Enterococcus faecalis sir2-like gene enhances aerobic metabolism of themselves and mitochondrial respiration of mammal cells to bring about improving metabolic syndrome through the PGC-1α pathway.

Recent studies showed that probiotics could improve metabolic syndrome, making the identification of factors affecting metabolic control more important than ever. The mammalian sirtuin protein family has received much attention for its regulatory role, especially in various mitochondrial ATP, glucose, and lipid metabolic pathways. However, compared with the mammalian sirtuin protein family, the function of prokaryotic sir2 protein is much less known.

Oral engineered Bifidobacterium longum expressing rhMnSOD to suppress experimental colitis.

In recent years, using genetic engineering and bioengineering techniques, Bifidobacterium as a carrier to express specific functions of the protein or polypeptide, has become a new treatment for disease. Ulcerative colitis (UC) is a type of inflammatory bowel diseases (IBD). Although the cause of this inflammatory disorder is still unknown, a large amount of evidence suggests that ulcerative colitis is associated with increased activity of reactive oxygen species (ROS), manganese superoxide dismutase (MnSOD) is a kind of superoxide dismutase (SOD) has been demonstrated to play a key role in the pathophysiology of colitis.

Oral Bifidobacterium longum expressing GLP-2 improves nutrient assimilation and nutritional homeostasis in mice.

Bifidobacterium has been developed for the oral delivery of peptides and has the added beneficial effect on our bodies through its probiotic properties. Here, we utilize Bifidobacterium as a delivery system to orally deliver Glucagon like peptide-2 (GLP-2). We constructed vector derived from pET-31b(+) to construct a Bifidobacterium longum expressing GLP-2.

Anti-inflammatory properties of expressing human manganese superoxide dismutase using the TNBS-induced rats model of colitis.

The chronic enteritis disease ulcerative colitis (UC) is a formidable opponent, and its etiology remains unclear. Current studies have shown that oxidative stress is closely related to the pathogenesis of inflammatory bowel disease. Antioxidant enzymes, such as manganese superoxide dismutase (MnSOD)，have been deemed to exert an anti-inflammatory function.

The NAD-dependent deacetylase, Bifidobacterium longum Sir2 in response to oxidative stress by deacetylating SigH (σ) and FOXO3a in Bifidobacterium longum and HEK293T cell respectively.

Silent information regulator 2 (Sir2) enzymes which catalyze NAD+-dependent protein/histone deacetylation. The mammalian sirtuin family SIRT1, SIRT2, SIRT3 and SIRT6 can regulate oxidative stress. The probiotics (Bifidobacterium longum(B.

Effect of intervention using a messaging app on compliance and duration of treatment in orthodontic patients.

Objectives: This study aims to determine the effectiveness of a messaging app (WeChat) in improving patients' compliance and reducing the duration of orthodontic treatment (DOT).

Materials And Methods: A randomized controlled trial was performed in a dental hospital and a clinic from August 2012 to May 2015. Orthodontic patients were included at the beginning of treatment.

Factors Affecting the Clinical Success Rate of Miniscrew Implants for Orthodontic Treatment.

Purpose: The purpose of this study was to evaluate the various factors that influence the success rate of miniscrew implants used as orthodontic anchorage.

Materials And Methods: Potential confounding variables examined were sex, age, vertical (FMA) and sagittal (ANB) skeletal facial pattern, site of placement (labial and buccal, palatal, and retromandibular triangle), arch of placement (maxilla and mandible), placement soft tissue type, oral hygiene, diameter and length of miniscrew implants, insertion method (predrilled or drill-free), angle of placement, onset and strength of force application, and clinical purpose. The correlations between success rate and overall variables were investigated by logistic regression analysis, and the effect of each variable on the success rate was utilized by variance analysis.

Orthodontic mini-implant stability under continuous or intermittent loading: a histomorphometric and biomechanical analysis.

Background: Both continuous and intermittent loadings are commonly applied in orthodontics. Clinical experiences and some studies believed that longer duration of force produce more effect (tooth movement, suture expansion, bone remodeling) than transient forces applied with the same magnitude. Alternatively, others indicated that interruption or recovery periods of various periods between loadings cause more bone remodeling and less root resorption.

Coculture with endothelial cells enhances osteogenic differentiation of periodontal ligament stem cells via cyclooxygenase-2/prostaglandin E2/vascular endothelial growth factor signaling under hypoxia.

Background: During periodontitis and orthodontic tooth movement, periodontal vasculature is severely impaired, leading to a hypoxic microenvironment of periodontal cells. However, the impact of hypoxia on periodontal cells is poorly defined. The present study investigates responses of cocultured endothelial cells (ECs) and periodontal ligament stem cells (PDLSCs) to hypoxia.

Effect of placement angle on the stability of loaded titanium microscrews in beagle jaws.

Objective: To evaluate the effect of insertion angle on stability of loaded titanium microscrews in beagle jaws.

Materials And Methods: Forty-eight microscrews were inserted at four different angles (30°, 50°, 70°, and 90°) into the intraradicular zones of the mandibular first molars and third premolars of 12 beagles and immediately loaded with a force of 2 N for 8 weeks. Microcomputed tomography (micro-CT) and biomechanical pull-out tests were used to assess osseointegration of the interface.

Histomorphometric and biomechanical analyses of the osseointegration of loaded orthodontic microscrews inserted at different cortical bone thickness sites.

Objectives: The aim of this study was to evaluate the osseointegration of loaded orthodontic microscrews at different cortical bone thickness (CBT) sites.

Study Design: Forty-eight microscrews were inserted bilaterally in the tibias of 12 beagles, and divided into thick and thin CBT group. After 1, 3, 5, and 7 weeks, the dogs were killed and bone-screw specimens prepared for polyfluorochrome sequential labeling, microscopic computerized tomography (μCT) analysis, and biomechanical pull-out testing.

Up-regulated osteogenic transcription factors during early response of human periodontal ligament stem cells to cyclic tensile strain.

Introduction: As one group of periodontal ligament (PDL) cells, human periodontal ligament stem cells (hPDLSCs) have been isolated and identified as mesenchymal adult stem cells (MSCs) since 2004. It has been well accepted that PDL sensitively mediates the transmission of stress stimuli to the alveolar bone for periodontal tissue remolding. Besides, the direction of MSCs differentiation has been verified regulated by mechanical signals.

Bidimensional techniques for stronger anterior torque control in extraction cases: a combined clinical and typodont study.

Objective: To investigate the capacity of bidimensional techniques for torque control of anterior teeth in extraction cases.

Materials And Methods: Two different bidimensional techniques were distinguished by nomenclature as bidimensional-slot (bDS) and bidimensional-wire (bDW), respectively. (1) In the clinical study, patients were randomly assigned to three groups (ie, bDS [n  =  27], bDW [n  =  24], and control [n  =  25] groups).

Involvement of COX-2/PGE2 signalling in hypoxia-induced angiogenic response in endothelial cells.

To evaluate the impact of hypoxia on the angiogenic capability of endothelial cells (ECs), and further investigate whether the cyclooxygenase-2 (COX-2)/prostaglandin E(2) (PGE(2)) signalling is involved in the angiogenic response of ECs to hypoxia. We explored the impact of various periods (1, 3, 6, 12, 24 hrs) of hypoxia (2% O(2)) on human umbilical vein endothelial cells (HUVECs) in vitro. We observed cell viability, migration, tube formation, analysed COX-2, vascular endothelial growth factor (VEGF), AQP1 mRNA transcription, protein expression and measured PGE(2), VEGF protein concentration in cell supernatants.

Effect of placement angle on the stability of loaded titanium microscrews: a microcomputed tomographic and biomechanical analysis.

Introduction: The aim of this study was to evaluate the impact of the placement angle on the stability of loaded microscrews.

Methods: Forty-eight microscrews were placed at 4 angles ( 30°, 50°, 70°, and 90°) into the tibiae of 12 beagles, loaded with a force of 2 N immediately, and maintained for 8 weeks. Microcomputed tomography and pullout tests were used for morphometric and biomechanical analyses, respectively.

Effects of cortical bone thickness at different healing times on microscrew stability.

Objective: To compare the effects of different cortical bone thicknesses on the stability of microscrews at different healing times.

Materials: Sixty-four microscrews were inserted into the femurs of eight beagles, with four microscrews for one femur of one beagle dog. The dogs were sacrificed at 0, 3, 6, and 9 weeks after microscrew placement, respectively.

The effect of varying healing times on orthodontic mini-implant stability: a microscopic computerized tomographic and biomechanical analysis.

Objective: The aim of this study was to evaluate the effect of different healing times on the stability of titanium mini-implants used for orthodontic anchorage.

Study Design: Eight male beagles were used and randomized into 4 groups according to different healing times (1, 3, 5, and 7 weeks); each group had 2 beagles. Sixty-four mini-implants were inserted bilaterally in the maxilla and mandible of the beagles.

Orthodontic mini-implant stability in different healing times before loading: a microscopic computerized tomographic and biomechanical analysis.

Objective: The aim of this study was to evaluate the influence of different healing times before loading on the stability of implanted titanium mini-implant.

Study Design: Forty mini-implants were implanted in bilateral maxilla in 10 beagles, loaded with a force of 0.98 N after a delay of 0 day (immediately activated) and 1, 3, 5, and 7 weeks.

Quantitative research using computed tomographic scanning of beagle jaws for determination of safe zones for micro-screw implantation.

Objectives: The aim of this study was to provide an anatomical map for micro-screw placement in a safe location between the posterior dental roots of the beagle jaw.

Study Design: Three dimensional (3D) reconstructed images of 12 adult male beagles and jaw ground sections of another 6 beagles were examined. We measured sagittal, vertical and horizontal parameters in the intraradicular and interradicular regions of posterior teeth.