound (HIU) on the physicochemical properties of chickpea protein.

The aim of this study was to improve the functional properties of chickpea protein for its potential application in the food industry. The effects of low frequency high intensity ultrasound (HIU) at different power (0-300 W) and time (15-30 min) on the rheological properties, gelation, thermal stability, solubility and microstructure of chickpea protein were tested and analyzed. Based on the analysis, it was found that HIU caused the disruption of non-covalent bonds between protein chains leading to the unfolding of chickpea.

Characterization of a Novel High Internal Phase Pickering Emulsions Stabilized by Soy Protein Self-Assembled Gel Particles.

In this paper, a novel high-internal-phase Pickering emulsion (HIPPE) prepared by acid-induced self-assembly SPI gel (A/S-SPIG) was investigated. The steady-state shear test results showed that all HIPPEs were typical shear thinning emulsion, which could form stable emulsion (0.2-1.

[Changes of soil microbial biomass carbon, nitrogen, and enzyme activities in East Dongting Lake wetlands at different water levels].

We analyzed soil quality based on soil microbial characteristics of three different vegetation types in the wetlands of East Dongting Lake, including wetland (CTW), wetland (PAW), and wetland (SBW). The soil microbial biomass carbon (MBC), nitrogen (MBN) and enzyme activities were measured and the key influen-cing factors were analyzed during the normal, flood, and dry periods. The results showed that: 1) The amounts of MBC, MBN, and the activities of invertase and cellulase (except cellulase of dry season) in 0-10 cm were higher than those in 10-20 cm for all wetlands, while the catalase activity showed an opposite pattern.

Evaluation of the diagnostic utility of carbohydrate-deficient transferrin in chronic alcoholism: Results from Southwest China.

Although recent gathered evidence indicates that obtaining the diagnostic value of serum carbohydrate-deficient transferrin might be more useful for identifying alcohol abuse than other widely available biochemical tests; however, its precise value as an indicator of chronic alcoholism is unclear. The main objective is to investigate the diagnostic significance of carbohydrate-deficient transferrin in chronic alcoholism in the Chinese population.In this study, we enrolled (1) 52 physically healthy subjects, (2) 20 patients with nonalcoholic liver disease, and (3) 70 alcoholics.

Inhibitory Effect of PIK-24 on Respiratory Syncytial Virus Entry by Blocking Phosphatidylinositol-3 Kinase Signaling.

Phosphoinositide-3 kinase signaling modulates many cellular processes, including cell survival, proliferation, differentiation, and apoptosis. Currently, it is known that the establishment of respiratory syncytial virus infection requires phosphoinositide-3 kinase signaling. However, the regulatory pattern of phosphoinositide-3 kinase signaling or its corresponding molecular mechanism during respiratory syncytial virus entry remains unclear.

[Characteristics of soil saturated hydraulic conductivity on different positions and their controlling factors of granite collapsing gullies].

Collapsing gully is a common phenomenon of hydraulic-gravity combined soil erosion in granite hilly area of south China. The study aimed to explore the relationship between soil hydraulics pro-perties and erosion mechanism and the intrinsic controlling factors. The active, semi-stable, and stable types of granite collapsing gullies in southeastern Guangxi were selected to examine the spatial variation of soil saturated hydraulic conductivity and identify the influencing factors.

[Screening of reference genes based on real-time quantitative PCR analysis in Monochasma savatieri].

Monochasma savatieri belongs to Scrophulariaceae family. It is a facultative parasitic plant distributed in southern China that has been found in Jiangxi, Guangdong, and Fujian provinces. Previous studies on this plant have focused on its chemical constituents and pharmacological effects.

Effect of high pressure homogenization treatment on the rheological properties of citrus peel fiber/corn oil emulsion.

Background: Citrus fiber is a main component in the peel of citrus and contains natural dietary fiber. It is often used as a functional additive to improve the texture or nutritional property of food. It is also widely used to reduce the content of absorbable fat in sausages and other meat products, and to improve food stability as an emulsifier.

Endogenous hydrogen sulfide and ERK1/2-STAT3 signaling pathway may participate in the association between homocysteine and hypertension.

Background: Homocysteine (Hcy) is a risk factor for hypertension, although the mechanisms are poorly understood.

Methods: We first explored the relationship between Hcy levels and blood pressure (BP) by analyzing the clinical data of primary hypertensive patients admitted to our hospital. Secondly, we explored a rat model to study the effect of Hcy on blood pressure and the role of HS.

Association between potentially functional polymorphisms of chemokine family members and the survival of esophageal cancer patients in a Chinese population.

The chemokine family plays an important role in the growth, invasion, and metastasis of tumors. However, most studies have only focused on a few genes or a few gene loci, and thus could not reveal the associations between functional polymorphisms of chemokine family members and tumor progression. This study aimed to determine the associations between single nucleotide polymorphisms (SNPs) of chemokine family members and the prognosis of esophageal cancer (EC).

Discovery and evaluation of novel nitrodihydroimidazooxazoles as promising anti-tuberculosis agents.

New analogues of antitubercular drug Delamanid were prepared, seeking drug candidates with enhanced aqueous solubility and high efficacy. The strategy involved replacement of phenoxy linker proximal to the 2-nitroimidazooxazole of Delamanid by piperidine fused 5 or 6-membered ring heterocycles (ring A). The new compounds were all more hydrophilic than Delamanid, and several class of analogues showed remarkable activities against M.

Enhanced Delivery of Imatinib into Vaginal Mucosa via a New Positively Charged Nanocrystal-Loaded in Situ Hydrogel Formulation for Treatment of Cervical Cancer.

The present study was carried out to investigate the potential of cationic functionalization on imatinib nanocrystals to improve the mucoadhesiveness and, thus, delivery to the lesion of cervicovaginal tumors. Amino-group-functionalized imatinib nanocrystals (NC@PDA-NH₂) were prepared with near-spheroid shape, nanoscale size distribution, positive zeta potential, and relatively high drug content with the aid of the polydopamine-coating technique. Efficient interaction between NC@PDA-NH₂ and mucin was proven by mucin adsorption which was related to the positive zeta-potential value of NC@PDA-NH₂ and the change in the size distribution on mixing of NC@PDA-NH₂ and mucin.

RGD-modified PEGylated paclitaxel nanocrystals with enhanced stability and tumor-targeting capability.

Nanocrystals has been constructed for insoluble drugs as a novel type of nanoscale drug delivery systems with high drug loading. How to prepare nanocrystals with good stability and tumor targeting capability is still challenging. This study was to modify paclitaxel nanocrystals with polyethylene glycol (PEG) for stabilization and RGD peptide for tumor targeting.

Construction of a recombinant eukaryotic expression vector containing gene and its expression in colon cancer cells.

Introduction: Dynamin 3 (DNM3) is a large GTPase that possesses mechanochemical properties and has been shown to be involved in malignancies. However, most studies about DNM3 are observational, and knowledge of the precise molecular mechanism of DNM3 remains limited.

Materials And Methods: We constructed a PCDH-CMV-MCS-EF1a-GFP-Puro-DNM3 recombinant eukaryotic expression vector, which was then transfected into SW620 and LoVo cells.

[The Influence of Transferrin Gene Polymorphism on Measuring Carbohydrate Deficient Transferrin by Capillary Electrophoresis].

Objective: To determine the influence of isoforms of transferrin (Tf) on the detection of serum carbohydrate-deficient transferrin (CDT) by capillary electrophoresis (CE).

Methods: A total of 51,17,and 65 serum samples were collected from the healthy participants,the patients with non-alcoholic liver diseases,and the patients with alcoholic liver diseases,respectively. Serum CDT was measured by CE.

Determination of Arsenic Species in from Major Habitats in China by HPLC-ICP-MS and the Edible Hazard Assessment.

This study sought to determine the concentration and distribution of arsenic (As) species in (), and to assess its edible hazard for long term consumption. The total arsenic concentrations, measured through inductively coupled plasma mass spectrometry (ICP-MS), ranged from 4.00 mg/kg to 5.

Effect of Bamboo Flour Grafted Lactide on the Interfacial Compatibility of Polylactic Acid/Bamboo Flour Composites.

Bamboo flour (BF) was grafted onto lactide (LA) in the molten state using stannous octoate as a catalyst to form BF-g-LA. Then, polylactic acid (PLA) was blended with BF (PLA/BF, 85/15 wt %) to prepare PLA/BF/BF-g-LA composites using BF-g-LA as a compatibilizer. The grafting rate of BF was characterized using infrared testing and elemental analysis.

[Effects of TRAM-34 on Proliferation and Invasion of Leukemia Cell Line HL-60].

Objective: To investigate the effects of KCa3.1 channel inhibitor TRAM-34 on the proliferation and invasion of leukemia cell line HL-60.

Methods: HL-60 cells at logarithmic growth phase exposed to TRAM-34 at the final concentration of 25, 50, 75 and 100 nmol/L were used as experimental group.

Sulfonate-modified phenylboronic acid-rich nanoparticles as a novel mucoadhesive drug delivery system for vaginal administration of protein therapeutics: improved stability, mucin-dependent release and effective intravaginal placement.

Effective interaction between mucoadhesive drug delivery systems and mucin is the basis of effective local placement of drugs to play its therapeutic role after mucosal administration including vaginal use, which especially requires prolonged drug presence for the treatment of gynecological infectious diseases. Our previous report on phenylboronic acid-rich nanoparticles (PBNPs) demonstrated their strong interaction with mucin and mucin-sensitive release profiles of the model protein therapeutics interferon (IFN) in vitro, but their poor stability and obvious tendency to aggregate over time severely limited future application. In this study, sulfonate-modified PBNPs (PBNP-S) were designed as a stable mucoadhesive drug delivery system where the negative charges conferred by sulfonate groups prevented aggregation of nanoparticles and the phenylboronic acid groups ensured effective interaction with mucin over a wide pH range.

The glycosylation of the extracellular loop of β2 subunits diversifies functional phenotypes of BK Channels.

Large-conductance Ca- and voltage-activated potassium (MaxiK or BK) channels are composed of a pore-forming α subunit (Slo) and 4 types of auxiliary β subunits or just a pore-forming α subunit. Although multiple N-linked glycosylation sites in the extracellular loop of β subunits have been identified, very little is known about how glycosylation influences the structure and function of BK channels. Using a combination of site-directed mutagenesis, western blot and patch-clamp recordings, we demonstrated that 3 sites in the extracellular loop of β2 subunit are N-glycosylated (N-X-T/S at N88, N96 and N119).

[Estimations of application dosage and greenhouse gas emission of chemical pesticides in staple crops in China.].

Chemical pesticides play an important role in improving crop yield in modern agriculture. However, commonly overuse of pesticide in China leads to serious environmental problems and food safety hazards. Based on a national questionnaire survey of farmers across China in 2012, the situation of pesticide applications to rice, wheat, and corn in 2011, and their corresponding greenhouse gas (GHG) emissions were investigated.

[Evolution of Dissolved Organic Matter Properties in a Constructed Wetland of Xiao River, Hebei].

The evolution of water DOC and COD, and the source, chemical structure, humification degree and redox of dissolved organic matter (DOM) in a constructed wetland of Xiao River, Hebei, was investigated by 3D excitation--emission matrix fluorescence spectroscopy coupled with ultraviolet spectroscopy and chemical reduction, in order to explore the geochemical processes and environmental effects of DOM. Although DOC contributes at least 60% to COD, its decrease in the constructed wetland is mainly caused by the more extensive degradation of elements N, H, S, and P than C in DOM, and 65% is contributed from the former. DOM is mainly consisted of microbial products based on proxies f470/520 and BIX, indicating that DOM in water is apparently affected by microbial degradation.