Comprehensive transcriptomic, proteomic, and intestinal microbiota analyses of largemouth bass (Micropterus salmoides) intestines reveal new insights into immune responses to Aeromonas hydrophila infection.

This study investigates the immune responses of largemouth bass (Micropterus salmoides) to infection by Aeromonas hydrophila, a pathogen responsible for significant economic losses in freshwater aquaculture due to bacterial enteritis. We employed transcriptomic sequencing, label-free LC-MS/MS quantitative proteomics, and 16S RNA sequencing to evaluate the transcriptomic, proteomic, and intestinal microbiota changes in infected fish compared to healthycontrols. Each fish (approximately 60 g) in the infection group was injected with 0.

Structural Regulation and Oxygen Reduction Reaction Activity of [Co(bpy)BO(OH)] and [Cu(bpy)(OH)] Complex Templated Borates.

Two templated borates, [Co(bpy)BO(OH)]·[BO(OH)]·HBO·HO·HO () and [Cu(bpy)(OH)]·[BO(OH)]·HO (), have been synthesized successfully and characterized by single-crystal X-ray diffraction, powder X-ray diffraction, and Fourier transform infrared. The [Co(bpy)BO(OH)] complex in shows a very rare coordination mode between Co and BO(OH). The structures of and can be adjusted by changing the reagent.

Single-Cell Ribonucleic Acid Sequencing Clarifies Cold Tolerance Mechanisms in the Pacific White Shrimp ().

To characterize the cold tolerance mechanism of the Pacific white shrimp (), we performed single-cell RNA sequencing (scRNA-seq) of ∼5185 hepatopancreas cells from cold-tolerant (Lv-T) and common (Lv-C) at preferred and low temperatures (28°C and 10°C, respectively). The cells fell into 10 clusters and 4 cell types: embryonic, resorptive, blister-like, and fibrillar. We identified differentially expressed genes between Lv-T and Lv-C, which were mainly associated with the terms "immune system," "cytoskeleton," "antioxidant system," "digestive enzyme," and "detoxification," as well as the pathways "metabolic pathways of oxidative phosphorylation," "metabolism of xenobiotics by cytochrome P450," "chemical carcinogenesis," "drug metabolism-cytochrome P450," and "fatty acid metabolism.

The Penaeus stylirostris densovirus capsid protein interacts with the Litopenaeus vannamei BCCIP protein.

Viral capsid proteins play an important role in the viral infection process. To identify the cellular proteins in shrimp that interact with the Penaeus stylirostris densovirus capsid protein (PstDNV-CP), we constructed a yeast two-hybrid (Y2H) cDNA library of the muscle tissue of Litopenaeus vannamei, and hybridized the bait vector pGBKT7-CP with this library. Cloning and sequencing showed that the shrimp protein interacting with PstDNV-CP was a homolog of BRCA2 and CDKN1A(p21)-interacting protein (BCCIP).

Transcriptome analysis of Litopenaeus vannamei in response to white spot syndrome virus infection.

Pacific white shrimp (Litopenaeus vannamei) is the most extensively farmed crustacean species in the world. White spot syndrome virus (WSSV) is one of the major pathogens in the cultured shrimp. However, the molecular mechanisms of the host-virus interaction remain largely unknown.

Transcriptome analysis of Pacific white shrimp (Litopenaeus vannamei) hepatopancreas in response to Taura syndrome Virus (TSV) experimental infection.

Background: The Pacific white shrimp, Litopenaeus vannamei, is a worldwide cultured crustacean species with important commercial value. Over the last two decades, Taura syndrome virus (TSV) has seriously threatened the shrimp aquaculture industry in the Western Hemisphere. To better understand the interaction between shrimp immune and TSV, we performed a transcriptome analysis in the hepatopancreas of L.