The Transcriptome-Wide Mapping of 2-Methylthio--isopentenyladenosine at Single-Base Resolution.

Hundreds of modified bases have been identified and enzymatically modified to transfer RNAs (tRNAs) to regulate RNA function in various organisms. 2-Methylthio--isopentenyladenosine (msiA), a hypermodified base found at tRNA position 37, exists in both prokaryotes and eukaryotes. msiA is traditionally identified by separating and digesting each tRNA from total RNA using RNA mass spectrometry.

Transcriptome-wide profiling of -methyladenosine a selective chemical labeling method.

Studies of chemical modifications on RNA have ushered in the field of epitranscriptomics. -Methyladenosine (mA) is the most typical RNA modification and is indispensable for basic biological processes. This study presents a chemical pulldown method (mA-ORL-Seq) for transcriptome-wide profiling of mA.

Inert Pepper aptamer-mediated endogenous mRNA recognition and imaging in living cells.

The development of RNA aptamers/fluorophores system is highly desirable for understanding the dynamic molecular biology of RNAs in vivo. Peppers-based imaging systems have been reported and applied for mRNA imaging in living cells. However, the need to insert corresponding RNA aptamer sequences into target RNAs and relatively low fluorescence signal limit its application in endogenous mRNA imaging.

6-Iodopurine as a Versatile Building Block for RNA Purine Architecture Modifications.

Natural modified bases in RNA were found to be indispensable for basic biological processes. In addition, artificial RNA modifications have been a versatile toolbox for the study of RNA interference, structure, and dynamics. Here, we present a chemical method for the facile synthesis of RNA containing C-modified purine.

Photoactive G-Quadruplex Ligand Identifies Multiple G-Quadruplex-Related Proteins with Extensive Sequence Tolerance in the Cellular Environment.

G-Quadruplex (G4) is a noncanonical nucleic acid secondary structure with multiple biofunctions. Identifying G4-related proteins (G4RPs) is important for understanding the roles of G4 in biology. Current methods to identify G4RPs include discovery from specific biological processes or pull-down assays with specific G4 sequences.

5-Formyluracil as a cornerstone for aluminum detection in vitro and in vivo: a more natural and sustainable strategy.

5-Formyluracil (5fU) based probes were designed and synthesized to detect Al3+ ions in vitro and in biological systems. These probes were synthesised using furan-2-carbohydrazide (fr) and 5fU derivatives. We also selectively labelled 5-formyluracil in DNA with the help of Al3+.