Publications by authors named "Zhenwei Pan"

In the healing process of myocardial infarction, cardiac fibroblasts are activated to produce collagen, leading to adverse remodeling and heart failure. Our previous study showed that ASPP1 promotes cardiomyocyte apoptosis by enhancing the nuclear trafficking of p53. We thus explored the influence of ASPP1 on myocardial fibrosis and the underlying mechanisms.

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  • * Researchers utilized a fine-mapping approach blending data from a diverse genome-wide association study and expression analysis from different ancestry groups to identify 14 potential causal genes linked to AF.
  • * By silencing a major AF-related gene using CRISPR in heart cells, the study revealed disruptions in pathways crucial for heart tissue and conduction system development.
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The risk for suffering immune checkpoint inhibitors (ICIs)-associated myocarditis increases in patients with pre-existing conditions and the mechanisms remain to be clarified. Spatial transcriptomics, single-cell RNA sequencing, and flow cytometry are used to decipher how anti-cytotoxic T lymphocyte antigen-4 m2a antibody (anti-CTLA-4 m2a antibody) aggravated cardiac injury in experimental autoimmune myocarditis (EAM) mice. It is found that anti-CTLA-4 m2a antibody increases cardiac fibroblast-derived C-X-C motif chemokine ligand 1 (Cxcl1), which promots neutrophil infiltration to the myocarditic zones (MZs) of EAM mice via enhanced Cxcl1-Cxcr2 chemotaxis.

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Cardiac fibrosis is a pathological scarring process that impairs cardiac function. N-acetyltransferase 10 (Nat10) is recently identified as the key enzyme for the N4-acetylcytidine (ac4C) modification of mRNAs. In this study, we investigated the role of Nat10 in cardiac fibrosis following myocardial infarction (MI) and the related mechanisms.

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  • G protein-coupled receptors, especially galanin receptors (GALRs), are significant in understanding atrial fibrillation (AF), with spexin identified as a unique ligand affecting these receptors.
  • Research involving genetically modified mice showed that the absence of spexin increased the likelihood of AF and disrupted potassium currents and calcium handling in heart cells.
  • The study concluded that spexin lowers AF risk by inhibiting the phosphorylation of CREB, leading to decreased expression of specific channels and improved cardiac function.
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Arteriovenous fistulas (AVFs) are the most common vascular access points for hemodialysis (HD), but they have a high incidence of postoperative dysfunction, mainly due to excessive neointimal hyperplasia (NIH). Our previous studies have revealed a highly conserved LncRNA-LncDACH1 as an important regulator of cardiomyocyte and fibroblast proliferation. Herein, we find that LncDACH1 regulates NIH in AVF in male mice with conditional knockout of smooth muscle cell-specific LncDACH1 and in male mice model of AVF with LncDACH1 overexpression by adeno-associated virus.

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  • Cardiomyocyte maturation is essential for heart development, and abnormal maturation can lead to serious heart diseases, with Cfp1 being an under-researched factor in this process.
  • The study found that cardiomyocyte-specific Cfp1 knockout mice died within four weeks due to inhibited maturation, whereas Cfp1 transgenic mice and hiPSC-CMs with Cfp1 overexpression exhibited a more mature phenotype.
  • Cfp1 affects gene expression crucial for cardiomyocyte maturation by regulating H3K4me3 histone modification, indicating that its dysfunction is closely linked to cardiac diseases.
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  • Current atherosclerosis treatments struggle to reverse advanced plaque buildup, with high amino acid levels from a Western diet causing mTORC1-autophagy defects in macrophages, worsening the condition.* -
  • Research identified that the upregulation of Gpr137b-ps in atherosclerotic plaques impacts autophagy, with experiments on mice showing that reducing Gpr137b-ps enhances macrophage autophagy and reduces plaque lesions.* -
  • Gpr137b-ps seems to inhibit the binding of HSC70 to G3BP, which in turn affects mTORC1 signaling, suggesting targeting this pathway could offer a new avenue for treating advanced atherosclerosis.*
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Nonalcoholic fatty liver disease (NAFLD) is the most common liver disorder with high morbidity and mortality. The current study aims to explore the role of Cullin-associated and neddylation-dissociated protein 1 (CAND1) in the development of NAFLD and the underlying mechanisms. CAND1 is reduced in the liver of NAFLD male patients and high fat diet (HFD)-fed male mice.

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Speckle-type pox virus and zinc finger (POZ) protein (SPOP), a substrate recognition adaptor of cullin-3 (CUL3)/RING-type E3 ligase complex, is investigated for its role in cardiac fibrosis in our study. Cardiac fibroblasts (CFs) activation was achieved with TGF-β1 (20 ng/mL) and MI mouse model was established by ligation of the left anterior descending coronary, and lentivirus was employed to mediate interference of SPOP expression. SPOP was increased both in fibrotic post-MI mouse hearts and TGF-β1-treated CFs.

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Background: Atrial fibrillation (AF) is a highly prevalent condition that can cause or exacerbate heart failure, is an important risk factor for stroke, and is associated with pronounced morbidity and death. Genes uniquely expressed in the atria are known to be essential for maintaining atrial structure and function. Atrial tissue remodeling contributes to arrhythmia recurrence and maintenance.

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The incidence rate of atrial fibrillation (AF) has stayed at a high level in recent years. Despite the intensive efforts to study the pathologic changes of AF, the molecular mechanism of disease development remains unclarified. Microproteins are ribosomally translated gene products from small open reading frames (sORFs) and are found to play crucial biological functions, while remain rare attention and indistinct in AF study.

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  • Cardiac fibrosis is a key issue in heart diseases, leading to stiffness and dysfunction, and the long noncoding RNA (LncRNA) MetBil is being studied for its role in this process.
  • The study found that increased levels of MetBil in fibrotic heart tissue post-myocardial infarction (MI) promote collagen deposition and the proliferation of cardiac fibroblasts, while reducing its expression has the opposite, protective effects.
  • MetBil regulates the protein METTL3, impacting the expression of fibrosis-related genes in a way that could open new avenues for treating heart conditions associated with fibrosis.
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Objective: ASPP1 (apoptosis stimulating of p53 protein 1) is critical in regulating cell apoptosis as a cofactor of p53 to promote its transcriptional activity in the nucleus. However, whether cytoplasmic ASPP1 affects p53 nuclear trafficking and its role in cardiac diseases remains unknown. This study aims to explore the mechanism by which ASPP1 modulates p53 nuclear trafficking and the subsequent contribution to cardiac ischemia/reperfusion (I/R) injury.

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Non-healing diabetic wounds (DW) are a serious clinical problem that remained poorly understood. We recently found that topical application of growth differentiation factor 11 (GDF11) accelerated skin wound healing in both Type 1 DM (T1DM) and genetically engineered Type 2 diabetic db/db (T2DM) mice. In the present study, we elucidated the cellular and molecular mechanisms underlying the action of GDF11 on healing of small skin wound.

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Recent clinical trials indicate that sodium-glucose cotransporter 2 (SGLT2) inhibitors improve cardiovascular outcomes in myocardial infarction (MI) patients, but the underlying mechanisms remain unknown. As arrhythmia often occurs during myocardial infarction, it is the main cause of death. The purpose of this study was to investigate the influence of empagliflozin (EMPA), an SGLT2 inhibitor, on cardiac electrophysiological remodeling and arrhythmia susceptibility of myocardial infarction mice.

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Cancer-related epitopes can engage the immune system against tumor cells, thus exploring epitopes derived from non-coding regions is emerging as a fascinating field in cancer immunotherapies. Here, we described a database, IEAtlas (http://bio-bigdata.hrbmu.

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Article Synopsis
  • - The study focuses on myocardial ischemia/reperfusion (MI/R) injury, a serious issue for patients with coronary artery disease, aiming to discover key long non-coding RNAs (lncRNAs) involved in this process.
  • - Researchers identified a heart-specific lncRNA called lncCIRPIL, which was found to inhibit cell death in heart cells during oxygen deprivation and reintroduction (anoxia/reoxygenation) and suggests that its overexpression can protect against MI/R injury in mice.
  • - LncCIRPIL functions by sequestering the protein p53 in the cytoplasm, leading to its degradation, and this regulatory role could provide insights into new therapeutic strategies for MI/R injury.
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Pathological cardiac hypertrophy is a process characterized by significant disturbance of protein turnover. Cullin-associated and Neddylation-dissociated 1 (CAND1) acts as a coordinator to modulate substrate protein degradation by promoting the formation of specific cullin-based ubiquitin ligase 3 complex in response to substrate accumulation, which thereby facilitate the maintaining of normal protein homeostasis. Accumulation of calcineurin is critical in the pathogenesis of cardiac hypertrophy and heart failure.

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Dystrophin deficiency due to genetic mutations causes cardiac abnormalities in Duchenne's muscular dystrophy. Dystrophin is also shown to be downregulated in conventional failing hearts. Whether restoration of dystrophin expression possesses any therapeutic potential for conventional heart failure (HF) remains to be examined.

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Cardiac fibrosis is a process characterized by extracellular matrix accumulation leading to myocardial dysfunction. Angiotensin II (Ang II) has been shown to play an important role in the pathogenesis of cardiac fibrosis. However, the underlying mechanisms are not well established.

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Methyltransferase-like 3 (Mettl3) is a component of methyltransferase complex that mediates mA modification of RNAs, and participates in multiple biological processes. However, the role of Mettl3 in cardiac electrophysiology remains unknown. This study aims to explore the ventricular arrhythmia susceptibility of Mettl3 mice and the underlying mechanisms.

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Background: Ku70 participates in several pathological processes through mediating repair of DNA double-strand breaks. Our previous study has identified a highly conserved long noncoding RNA cardiac ischemia reperfusion associated Ku70 interacting lncRNA (CIRKIL) that was upregulated in myocardial infarction. The study aims to investigate whether CIRKIL regulates myocardial ischemia/reperfusion (I/R) through binding to Ku70.

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Diabetic cardiomyopathy (DCM) is a common complication in diabetic patients. The molecular mechanisms of DCM remain to be fully elucidated. The intronic long noncoding RNA of DACH1 (lncDACH1) has been demonstrated to be closely associated with heart failure and cardiac regeneration.

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N6-methyladenosine (mA), as the most abundant modification of mammalian messenger RNAs, is essential for tissue development and pathogenesis. However, the biological significance of mA methylation in cardiac differentiation and development remains largely unknown. Here, we identify that the downregulation of mA demethylase ALKBH5 is responsible for the increase of mA methylation and cardiomyocyte fate determination of human embryonic stem cells (hESCs) from mesoderm cells (MESs).

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