Detection of casein kinase II by aggregation-induced emission.

A novel aggregation-induced emission (AIE) probe comprised of a hydrophilic protein kinase specific peptide and a hydrophobic tetraphenylethene (TPE) unit was synthesized through click reaction. The prepared TPE-peptide probe could be completely degraded by carboxypeptidase Y (CPY) to release hydrophobic TPE part, which aggregated in buffer solution and showed strong TPE emission. In the presence of casein kinase (CKII), the phosphorylation of peptide prevented the complete degradation by CPY producing the nonemissive probe.

A versatile cathodic "signal-on" photoelectrochemical platform based on a dual-signal amplification strategy.

Novel cathodic photoelectrochemical (PEC) aptasensors for sensitive and selective determination of thrombin and Pb were developed based on a new dual-signal amplification strategy. The presence of gold nanoparticles (AuNPs) could quench the PEC signal of bismuth oxyiodide (BiOI). At the same time, the redox moiety G-quadruplex/hemin or ferrocene (Fc) was found to enhance the PEC signal of BiOI.

Application of a cation-exchange reaction of CuS nanoparticles and fluorescent copper nanoparticles in a DNA biosensor.

A novel detection method based on the cation-exchange reaction of CuS nanoparticles (CuS NPs) combined with poly T-templated fluorescent Cu nanoparticles (Cu NPs) was developed. First, CuS NPs-magnetic bead conjugates were prepared through the hybridization of DNA. Competition with target DNA resulted in the release of CuS NPs, and exonuclease III catalysis could lead to recycling of the target DNA.