The effect of oxygen vacancies on the binding interactions of NH3 with rutile TiO2(110)-1 × 1.

A series of NH(3) temperature-programmed desorption (TPD) spectra were taken after dosing NH(3) at 70 K on rutile TiO(2)(110)-1 × 1 surfaces with oxygen vacancy (V(O)) concentrations of ~0% (p-TiO(2)) and 5% (r-TiO(2)), respectively, to study the effect of V(O)s on the desorption energy of NH(3) as a function of coverage, θ. Our results show that in the zero coverage limit, the desorption energy of NH(3) on r-TiO(2) is 115 kJ mol(-1), which is 10 kJ mol(-1) less than that on p-TiO(2). The desorption energy from the Ti(4+) sites decreases with increasing θ due to repulsive NH(3)-NH(3) interactions and approaches ~55 kJ mol(-1) upon the saturation of Ti(4+) sites (θ = 1 monolayer, ML) on both p- and r-TiO(2).

Identification of a divergent O-acetyltransferase gene oac 1b from Shigella flexneri serotype 1b strains.

Shigella flexneri is a leading cause of bacterial dysentery in developing countries. Among the 15 known serotypes, four (1b, 3a, 3b and 4b) contain a group 6 epitope due to an acetyl group connected to the O-2 position of rhamnose III on the tetrasaccharide structure of the lipopolysaccharide. O-acetyltransferase encoded by a bacteriophage, Sf6, mediates the acetylation reaction.

Alcohol Dehydration on Monooxo W═O and Dioxo O═W═O Species.

The dehydration of 1-propanol on nanoporous WO3 films prepared via ballistic deposition at ∼20 K has been investigated using temperature-programmed desorption, infrared reflection absorption spectroscopy, and density functional theory. The as-deposited films are extremely efficient in 1-propanol dehydration to propene. This activity is correlated with the presence of dioxo O═W═O groups, whereas monooxo W═O species are shown to be inactive.

Narrowband ultraviolet photodetector based on MgZnO and NPB heterojunction.

An ultraviolet photodetector was fabricated based on Mg0.07Zn0.93O heterojunction.

Emergence of a novel Shigella flexneri serotype 1d in China.

We report on the isolation of 5 Shigella flexneri strains displaying a novel serotype, 1d, that shares serologic features from both S. flexneri serotypes 1a and X. The 1d strains contained serotype-converting bacteriophages SfI and SfX in tandem on the chromosome.

[Development of a singleplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella flexneri].

Objective: To develop a singleplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella (S.) flexneri.

Methods: Eight pairs of primer for O-antigen synthesis and modification genes of S.

An outbreak of Mycoplasma pneumoniae caused by a macrolide-resistant isolate in a nursery school in China.

Eighteen out of 45 children were reported to have a respiratory illness during an outbreak at a temporary dormitory in a nursery school in China in 2011. To study the outbreak and to determine the risk factors for infection, an epidemiological investigation was performed. A standardized questionnaire was completed for a total of 45 children with the help of their guardians and parents.

Hemorrhagic fever caused by a novel Bunyavirus in China: pathogenesis and correlates of fatal outcome.

Background:  Hemorrhagic fever-like illness caused by a novel Bunyavirus, Huaiyangshan virus (HYSV, also known as Severe Fever with Thrombocytopenia virus [SFTSV] and Fever, Thrombocytopenia and Leukopenia Syndrome [FTLS]), has recently been described in China.

Methods:  Patients with laboratory-confirmed HYSV infection who were admitted to Union Hospital or Zhongnan Hospital between April 2010 and October 2010 were included in this study. Clinical and routine laboratory data were collected and blood, throat swab, urine, or feces were obtained when possible.

[Comparative proteomics of pancreatic cancer].

Objective: To identify differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues by comparative proteomics analysis.

Methods: Six pairs pancreatic tumor tissues and the adjacent noncancerous tissues were obtained and selected for two-dimensional polyacrylamide gel electrophoresis (2-DE) analysis. The differentially expressed proteins were identified using the PDQuest 2-D analysis software.

Development of a multiplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella flexneri.

Shigella flexneri is the major Shigella species that causes diarrheal disease in developing countries. It is further subdivided into 15 serotypes based on O-antigen structure. Serotyping of S.

Hemorrhagic fever caused by a novel tick-borne Bunyavirus in Huaiyangshan, China.

Background: From April to July in 2009 and 2010, unexplained severe hemorrhagic fever-like illnesses occurred in farmers from the Huaiyangshan mountains range.

Methods: Clinical specimens (blood, urine, feces, and throat swabs) from suspected patients were obtained and stored. Mosquitoes and ticks in affected regions were collected.

Solid pseudopapillary tumor of the pancreas: the surgical procedures.

Purpose: To evaluate the clinicopathological characteristics of solid pseudopapillary tumor and assess the curative effects of different procedures.

Methods: A retrospective review was performed in 34 patients with this tumor surgically treated between January 2000 and June 2008. These patients were divided into minimized and standard resection groups.

[Study on the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains].

Objective: To study the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains.

Methods: A series of primers were designed based on potential integration site of SfII and SfX prophages in Shigella flexneri serotype 2b strains, and PCR were performed for 50 serotype 2b strains to amplify special genes located in host and prophages. PCR products were sequenced to identify integration sites and arrangement of SfII and SfX.

Oxidation of Z α1-antitrypsin by cigarette smoke induces polymerization: a novel mechanism of early-onset emphysema.

The acceleration of chronic obstructive pulmonary disease (COPD) by cigarette smoke (CS) in individuals with severe genetic deficiency of α(1)-antitrypsin (Z-AT [Glu342Lys]) exemplifies the critical importance of gene-environmental interactions to the development of COPD. We investigated the molecular basis for the interaction between Z-AT and CS. Female mice (8-10 wk old) transgenic for normal (M-AT) or Z-AT on CBA background were exposed to four 1R3F cigarettes daily for 5 days.

The surface chemistry of dimethyl disulfide on copper.

The surface chemistry of dimethyl disulfide (DMDS) is studied on a Cu(111) single crystal and a polished copper foil in ultrahigh vacuum as a basis for understanding its tribological chemistry using a combination of temperature-programmed desorption (TPD), reflection-absorption infrared spectroscopy (RAIRS), and X-ray photoelectron spectroscopy (XPS). Low-energy electron diffraction reveals that the polished foil becomes ordered on heating in vacuo and displays identical surface chemistry to that found on the Cu(111) surface. Dimethyl disulfide reacts with the copper surface at 80 K to form thiolate species.

[Secretion expression of cholera toxin B subunit in food-grading Lactococcus lactis expression system].

Objective: To clone and secretion express cholera toxin B subunit (CTB) in food-grading Lactococcus lactis expression systems.

Methods: ctB fragment that encoding CTB was amplified by polymerase chain reaction (PCR) using the genomic DNA of Vibrio cholera strain 569B as template and was inserted into two secretion expression vector pSQZ and pSQ to construct food-grading expression system L.lactis MBP71/pSQZ-ctB and L.

[The POSSUM scoring system for risks assessment in pancreatoduodenectomy].

Objective: To explore the value of the POSSUM scoring system in predicting postoperative morbidity and mortality of pancreatoduodenectomy (PD).

Methods: Two hundreds and sixty-five consecutive PDs were performed between January 2005 and December 2007. POSSUM scores which relied on 12 physiologic and 6 operative variables were prospectively calculated for each case.

Emergence of a new multidrug-resistant serotype X variant in an epidemic clone of Shigella flexneri.

Shigella spp. are the causative agent of shigellosis with Shigella flexneri serotype 2a being the most prevalent in developing countries. Epidemiological surveillance in China found that a new serotype of S.

Oxidized {alpha}1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.

alpha(1)-Antitrypsin (AT) is a major elastase inhibitor within the lung. Oxidation of critical methionine residues in AT generates oxidized AT (Ox-AT), which has a greatly diminished ability to inhibit neutrophil elastase. This process may contribute to the pathogenesis of chronic obstructive pulmonary disease (COPD) by creating a functional deficiency of AT permitting lung destruction.

Pancreaticoduodenectomy with preoperative obstructive jaundice: drainage or not.

Objectives: In this study, we determined whether preoperative biliary drainage should be routinely performed in patients with jaundice.

Methods: The 342 patients undergoing pancreaticoduodenectomy between June 2004 and June 2008 were analyzed. Of these patients, 303 without biliary drainage were divided into 4 groups: (1) no jaundice, (2) mild jaundice, (3) moderate jaundice, and (4) severe jaundice.

[Clone and express ctb-stx2b fusion gene in Enterohemrrhagic escherichia coli O157:H7 Shigeal toxin 2B subunit and V cholera toxin B subunit and the detection of their immunogenicity].

Objective: To clone and express the fusion gene encoding Enterohemrrhagic escherichia coli O157 : H7 (EHEC O157 : H7) Shigela toxin 2B subunit (Stx2B) and vibrio cholera toxin B subunit (CTB) as well as to detect the immunogenicity and GM1-binding ability of fusion protein.

Methods: To design a primer to amplify stx2b gene and ctb-stx2b fusion gene encoding Stx2B and CTB-Stx2B respectively and to clone the genes into express plasmid pET30a(+)C in order to construct pET30a-ctb-stx2b after T-A sequencing was varified, then to transform constructed plasmid into E. coli BL21 (DE3) induced by IPTG and purified by a purify kit and to detect molecular weight and immunogenicity by SDS-PAGE and Western-blot.

[Molecular typing of isolates from two dysentery outbreaks in Chengdu, through pulsed field gel electrophoresis technology].

Objective: Using pulsed field gel electrophoresis (PFGE) and polymerase chain reaction (PCR) typing to analyze strains isolated from two outbreaks caused by Shigella sonnei and to trace the source of infection.

Methods: Virulence genes ipaH and ial were detected by PCR and PFGE was used to subtype the isolates. Patterns were compared, using the software BioNumerics.

Mechanism of cancer cell adaptation to metabolic stress: proteomics identification of a novel thyroid hormone-mediated gastric carcinogenic signaling pathway.

Gastric cancer is the second most common cancer worldwide and has a poor prognosis. To determine the mechanism of adaptation to metabolic stress in cancer cells, we used gastric cancer as a model system to reveal the potential signaling pathways involved. Two-dimensional polyacrylamide gel electrophoresis coupled with ESI-Q-TOF MS/MS analysis was used to identify differentially expressed proteins between gastric tumor tissues and the corresponding noncancerous tissues.

[Synthesis, characterization and antimicrobial activity of konjac glucomannan derivative with quaternary ammonium salts].

Methacryloxylethyl tetradecyl dimethyl ammonium bromide was grafted onto konjac glucomannan using ceric ammonium nitrate as an initiator, and the konjac glucomannan derivative with quaternary ammonium salts was obtained. The konjac glucomannan derivative was investigated by hydrogen nuclear magnetic resonance spectroscopy (1H NMR), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC), and Zeta sizer nano series. The antimicrobial properties of the konjac glucomannan derivative against selected microorganisms were tested by the quantitative suspension method.

[Analysis on the status of Shigella spp antimicrobial resistance through data from the National Shigellosis Surveillance System in China, in 2005].

Objective: The Ministry of Public Health released the National Surveillance project on Shigellosis in August, 2005. This study was to reveal the antimicrobial resistance status of Shigella isolates through the National Shigellosis Surveillance System in 2005 in China, so as to provide evidence for the development of surveillance, prevention and cure of Shigellosis.

Methods: All the lab assistants received training from Chinese Center for Disease Control and Prevention.