Antimicrobial peptide Hs02 with rapid bactericidal, anti-biofilm, and anti-inflammatory activity against carbapenem-resistant and .

Unlabelled: Carbapenem-resistant (CRKP) and (CREC) are frequently detected in clinical settings, restricting the use of carbapenems. Therefore, there is an urgent need for new antimicrobial strategies to address infections caused by CRKP and CREC. This study investigated the antibacterial, anti-biofilm, and anti-inflammatory effects of the cationic antimicrobial peptide Hs02, along with its potential antimicrobial mechanisms against CRKP and CREC.

A Klebsiella-phage cocktail to broaden the host range and delay bacteriophage resistance both in vitro and in vivo.

Bacteriophages (phages), viruses capable of infecting and lysing bacteria, are a promising alternative for treating infections from hypervirulent, antibiotic-resistant pathogens like Klebsiella pneumoniae, though narrow host range and phage resistance remain challenges. In this study, the hypervirulent K. pneumoniae NTUH-K2044 was used to purify phage ΦK2044, while two ΦK2044-resistant strains were used to purify two further phages: ΦKR1, and ΦKR8 from hospital sewage.

Synergy of bacteriophage depolymerase with host immunity rescues sepsis mice infected with hypervirulent of capsule type K2.

The hypervirulent (hvKp) with K1 and K2 capsular types causes liver abscess, pneumonia, sepsis, and invasive infections with high lethality. The presence of capsular polysaccharide (CPS) resists phagocytic engulfment and contributes to excessive inflammatory responses. Bacteriophage depolymerases can specifically target bacterial CPS, neutralizing its defense.

Coexistence of plasmid-mediated , , and in .

is a potential pathogen that has not been studied comprehensively. The emergence of multidrug-resistant (MDR) , specifically strains resistant to tigecycline and carbapenem, presents a significant challenge to clinical treatment. This investigation aimed to characterize MDR strain FK8966, co-carrying , , and by plasmids.

Plumbagin enhances antimicrobial and anti-biofilm capacities of chlorhexidine against clinical while reducing resistance mutations.

With the widespread misuse of disinfectants, the clinical susceptibility of () to chlorhexidine (CHX) has gradually diminished, posing significant challenges to clinical disinfection and infection control. employs overexpression of efflux pumps and the formation of thick biofilms to evade the lethal effects of CHX. Plumbagin (PLU) is a natural plant extract that enhances membrane permeability and reduces proton motive force.

Host immunity involvement in the outcome of phage therapy against hypervirulent infections.

Highly encapsulated hypervirulent (hvKp) causes severe infections. Bacteriophage therapy, an antibiotic alternative, effectively treats bacterial infections. Phage φFK1979 encoding polysaccharide depolymerases can target and disarm the capsule of hvKp FK1979, showing promise against FK1979 infection.

Azomycin Orchestrate Colistin-Resistant Complex's Colistin Resistance Reversal In Vitro and In Vivo.

The complex (ECC) is a group of nosocomial pathogens that pose a challenge in clinical treatment due to its intrinsic resistance and the ability to rapidly acquire resistance. Colistin was reconsidered as a last-resort antibiotic for combating multidrug-resistant ECC. However, the persistent emergence of colistin-resistant (COL-R) pathogens impedes its clinical efficacy, and novel treatment options are urgently needed.