Genomic and Transcriptomic Analysis of Pea ( L.) Breeding Line 'Triumph' with High Symbiotic Responsivity.

Pea ( L.), like most legumes, forms mutualistic symbioses with nodule bacteria and arbuscular mycorrhizal (AM) fungi. The positive effect of inoculation is partially determined by the plant genotype; thus, pea varieties with high and low symbiotic responsivity have been described, but the molecular genetic basis of this trait remains unknown.

A variable gene family encoding nodule-specific cysteine-rich peptides in pea ( L.).

Various legume plants form root nodules in which symbiotic bacteria (rhizobia) fix atmospheric nitrogen after differentiation into a symbiotic form named bacteroids. In some legume species, bacteroid differentiation is promoted by defensin-like nodule-specific cysteine-rich (NCR) peptides. NCR peptides have best been studied in the model legume Gaertn.

Metabolic alterations in pea leaves during arbuscular mycorrhiza development.

Arbuscular mycorrhiza (AM) is known to be a mutually beneficial plant-fungal symbiosis; however, the effect of mycorrhization is heavily dependent on multiple biotic and abiotic factors. Therefore, for the proper employment of such plant-fungal symbiotic systems in agriculture, a detailed understanding of the molecular basis of the plant developmental response to mycorrhization is needed. The aim of this work was to uncover the physiological and metabolic alterations in pea ( L.

Mapping-by-sequencing using NGS-based 3'-MACE-Seq reveals a new mutant allele of the essential nodulation gene () in pea ( L.).

Large collections of pea symbiotic mutants were accumulated in the 1990s, but the causal genes for a large portion of the mutations are still not identified due to the complexity of the task. We applied a Mapping-by-Sequencing approach including Bulk Segregant Analysis and Massive Analysis of cDNA Ends (MACE-Seq) sequencing technology for genetic mapping the gene of pea which controls the formation of symbioses with both nodule bacteria and arbuscular-mycorrhizal fungi. For mapping we developed an -population from the cross between pea line N24 carrying the mutant allele of and the wild type NGB1238 (=JI0073) line.

s-dePooler: determination of polymorphism carriers from overlapping DNA pools.

Background: Samples pooling is a method widely used in studies to reduce costs and labour. DNA sample pooling combined with massive parallel sequencing is a powerful tool for discovering DNA variants (polymorphisms) in large analysing populations, which is the base of such research fields as Genome-Wide Association Studies, evolutionary and population studies, etc. Usage of overlapping pools where each sample is present in multiple pools can enhance the accuracy of polymorphism detection and allow identifying carriers of rare-variants.

Selection Signatures in the First Exon of Paralogous Receptor Kinase Genes from the Region of the L. Genome.

During the initial step of the symbiosis between legumes (Fabaceae) and nitrogen-fixing bacteria (rhizobia), the bacterial signal molecule known as the Nod factor (nodulation factor) is recognized by plant LysM motif-containing receptor-like kinases (LysM-RLKs). The fifth chromosome of barrel medic ( Gaertn.) contains a cluster of paralogous LysM-RLK genes, one of which is known to participate in symbiosis.

Pea Marker Database (PMD) - A new online database combining known pea (Pisum sativum L.) gene-based markers.

Pea (Pisum sativum L.) is the oldest model object of plant genetics and one of the most agriculturally important legumes in the world. Since the pea genome has not been sequenced yet, identification of genes responsible for mutant phenotypes or desirable agricultural traits is usually performed via genetic mapping followed by candidate gene search.

Draft genome of the strain RCAM1026 bv. .

bv. RCAM1026 is a strain first isolated in 1964 from nodules of "Ramensky 77" cultivar of garden pea ( L.) now routinely used as a model strain in inoculation experiments on pea.

Massive Analysis of cDNA Ends (MACE) for transcript-based marker design in pea ( L.).

Aimed at gene-based markers design, we generated and analyzed transcriptome sequencing datasets for six pea ( L.) genetic lines that have not previously been massively genotyped. Five cDNA libraries obtained from nodules or nodulated roots of genetic lines Finale, Frisson, Sparkle, Sprint-2 and NGB1238 were sequenced using a versatile 3'-RNA-seq protocol called MACE (Massive Analysis of cDNA Ends).

De Novo Assembly of the Pea (Pisum sativum L.) Nodule Transcriptome.

The large size and complexity of the garden pea (Pisum sativum L.) genome hamper its sequencing and the discovery of pea gene resources. Although transcriptome sequencing provides extensive information about expressed genes, some tissue-specific transcripts can only be identified from particular organs under appropriate conditions.

[The Principle of Genome Complementarity in the Enhancement of Plant Adaptive Capacities].

In the present work, the potential for the enhancement of the adaptive capacity of microbe-plant systems (MPSs) through the integration of the symbiosis partners' genomes is considered on the example of different types of symbiotic relationships. The accumulated data on the genetic control of interactions for both the plant and microbe, which are discussed in the paper with respect to signaling genes, suggest that it is the complementarity of genetic determinants that underlies the successful formation of MPSs. A eukaryotic genome with limited information content, which is stable throughout a generation, is complemented by a virtually unlimited prokaryotic metagenome.

[Regulatory genes of garden pea (Pisum sativum L.) controlling the development of nitrogen-fixing nodules and arbuscular mycorrhiza: a review of basic and applied aspects].

The review sums up the long experience of the authors and other researchers in studying the genetic system of garden pea (Pisum sativum L.), which controls sthe development of nitrogen-fixing symbiosis and arbuscular mycorrhiza. A justified phenotypic classification of pea mutants is presented.

[Rearrangement of the hemodynamic mechanisms of orthostatic tolerance of humans on long-term space missions].

Orthostatic tolerance (OT) was evaluated in 25 cosmonauts using the traditional standing test before and after space fights of about six months in length; 15 cosmonauts of this group were also subjected to ultrasonic investigation during the lower body negative pressure test before, during and after long-term SF. Efficiency of the OT vascular mechanisms was gradually falling and stabilized at the end of six-month SF at a level significantly below pre-flight level. Post-flight OT recovery took longer time as compared with short-term SF suggesting more profound shifts in the vascular control system.