Heating promoted super sensitive electrochemical detection of p53 gene based on alkaline phosphatase and nicking endonuclease Nt.BstNBI-assisted target recycling amplification strategy at heated gold disk electrode.

An ultrasensitive electrochemical biosensor for detecting p53 gene was fabricated based on heated gold disk electrode coupling with endonuclease Nt.BstNBI-assisted target recycle amplification and alkaline phosphatase (ALP)-based electrocatalytic signal amplification. For biosensor assembling, biotinylated ssDNA capture probes were first immobilized on heated Au disk electrode (HAuDE), then combined with streptavidin-alkaline phosphatase (SA-ALP) by biotin-SA interaction.

Polyacylated Anthocyanins Derived from Red Radishes Protect Vascular Endothelial Cells Against Palmitic Acid-Induced Apoptosis via the p38 MAPK Pathway.

Palmitic acid (PA), a widely consumed saturated fat, is known to induce the apoptosis of vascular endothelial cells. This study examined the protective effect of anthocyanin from red radish (ARR), which has been shown to protect the cardiovascular system and is rich in polyacylated pelargonidin (P) glycosides, on PA-treated SV 40 transfected aortic rat endothelial cells (SVAREC). In all, 22 distinct anthocyanins were identified in the ARR via ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry, the most abundant of which were pelargonidin-3-(p-coumaroyl)diglucoside-5-glucoside (31.

Temperature-Controllable Electrodes with a One-Parameter Calibration.

Electrically heated electrodes have been applied for various chemical and biological sensors. However, previous electrically heated electrodes, including microwires and microdiscs, are usually small and often suffer from the requirement of frequent calibrations of the electrode surface temperature ( T) at different environment temperatures. Here, we fabricate a temperature-controllable disk electrode (TCDE) with a conventional size (3-5 mm in diameter).

Heating enhanced sensitive and selective electrochemical detection of Hg based on T-Hg-T structure and exonuclease III-assisted target recycling amplification strategy at heated gold disk electrode.

A sensitive and selective electrochemical Hg sensor was developed based on T-Hg-T structure and exonuclease (Exo) III -assisted target recycling amplification at heated gold disk electrode (HAuDE). First, a DNA signal probe P1 was for the first time designed and labeled with ferrocene (Fc) near the attached SH-5'-end, so as to shorten the distance between Fc and the electrode and enhance the initial current of Fc compared with that labeled at the 3'-end far from the electrode. Then the signal amplification was achieved by Exo III-assisted Hg recycling.