Effects of naturally aged microplastics on arsenic and cadmium accumulation in lettuce: Insights into rhizosphere microecology.

Naturally aged microplastics (NAMPs) are commonly found in farmland soils contaminated with heavy metals (HMs), such as arsenic (As) and cadmium (Cd); yet their combined effects on soil-plant ecosystems remain poorly understood. In this study, we investigated the toxic effects of NAMPs and As-Cd on lettuce, considering the influence of earthworm activity, and examined changes in As-Cd bioavailability in the rhizosphere. Four experimental systems were established: soil-only, soil-lettuce, soil-earthworms, and soil-lettuce-earthworms systems, with four NAMPs concentrations (0, 0.

Source-specific soil heavy metal risk assessment in arsenic waste mine site of Yunnan: Integrating environmental and biological factors.

This study quantified heavy metal (HM) pollution risks in mining site soils to provide targeted solutions for environmental remediation. Focusing on As waste mine sites in Yunnan, we utilised multiple indices and a positive matrix factorisation model to assess and quantify ecological health risks. Our ecological risk assessment distinguished between environmental and biological factors.

Adaptation of rhizobacterial and endophytic communities in to long-term organic and chemical fertilization.

Introduction: Organic fertilizers (OF) are crucial for enhancing soil quality and fostering plant growth, offering a more eco-friendly and enduring solution compared to chemical fertilizers (CF). However, few studies have systematically analyzed the effects of OF/CF on root microbiome of medicinal plants, especially in combination with active ingredients.

Methods: In this study, we investigated the composition and function of bacteria and fungi in the rhizosphere or within the root of traditional Chinese medicinal plants, (Huajuhong), which were treated with OF or CF over 1, 3, and 5 years (starting from 2018).

Enhanced growth of wheat in contaminated fields via synthetic microbiome as revealed by genome-scale metabolic modeling.

The relationship between plants and soil microbial communities is complex and subtle, with microbes playing a crucial role in plant growth. Autochthonous bioaugmentation and nutrient biostimulation are promising bioremediation methods for herbicides in contaminated agricultural soils, but how microbes interact to promote biodegradation and plant growth on barren fields, especially in response to the treatment of the herbicide bromoxynil after wheat seedlings, remains poorly understood. In this study, we explored the microbial community reassembly process from the three-leaf stage to the tillering stage of wheat and put forward the idea of using the overlapping results of three methods (network Zi-Pi analysis, LEfSe analysis, and Random Forest analysis) as keystones for the simplification and optimization of key microbial species in the soil.

Tessaracoccus lacteus sp. nov., Isolated from the Sludge of a Wastewater Treatment Plant.

A bacterium, designated strain T21, that is non-motile, rod-shaped, and formed pale white colonies, was isolated from the sludge of a wastewater treatment plant's secondary sedimentation tank in China. Strain T21 could grow at 20-40 °C (optimum growth at 30 °C), pH 3.0-10.

Engineering natural microbiomes toward enhanced bioremediation by microbiome modeling.

Engineering natural microbiomes for biotechnological applications remains challenging, as metabolic interactions within microbiomes are largely unknown, and practical principles and tools for microbiome engineering are still lacking. Here, we present a combinatory top-down and bottom-up framework to engineer natural microbiomes for the construction of function-enhanced synthetic microbiomes. We show that application of herbicide and herbicide-degrader inoculation drives a convergent succession of different natural microbiomes toward functional microbiomes (e.

Description and Genomic Characteristics of Diaphorobacter limosus sp. nov., Isolated from a Sewage-Treatment Plant.

A Gram-stain-negative, rod-shaped, non-motile, catalase-positive, denitrifying bacterium, designated strain Y-1, was isolated from an aeration tank of a sewage treatment plant in China and characterized using polyphasic taxonomic approaches. Strain Y-1 could grow at 10-37 °C (optimum 25 °C), at pH 5.0-10.

Modified nano zero-valent iron coupling microorganisms to degrade BDE-209: Degradation pathways and microbial responses.

Polybrominated diphenyl ethers (PBDEs) in soil and groundwater have garnered considerable attention owing to the significant bioaccumulation potential and toxicity. Currently, the coupling treatment method of nano zero-valent iron (nZVI) with dehalogenation microorganisms is a research hotspot in the field of PBDE degradation. In this study, various systems were established within anaerobic environments, including the nZVI-only system, microorganism-only system, and the nZVI + microorganisms system.

Interspecies Metabolic Interactions in a Synergistic Consortium Drive Efficient Degradation of the Herbicide Bromoxynil Octanoate.

Microbial communities play vital roles in biogeochemical cycles, allowing biodegradation of a wide range of pollutants. Although many studies have shown the importance of interspecies interactions on activities of communities, fully elucidating the complex interactions in microbial communities is still challenging. Here, we isolated a consortium containing two bacterial strains ( sp.

Comparative Genomic Analysis of Pseudoxanthomonas sp. X-1, a Bromoxynil Octanoate-Degrading Bacterium, and Its Related Type Strains.

Most Pseudoxanthomonas species described have been derived from water, plants, or contaminated soils. Here, a strain Pseudoxanthomonas sp. X-1 isolated from bromoxynil octanoate (BO)-contaminated soil is presented.

Bioaugmentation of acetamiprid-contaminated soil with Pigmentiphaga sp. strain D-2 and its effect on the soil microbial community.

Bioaugmentation, a strategy based on microbiome engineering, has been proposed for bioremediation of pollutant-contaminated environments. However, the complex microbiome engineering processes for soil bioaugmentation, involving interactions among the exogenous inoculum, soil environment, and indigenous microbial microbiome, remain largely unknown. Acetamiprid is a widely used neonicotinoid insecticide which has caused environmental contaminations.

Oleisolibacter albus gen. nov., sp. nov., isolated from an oil-contaminated soil.

A Gram-stain-negative, aerobic, flagellated, rod-shaped bacterium, designated strain NAU-10, was isolated from an oil-contaminated soil collected in PR China. Strain NAU-10 could grow at 10-42 °C (optimum, 30 °C), pH 5.0-9.

Genome Analysis of Haplotype D of Liberibacter Solanacearum.

Liberibacter solanacearum (Lso) haplotype D (LsoD) is a suspected bacterial pathogen, spread by the phloem-feeding psyllid Hodkinson and found to infect carrot plants throughout the Mediterranean. Haplotype D is one of six haplotypes of Lso that each have specific and overlapping host preferences, disease symptoms, and psyllid vectors. Genotyping of rRNA genes has allowed for tracking the haplotype diversity of Lso and genome sequencing of several haplotypes has been performed to advance a comprehensive understanding of Lso diseases and of the phylogenetic relationships among the haplotypes.

Modeling trophic dependencies and exchanges among insects' bacterial symbionts in a host-simulated environment.

Background: Individual organisms are linked to their communities and ecosystems via metabolic activities. Metabolic exchanges and co-dependencies have long been suggested to have a pivotal role in determining community structure. In phloem-feeding insects such metabolic interactions with bacteria enable complementation of their deprived nutrition.

Genome Sequence of " Carsonella ruddii" Strain BT from the Psyllid .

The genome of " Carsonella ruddii" strain BT from in Israel was sequenced. The full-length genome is 173,904 bp long and has a G+C content of 14.6%, with 224 predicted open reading frames (ORFs) and 30 RNAs.

Response of soil bacterial communities to lead and zinc pollution revealed by Illumina MiSeq sequencing investigation.

Soil provides a critical environment for microbial community development. However, microorganisms may be sensitive to substances such as heavy metals (HMs), which are common soil contaminants. This study investigated bacterial communities using 16S ribosomal RNA (rRNA) gene fragment sequencing in geographic regions with and without HM pollution to elucidate the effects of soil properties and HMs on bacterial communities.

Huakuichenia soli gen. nov., sp. nov., a new member of the family Microbacteriaceae, isolated from contaminated soil.

A novel Gram-stain-positive, non-motile, rod-shaped bacterial strain designated LIP-1T was isolated from the contaminated soil of a pesticide factory in Xinyi, China, was investigated for its taxonomic allocation by a polyphasic approach. Cell growth occurred at 16-37 °C (optimum, 30 °C), in the presence of 0-2.0 % (w/v) NaCl (optimum, 0 %) and at pH 6.

Reductive dehalogenation of 3,5-dibromo-4-hydroxybenzoate by an aerobic strain of Delftia sp. EOB-17.

Objectives: To confirm the reductive dehalogenation ability of the aerobic strain of Delftia sp. EOB-17, finding more evidences to support the hypothesis that reductive dehalogenation may occur extensively in aerobic bacteria.

Results: Delftia sp.