Cytokinins enhance the metabolic activity of in vitro-grown catmint (Nepeta nuda L.).

The phytohormones cytokinins are essential mediators of developmental and environmental signaling, primarily during cell division and endophytic interactions, among other processes. Considering the limited understanding of the regulatory mechanisms that affect the growth and bioactivity of the medicinal plant Nepeta nuda (Lamiaceae), our study aimed to explore how cytokinins influence the plant's metabolic status. Exogenous administration of active cytokinin forms on in vitro N.

Exploring the Phytochemical Composition and Biological Potential of Balkan Endemic Species Griseb.

Griseb. is a Balkan endemic species listed in The Red Data Book of Bulgaria with the conservation status "endangered". Successful micropropagation was achieved on MS medium supplemented with 1.

Uncovering the Interrelation between Metabolite Profiles and Bioactivity of In Vitro- and Wild-Grown Catmint ( L.).

L. is a medicinal plant enriched with secondary metabolites serving to attract pollinators and deter herbivores. Phenolics and iridoids of have been extensively investigated because of their beneficial impacts on human health.

Jewel Orchid's Biology and Physiological Response to Aquaponic Water as a Potential Fertilizer.

is commonly known as a jewel orchid due to its variegated leaves. Easy maintenance of the orchid allows it to be used as a test system for various fertilizers and nutrient sources, including aquaponic water (AW). First, we applied DNA barcoding to assess the taxonomic identity of this terrestrial orchid and to construct phylogenetic trees.

Catmint ( L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions.

(catmint; Lamiaceae) is a perennial medicinal plant with a wide geographic distribution in Europe and Asia. This study first characterized the taxonomic position of using DNA barcoding technology. Since medicinal plants are rich in secondary metabolites contributing to their adaptive immune response, we explored the metabolic adjustment operating under variable environments.

Biological Activity and NMR-Fingerprinting of Balkan Endemic Species Davidov.

Davidov is a Balkan endemic species distributed in Bulgaria, Greece, and Turkey. In Bulgaria, it is classified as "rare" and is under the protection of the Bulgarian biodiversity law. The aim of our study was to develop an efficient protocol for ex situ conservation of and to perform comparative NMR-based metabolite profiling and bioactivity assays of extracts from in situ grown, in vitro cultivated, and ex vitro acclimated plants.

In Vitro Multiplication and NMR Fingerprinting of Rare M. Bieb.

Micropropagation of rare M. Bieb. was achieved by successful in vitro cultivation of mono-nodal segments on MS medium supplemented with 1.

Green (cell) factories for advanced production of plant secondary metabolites.

For centuries plants have been intensively utilized as reliable sources of food, flavoring, agrochemical and pharmaceutical ingredients. However, plant natural habitats are being rapidly lost due to climate change and agriculture. Plant biotechnology offers a sustainable method for the bioproduction of plant secondary metabolites using plant systems.

Transformed Root Culture: From Genetic Transformation to NMR-Based Metabolomics.

Hairy root (HR) culture is considered as "green factory" for mass production of bioactive molecules with pharmaceutical relevance. As such, HR culture has an immense potential as a valuable platform to elucidate biosynthetic pathways and physiological processes, generate recombinant therapeutic proteins, assist molecular breeding, and enhance phytoremediation efforts. However, some plant species appear recalcitrant to the classical Agrobacterium rhizogenes transformation techniques.

Genetic transformation of rare Verbascum eriophorum Godr. plants and metabolic alterations revealed by NMR-based metabolomics.

Objectives: To develop a protocol to transform Verbascum eriophorum and to study the metabolic differences between mother plants and hairy root culture by applying NMR and processing the datasets with chemometric tools.

Results: Verbascum eriophorum is a rare species with restricted distribution, which is poorly studied. Agrobacterium rhizogenes-mediated genetic transformation of V.

Changes in the functional characteristics of tumor and normal cells after treatment with extracts of white dead-nettle.

L. is a perennial herb widely used in folk medicine. It possesses a wide spectrum of therapeutic activities (anti-inflammatory, astringent, antiseptic, antibiotic, antispasmodic, antioxidant and anti-proliferative).

Influence of cryopreservation on the antioxidative activity of cultivated species.

Antioxidative activity of two cultivated species - Boiss. and Fr. - was estimated after cryopreservation.

Involvement of ethylene and nitric oxide in cell death in mastoparan-treated unicellular alga Chlamydomonas reinhardtii.

This work demonstrates a contribution of ethylene and NO (nitric oxide) in MP (mastoparan)-induced cell death in the green algae Chlamydomonas reinhardtii. Following MP treatment, C. reinhardtii showed massive cell death, expressing morphological features of PCD (programmed cell death).